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While lipid mobilization has been implicated in the growth of <jats:italic>Plasmodium falciparum<\/jats:italic> malaria parasites in their <jats:italic>Anopheles<\/jats:italic> vectors, the role of this process in the reproductive biology of these mosquitoes remains elusive. Here, we show that impairing lipolysis in <jats:italic>Anopheles gambiae<\/jats:italic>, the major malaria vector, leads to embryonic lethality. Embryos derived from females in which we silenced the triglyceride lipase Ag<jats:italic>TL2<\/jats:italic> or the lipid storage droplet Ag<jats:italic>LSD1<\/jats:italic> develop normally during early embryogenesis but fail to hatch due to severely impaired metabolism. Embryonic lethality is efficiently recapitulated by exposing adult females to broad-spectrum lipase inhibitors prior to blood feeding, unveiling lipolysis as a potential target for inducing mosquito sterility. Our findings provide mechanistic insights into the importance of maternal lipid mobilization in embryonic health that may inform studies on human reproduction.<\/jats:p>","DOI":"10.1371\/journal.pbio.3002960","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T18:41:36Z","timestamp":1734460896000},"page":"e3002960","update-policy":"https:\/\/doi.org\/10.1371\/journal.pbio.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Maternal lipid mobilization is essential for embryonic development in the malaria vector Anopheles gambiae"],"prefix":"10.1371","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1356-0168","authenticated-orcid":true,"given":"Maurice A.","family":"Itoe","sequence":"first","affiliation":[]},{"given":"W. 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