{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T17:56:35Z","timestamp":1785779795462,"version":"3.56.0"},"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["431984000"],"award-info":[{"award-number":["431984000"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["239283807"],"award-info":[{"award-number":["239283807"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Germany's Excellence strategy","award":["390939984"],"award-info":[{"award-number":["390939984"]}]},{"name":"Germany's Excellence strategy","award":["390939984"],"award-info":[{"award-number":["390939984"]}]},{"DOI":"10.13039\/501100021729","name":"Medizinische Fakult\u00e4t der Albert-Ludwigs-Universit\u00e4t Freiburg","doi-asserted-by":"publisher","award":["Numbers 2023\/A2-Fol; 2021\/B3-Fol"],"award-info":[{"award-number":["Numbers 2023\/A2-Fol; 2021\/B3-Fol"]}],"id":[{"id":"10.13039\/501100021729","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["450392965"],"award-info":[{"award-number":["450392965"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"abstract":"<jats:p>Cell shape is a fundamental determinant of tissue architecture and organ function. In epithelial tissues, cytoskeletal organization and apical junctions regulate cell geometry, shaping functional tissue units. Disruption of these mechanisms is associated with diseases such as autosomal dominant polycystic kidney disease (ADPKD), in which epithelial organization is altered leading to cyst formation. Quantitative analysis of epithelial morphology can provide mechanistic insight, but existing approaches are often manual, low-throughput, and difficult to standardize. Here, we present a fully automated, deep learning-supported image analysis workflow for quantifying epithelial morphology in immunofluorescence images of zonula occludens protein 1 (ZO-1)-stained monolayers. Using a U-Net-based segmentation approach designed to mitigate out-of-focus regions, we extract standard cell shape features together with readouts tailored to the phenotype under study, including the R-index for junctional meandering and a border-based proxy for intercellular force transmission at shared cell-cell interfaces. We apply this workflow to genetically modified Madin-Darby canine kidney (MDCK) cell models of ADPKD and show that it captures genotype-associated differences in junctional organization that are not fully described by conventional shape descriptors alone. The workflow enables standardized, high-throughput phenotyping across large image datasets, reduces observer dependence, and supports analysis of mixed-cell experiments with genotype-resolved shared-border behavior. Together, these results establish a scalable framework for assay-specific quantification of epithelial morphology and junctional organization in defined experimental systems.<\/jats:p>","DOI":"10.1371\/journal.pcbi.1014614","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T17:37:49Z","timestamp":1785778669000},"page":"e1014614","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Deep learning-supported image quantification of epithelial cell shapes and its application to polycystic kidney disease"],"prefix":"10.1371","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0022-1957","authenticated-orcid":true,"given":"Johannes","family":"Jahn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexis","family":"Hofherr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Consoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berenike","family":"Fajen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebekka","family":"Goll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greta Theresa","family":"Liedtke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Boehm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friederike","family":"Selbach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul Christoph","family":"Zeisler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5220-7474","authenticated-orcid":true,"given":"Vanessa","family":"Weichselberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5157-0749","authenticated-orcid":true,"given":"Anne-Kathrin","family":"Classen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3525-3767","authenticated-orcid":true,"given":"Lukas","family":"Westermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3890-3427","authenticated-orcid":true,"given":"Tilman","family":"Busch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2406-5039","authenticated-orcid":true,"given":"Michael","family":"K\u00f6ttgen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"issue":"17","key":"pcbi.1014614.ref001","article-title":"Orchestrating morphogenesis: building the body plan by cell shape changes and movements","volume":"147","author":"KZ Perez-Vale","year":"2020","journal-title":"Development"},{"issue":"10","key":"pcbi.1014614.ref002","doi-asserted-by":"crossref","first-page":"1987","DOI":"10.1242\/dev.102228","article-title":"Apical constriction: themes and variations on a cellular mechanism driving morphogenesis","volume":"141","author":"AC Martin","year":"2014","journal-title":"Development"},{"issue":"3","key":"pcbi.1014614.ref003","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/S0092-8674(00)81279-9","article-title":"Cell adhesion: the molecular basis of tissue architecture and morphogenesis","volume":"84","author":"BM Gumbiner","year":"1996","journal-title":"Cell"},{"issue":"4","key":"pcbi.1014614.ref004","doi-asserted-by":"crossref","DOI":"10.1016\/j.devcel.2017.09.018","article-title":"Myosin II Controls Junction Fluctuations to Guide Epithelial Tissue Ordering","volume":"43","author":"S Curran","year":"2017","journal-title":"Dev Cell"},{"issue":"7228","key":"pcbi.1014614.ref005","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/nature07522","article-title":"Pulsed contractions of an actin-myosin network drive apical constriction","volume":"457","author":"AC Martin","year":"2009","journal-title":"Nature"},{"issue":"15","key":"pcbi.1014614.ref006","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.3390\/cells14151138","article-title":"Nonmuscle Myosin-2B Regulates Apical Cortical Mechanics, ZO-1 Dynamics and Cell Size in MDCK Epithelial Cells","volume":"14","author":"M Maup\u00e9rin","year":"2025","journal-title":"Cells"},{"issue":"1","key":"pcbi.1014614.ref007","doi-asserted-by":"crossref","first-page":"2514","DOI":"10.1038\/s41467-025-57428-y","article-title":"A feedback circuitry involving \u03b3-actin, \u03b2-actin and nonmuscle myosin-2\u2009A controls tight junction and apical cortex mechanics","volume":"16","author":"M Maup\u00e9rin","year":"2025","journal-title":"Nat Commun"},{"key":"pcbi.1014614.ref008","doi-asserted-by":"crossref","first-page":"1410","DOI":"10.1242\/jcs.045021","article-title":"Homophilic and heterophilic polycystin 1 interactions regulate E-cadherin recruitment and junction assembly in MDCK cells","volume":"122","author":"AJ Streets","year":"2009","journal-title":"J Cell Sci"},{"issue":"8","key":"pcbi.1014614.ref009","article-title":"Mechanosensing and Mechanotransduction at Cell-Cell Junctions","volume":"10","author":"AS Yap","year":"2018","journal-title":"Cold Spring Harb Perspect Biol"},{"issue":"7","key":"pcbi.1014614.ref010","doi-asserted-by":"crossref","DOI":"10.1083\/jcb.202208065","article-title":"Cingulin and paracingulin tether myosins-2 to junctions to mechanoregulate the plasma membrane","volume":"222","author":"F Rouaud","year":"2023","journal-title":"J Cell Biol"},{"issue":"8","key":"pcbi.1014614.ref011","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0104994","article-title":"ZO-1 knockout by TALEN-mediated gene targeting in MDCK cells: involvement of ZO-1 in the regulation of cytoskeleton and cell shape","volume":"9","author":"S Tokuda","year":"2014","journal-title":"PLoS One"},{"issue":"10","key":"pcbi.1014614.ref012","doi-asserted-by":"crossref","first-page":"2769","DOI":"10.1093\/hmg\/ddt672","article-title":"Polycystin-1 regulates actin cytoskeleton organization and directional cell migration through a novel PC1-Pacsin 2-N-Wasp complex","volume":"23","author":"G Yao","year":"2014","journal-title":"Hum Mol Genet"},{"issue":"23","key":"pcbi.1014614.ref013","doi-asserted-by":"crossref","first-page":"6253","DOI":"10.1111\/febs.70156","article-title":"Epithelial cell shape changes contribute to regulation of ureteric bud branching morphogenesis","volume":"292","author":"K Kurtzeborn","year":"2025","journal-title":"FEBS J"},{"issue":"2","key":"pcbi.1014614.ref014","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.devcel.2022.12.008","article-title":"The developing murine kidney actively negotiates geometric packing conflicts to avoid defects","volume":"58","author":"LS Prahl","year":"2023","journal-title":"Dev Cell"},{"issue":"9981","key":"pcbi.1014614.ref015","doi-asserted-by":"crossref","first-page":"1993","DOI":"10.1016\/S0140-6736(15)60907-2","article-title":"Autosomal dominant polycystic kidney disease: the changing face of clinical management","volume":"385","author":"ACM Ong","year":"2015","journal-title":"Lancet"},{"issue":"10174","key":"pcbi.1014614.ref016","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/S0140-6736(18)32782-X","article-title":"Autosomal dominant polycystic kidney disease","volume":"393","author":"E Cornec-Le Gall","year":"2019","journal-title":"Lancet"},{"issue":"1","key":"pcbi.1014614.ref017","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/s41572-018-0047-y","article-title":"Polycystic kidney disease","volume":"4","author":"C Bergmann","year":"2018","journal-title":"Nat Rev Dis Primers"},{"issue":"2","key":"pcbi.1014614.ref018","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1038\/ng0697-179","article-title":"PKD1 interacts with PKD2 through a probable coiled-coil domain","volume":"16","author":"F Qian","year":"1997","journal-title":"Nat Genet"},{"issue":"2","key":"pcbi.1014614.ref019","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1021\/bi0267792","article-title":"Polycystin-2 interacts with troponin I, an angiogenesis inhibitor","volume":"42","author":"Q Li","year":"2003","journal-title":"Biochemistry"},{"issue":"13","key":"pcbi.1014614.ref020","doi-asserted-by":"crossref","first-page":"6965","DOI":"10.1073\/pnas.94.13.6965","article-title":"Homo- and heterodimeric interactions between the gene products of PKD1 and PKD2","volume":"94","author":"L Tsiokas","year":"1997","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"6815","key":"pcbi.1014614.ref021","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1038\/35050128","article-title":"Co-assembly of polycystin-1 and -2 produces unique cation-permeable currents","volume":"408","author":"K Hanaoka","year":"2000","journal-title":"Nature"},{"issue":"2","key":"pcbi.1014614.ref022","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1038\/ng1076","article-title":"Polycystins 1 and 2 mediate mechanosensation in the primary cilium of kidney cells","volume":"33","author":"SM Nauli","year":"2003","journal-title":"Nat Genet"},{"issue":"18","key":"pcbi.1014614.ref023","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.262353","article-title":"Cingulin-nonmuscle myosin interaction plays a role in epithelial morphogenesis and cingulin nanoscale organization","volume":"137","author":"F Rouaud","year":"2024","journal-title":"J Cell Sci"},{"issue":"9","key":"pcbi.1014614.ref024","doi-asserted-by":"crossref","first-page":"183339","DOI":"10.1016\/j.bbamem.2020.183339","article-title":"Ruffles and spikes: Control of tight junction morphology and permeability by claudins","volume":"1862","author":"KS Lynn","year":"2020","journal-title":"Biochim Biophys Acta Biomembr"},{"key":"pcbi.1014614.ref025","first-page":"234","article-title":"U-Net: Convolutional Networks for Biomedical Image Segmentation.","volume-title":"Medical Image Computing and Computer-Assisted Intervention \u2013 MICCAI 2015","author":"O Ronneberger","year":"2015"},{"issue":"1","key":"pcbi.1014614.ref026","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/s41592-018-0261-2","article-title":"U-Net: deep learning for cell counting, detection, and morphometry","volume":"16","author":"T Falk","year":"2019","journal-title":"Nat Methods"},{"issue":"7553","key":"pcbi.1014614.ref027","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1038\/nature14539","article-title":"Deep learning","volume":"521","author":"Y LeCun","year":"2015","journal-title":"Nature"},{"key":"pcbi.1014614.ref028","article-title":"Cellpose-SAM: superhuman generalization for cellular segmentation","author":"M Pachitariu","year":"2025","journal-title":"bioRxiv"},{"key":"pcbi.1014614.ref029","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/978-1-4939-6371-3_13","article-title":"Segmentation and quantitative analysis of epithelial tissues","volume":"1478","author":"B Aigouy","year":"2016","journal-title":"Methods Mol Biol"},{"key":"pcbi.1014614.ref030","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.45413","article-title":"Junction Mapper is a novel computer vision tool to decipher cell-cell contact phenotypes","volume":"8","author":"H Brezovjakova","year":"2019","journal-title":"Elife"},{"issue":"1","key":"pcbi.1014614.ref031","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1038\/s41467-025-56643-x","article-title":"Polarity-JaM: an image analysis toolbox for cell polarity, junction and morphology quantification","volume":"16","author":"W Giese","year":"2025","journal-title":"Nat Commun"},{"issue":"6","key":"pcbi.1014614.ref032","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0217346","article-title":"TISMorph: A tool to quantify texture, irregularity and spreading of single cells","volume":"14","author":"E Alizadeh","year":"2019","journal-title":"PLoS One"},{"issue":"1","key":"pcbi.1014614.ref033","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1186\/s12859-021-04344-9","article-title":"CellProfiler 4: improvements in speed, utility and usability","volume":"22","author":"DR Stirling","year":"2021","journal-title":"BMC Bioinformatics"},{"key":"pcbi.1014614.ref034","author":"Wolfram Research","year":"2017"},{"issue":"17","key":"pcbi.1014614.ref035","article-title":"Response of Drosophila epithelial cell and tissue shape to external forces in vivo","volume":"146","author":"R Balaji","year":"2019","journal-title":"Development"},{"key":"pcbi.1014614.ref036","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.gde.2020.01.003","article-title":"Control of cell shape during epithelial morphogenesis: recent advances","volume":"63","author":"G Gillard","year":"2020","journal-title":"Curr Opin Genet Dev"},{"issue":"12","key":"pcbi.1014614.ref037","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1038\/s41588-021-00946-4","article-title":"Renal plasticity revealed through reversal of polycystic kidney disease in mice","volume":"53","author":"K Dong","year":"2021","journal-title":"Nat Genet"},{"issue":"4","key":"pcbi.1014614.ref038","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1172\/JCI22850","article-title":"Pkd1 regulates immortalized proliferation of renal tubular epithelial cells through p53 induction and JNK activation","volume":"115","author":"S Nishio","year":"2005","journal-title":"J Clin Invest"},{"issue":"18","key":"pcbi.1014614.ref039","doi-asserted-by":"crossref","DOI":"10.1242\/dev.199616","article-title":"Deep learning for bioimage analysis in developmental biology","volume":"148","author":"A Hallou","year":"2021","journal-title":"Development"},{"issue":"8","key":"pcbi.1014614.ref040","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1177\/2472555217716056","article-title":"High-Throughput Phenotypic Screening of Kinase Inhibitors to Identify Drug Targets for Polycystic Kidney Disease","volume":"22","author":"TH Booij","year":"2017","journal-title":"SLAS Discov"},{"issue":"1","key":"pcbi.1014614.ref041","doi-asserted-by":"crossref","first-page":"4203","DOI":"10.1038\/s41598-020-61082-3","article-title":"A high-throughput screening platform for Polycystic Kidney Disease (PKD) drug repurposing utilizing murine and human ADPKD cells","volume":"10","author":"RR Asawa","year":"2020","journal-title":"Sci Rep"},{"issue":"5","key":"pcbi.1014614.ref042","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1038\/nmeth.3834","article-title":"Measuring cell-generated forces: a guide to the available tools","volume":"13","author":"WJ Polacheck","year":"2016","journal-title":"Nat Methods"},{"issue":"9","key":"pcbi.1014614.ref043","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.3390\/met14091051","article-title":"Efficient Phase Segmentation of Light-Optical Microscopy Images of Highly Complex Microstructures Using a Correlative Approach in Combination with Deep Learning Techniques","volume":"14","author":"B-I Bachmann","year":"2024","journal-title":"Metals"},{"key":"pcbi.1014614.ref044","doi-asserted-by":"crossref","first-page":"75","DOI":"10.3389\/fninf.2019.00075","article-title":"QUINT: Workflow for Quantification and Spatial Analysis of Features in Histological Images From Rodent Brain","volume":"13","author":"SC Yates","year":"2019","journal-title":"Front Neuroinform"},{"issue":"6","key":"pcbi.1014614.ref045","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1093\/bib\/bbv004","article-title":"Detecting and overcoming systematic bias in high-throughput screening technologies: a comprehensive review of practical issues and methodological solutions","volume":"16","author":"I Caraus","year":"2015","journal-title":"Brief Bioinform"},{"issue":"1","key":"pcbi.1014614.ref046","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1083\/jcb.146.1.219","article-title":"Recycling of E-cadherin: a potential mechanism for regulating cadherin dynamics","volume":"146","author":"TL Le","year":"1999","journal-title":"J Cell Biol"},{"issue":"7","key":"pcbi.1014614.ref047","doi-asserted-by":"crossref","first-page":"2361","DOI":"10.1534\/g3.118.200233","article-title":"Identifying Genetic Players in Cell Sheet Morphogenesis Using a Drosophila Deficiency Screen for Genes on Chromosome 2R Involved in Dorsal Closure","volume":"8","author":"RD Mortensen","year":"2018","journal-title":"G3 (Bethesda)"},{"key":"pcbi.1014614.ref048","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.omtm.2021.05.005","article-title":"Monolayer platform using human biopsy-derived duodenal organoids for pharmaceutical research","volume":"22","author":"T Yamashita","year":"2021","journal-title":"Mol Ther Methods Clin Dev"},{"key":"pcbi.1014614.ref049","doi-asserted-by":"crossref","first-page":"109646","DOI":"10.1016\/j.cellsig.2020.109646","article-title":"Extracellular matrix, integrins, and focal adhesion signaling in polycystic kidney disease","volume":"72","author":"Y Zhang","year":"2020","journal-title":"Cell Signal"},{"key":"pcbi.1014614.ref050","doi-asserted-by":"crossref","first-page":"103138","DOI":"10.1016\/j.micron.2021.103138","article-title":"Traction force microscopy - Measuring the forces exerted by cells","volume":"150","author":"M Lekka","year":"2021","journal-title":"Micron"},{"issue":"21","key":"pcbi.1014614.ref051","doi-asserted-by":"crossref","first-page":"14854","DOI":"10.1074\/jbc.M114.562264","article-title":"N-glycosylation determines the abundance of the transient receptor potential channel TRPP2","volume":"289","author":"A Hofherr","year":"2014","journal-title":"J Biol Chem"},{"issue":"9","key":"pcbi.1014614.ref052","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1016\/j.mri.2012.05.001","article-title":"3D Slicer as an image computing platform for the Quantitative Imaging Network","volume":"30","author":"A Fedorov","year":"2012","journal-title":"Magn Reson Imaging"}],"container-title":["PLOS Computational Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pcbi.1014614","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T17:37:59Z","timestamp":1785778679000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pcbi.1014614"}},"subtitle":[],"editor":[{"given":"Virginie","family":"Uhlmann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,8,3]]},"references-count":52,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8,3]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pcbi.1014614","relation":{},"ISSN":["1553-7358"],"issn-type":[{"value":"1553-7358","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,3]]}}}