{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T23:25:38Z","timestamp":1773962738684,"version":"3.50.1"},"update-to":[{"DOI":"10.1371\/journal.pgen.1009856","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,11,5]],"date-time":"2021-11-05T00:00:00Z","timestamp":1636070400000}}],"reference-count":94,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T00:00:00Z","timestamp":1634774400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["824.14.021"],"award-info":[{"award-number":["824.14.021"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["016.VICI.170.165"],"award-info":[{"award-number":["016.VICI.170.165"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["675407\u2013 PolarNet"],"award-info":[{"award-number":["675407\u2013 PolarNet"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["675407\u2013 PolarNet"],"award-info":[{"award-number":["675407\u2013 PolarNet"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    The conserved adapter protein Scribble (Scrib) plays essential roles in a variety of cellular processes, including polarity establishment, proliferation, and directed cell migration. While the mechanisms through which Scrib promotes epithelial polarity are beginning to be unraveled, its roles in other cellular processes including cell migration remain enigmatic. In\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>elegans<\/jats:italic>\n                    , the Scrib ortholog LET-413 is essential for apical\u2013basal polarization and junction formation in embryonic epithelia. However, whether LET-413 is required for postembryonic development or plays a role in migratory events is not known. Here, we use inducible protein degradation to investigate the functioning of LET-413 in larval epithelia. We find that LET-413 is essential in the epidermal epithelium for growth, viability, and junction maintenance. In addition, we identify a novel role for LET-413 in the polarized outgrowth of the epidermal seam cells. These stem cell-like epithelial cells extend anterior and posterior directed apical protrusions in each larval stage to reconnect to their neighbors. We show that the role of LET-413 in seam cell outgrowth is likely mediated largely by the junctional component DLG-1 discs large, which we demonstrate is also essential for directed outgrowth of the seam cells. Our data uncover multiple essential functions for LET-413 in larval development and show that the polarized outgrowth of the epithelial seam cells is controlled by LET-413 Scribble and DLG-1 Discs large.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1009856","type":"journal-article","created":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T14:14:23Z","timestamp":1634825663000},"page":"e1009856","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Caenorhabditis elegans LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells"],"prefix":"10.1371","volume":"17","author":[{"given":"Amalia","family":"Riga","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6809-2587","authenticated-orcid":true,"given":"Janine","family":"Cravo","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9187-5424","authenticated-orcid":true,"given":"Ruben","family":"Schmidt","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8351-0812","authenticated-orcid":true,"given":"Helena R.","family":"Pires","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9884-2537","authenticated-orcid":true,"given":"Victoria G.","family":"Castiglioni","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9015-7463","authenticated-orcid":true,"given":"Sander","family":"van den Heuvel","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3966-4173","authenticated-orcid":true,"given":"Mike","family":"Boxem","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2021,10,21]]},"reference":[{"key":"pgen.1009856.ref001","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/S0092-8674(03)00938-3","article-title":"Drosophila PAR-1 and 14-3-3 Inhibit Bazooka\/PAR-3 to Establish Complementary Cortical Domains in Polarized Cells","volume":"115","author":"R Benton","year":"2003","journal-title":"Cell"},{"key":"pgen.1009856.ref002","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1038\/ncb897","article-title":"Integrated activity of PDZ protein complexes regulates epithelial polarity","volume":"5","author":"D Bilder","year":"2003","journal-title":"Nat Cell 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