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King fellowship"],"award-info":[{"award-number":["Charles A. King fellowship"]}],"id":[{"id":"10.13039\/100009158","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>Females from many mosquito species feed on blood to acquire nutrients for egg development. The oogenetic cycle has been characterized in the arboviral vector <jats:italic>Aedes aegypti<\/jats:italic>, where after a bloodmeal, the lipid transporter lipophorin (Lp) shuttles lipids from the midgut and fat body to the ovaries, and a yolk precursor protein, vitellogenin (Vg), is deposited into the oocyte by receptor-mediated endocytosis. Our understanding of how the roles of these two nutrient transporters are mutually coordinated is however limited in this and other mosquito species. Here, we demonstrate that in the malaria mosquito <jats:italic>Anopheles gambiae<\/jats:italic>, Lp and Vg are reciprocally regulated in a timely manner to optimize egg development and ensure fertility. Defective lipid transport via <jats:italic>Lp<\/jats:italic> knockdown triggers abortive ovarian follicle development, leading to misregulation of Vg and aberrant yolk granules. Conversely, depletion of Vg causes an upregulation of <jats:italic>Lp<\/jats:italic> in the fat body in a manner that appears to be at least partially dependent on target of rapamycin (TOR) signaling, resulting in excess lipid accumulation in the developing follicles. Embryos deposited by Vg-depleted mothers are completely inviable, and are arrested early during development, likely due to severely reduced amino acid levels and protein synthesis. Our findings demonstrate that the mutual regulation of these two nutrient transporters is essential to safeguard fertility by ensuring correct nutrient balance in the developing oocyte, and validate Vg and Lp as two potential candidates for mosquito control.<\/jats:p>","DOI":"10.1371\/journal.pgen.1011145","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T18:33:40Z","timestamp":1706553220000},"page":"e1011145","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Precise coordination between nutrient transporters ensures fertility in the malaria mosquito Anopheles gambiae"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2611-9558","authenticated-orcid":true,"given":"Iryna","family":"Stryapunina","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1356-0168","authenticated-orcid":true,"given":"Maurice A.","family":"Itoe","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6933-6529","authenticated-orcid":true,"given":"Queenie","family":"Trinh","sequence":"additional","affiliation":[]},{"given":"Charles","family":"Vidoudez","sequence":"additional","affiliation":[]},{"given":"Esrah","family":"Du","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6522-0501","authenticated-orcid":true,"given":"Lydia","family":"Mendoza","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3207-1260","authenticated-orcid":true,"given":"Oleksandr","family":"Hulai","sequence":"additional","affiliation":[]},{"given":"Jamie","family":"Kauffman","sequence":"additional","affiliation":[]},{"given":"John","family":"Carew","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2806-9718","authenticated-orcid":true,"given":"W. Robert","family":"Shaw","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3295-6683","authenticated-orcid":true,"given":"Flaminia","family":"Catteruccia","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2024,1,29]]},"reference":[{"key":"pgen.1011145.ref001","volume-title":"World Malaria Report","year":"2022"},{"issue":"7","key":"pgen.1011145.ref002","doi-asserted-by":"crossref","first-page":"3967","DOI":"10.1074\/jbc.273.7.3967","article-title":"Identification of a steroidogenic neurohormone in female mosquitoes","volume":"273","author":"MR Brown","year":"1998","journal-title":"J Biol Chem"},{"issue":"15","key":"pgen.1011145.ref003","doi-asserted-by":"crossref","first-page":"5716","DOI":"10.1073\/pnas.0800478105","article-title":"An insulin-like peptide regulates egg maturation and metabolism in the mosquito Aedes aegypti","volume":"105","author":"MR Brown","year":"2008","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"7","key":"pgen.1011145.ref004","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.ibmb.2005.02.013","article-title":"Nutritional regulation of vitellogenesis in mosquitoes: implications for anautogeny","volume":"35","author":"GM Attardo","year":"2005","journal-title":"Insect Biochem Mol Biol"},{"issue":"2","key":"pgen.1011145.ref005","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1083\/jcb.20.2.313","article-title":"Yolk Protein Uptake in the Oocyte of the Mosquito Aedes aegypti. 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Drosophila melanogaster with depressed numbers of coated pits and coated vesicles within the developing oocytes","volume":"105","author":"PJ DiMario","year":"1987","journal-title":"J Cell Biol"},{"issue":"2","key":"pgen.1011145.ref031","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/BF00319427","article-title":"Blocked endocytotic uptake by the oocyte causes accumulation of vitellogenins in the haemolymph of the female-sterile mutants quitPX61 and stand stillPS34 of Drosophila","volume":"275","author":"HO Gutzeit","year":"1994","journal-title":"Cell Tissue Res"},{"issue":"19","key":"pgen.1011145.ref032","doi-asserted-by":"crossref","first-page":"13345","DOI":"10.1074\/jbc.M113.462556","article-title":"Vitellogenin receptor mutation leads to the oogenesis mutant phenotype \"scanty vitellin\" of the silkworm, Bombyx mori.","volume":"288","author":"Y Lin","year":"2013","journal-title":"J Biol 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