{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T10:38:25Z","timestamp":1760783905964},"reference-count":58,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2015,1,8]],"date-time":"2015-01-08T00:00:00Z","timestamp":1420675200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0003430","type":"journal-article","created":{"date-parts":[[2015,1,8]],"date-time":"2015-01-08T19:14:43Z","timestamp":1420744483000},"page":"e3430","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":20,"title":["Ribose 5-Phosphate Isomerase B Knockdown Compromises Trypanosoma brucei Bloodstream Form Infectivity"],"prefix":"10.1371","volume":"9","author":[{"given":"In\u00eas","family":"Loureiro","sequence":"first","affiliation":[]},{"given":"Joana","family":"Faria","sequence":"additional","affiliation":[]},{"given":"Christine","family":"Clayton","sequence":"additional","affiliation":[]},{"given":"Sandra","family":"Macedo-Ribeiro","sequence":"additional","affiliation":[]},{"given":"Nuno","family":"Santar\u00e9m","sequence":"additional","affiliation":[]},{"given":"Nilanjan","family":"Roy","sequence":"additional","affiliation":[]},{"given":"Anabela","family":"Cordeiro-da-Siva","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Tavares","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2015,1,8]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1038\/nrmicro2779","article-title":"Trypanosomal immune evasion, chronicity and transmission: an elegant balancing act","volume":"10","author":"P MacGregor","year":"2012","journal-title":"Nat Rev Microbiol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"e1000078","DOI":"10.1371\/journal.ppat.1000078","article-title":"Trypanosomiasis-induced B cell apoptosis results in loss of protective anti-parasite antibody responses and abolishment of vaccine-induced memory responses","volume":"4","author":"M Radwanska","year":"2008","journal-title":"PLoS Pathog"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"e1003947","DOI":"10.1371\/journal.ppat.1003947","article-title":"Gambiense human african trypanosomiasis and immunological memory: effect on phenotypic lymphocyte profiles and humoral immunity","volume":"10","author":"V Lejon","year":"2014","journal-title":"PLoS Pathog"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/S0169-4758(96)10073-9","article-title":"Metabolic compartmentation in African trypanosomes","volume":"12","author":"CE Clayton","year":"1996","journal-title":"Parasitol Today"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/S0065-2911(05)50005-5","article-title":"Energy metabolism and its compartmentation in Trypanosoma brucei","volume":"50","author":"JJ Hellemond","year":"2005","journal-title":"Adv Microb Physiol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"28306","DOI":"10.1074\/jbc.M502403200","article-title":"Experimental and in silico analyses of glycolytic flux control in bloodstream form Trypanosoma brucei","volume":"280","author":"MA Albert","year":"2005","journal-title":"J Biol Chem"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.molbiopara.2007.12.013","article-title":"The anti-trypanosomal agent lonidamine inhibits Trypanosoma brucei hexokinase 1","volume":"158","author":"JW Chambers","year":"2008","journal-title":"Mol Biochem Parasitol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1016\/S1074-5521(02)00169-2","article-title":"Sequencing, modeling, and selective inhibition of Trypanosoma brucei hexokinase","volume":"9","author":"M Willson","year":"2002","journal-title":"Chem Biol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1006\/jmbi.2000.3910","article-title":"Structures of type 2 peroxisomal targeting signals in two trypanosomatid aldolases","volume":"300","author":"DM Chudzik","year":"2000","journal-title":"J Mol Biol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"3440","DOI":"10.1016\/j.bmcl.2006.04.010","article-title":"Cell permeation of a Trypanosoma brucei aldolase inhibitor: evaluation of different enzyme-labile phosphate protecting groups","volume":"16","author":"L Azema","year":"2006","journal-title":"Bioorg Med Chem Lett"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.molbiopara.2009.09.001","article-title":"Genetic validation of aldolase and glyceraldehyde-3-phosphate dehydrogenase as drug targets in Trypanosoma brucei","volume":"169","author":"AJ Caceres","year":"2010","journal-title":"Mol Biochem Parasitol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1042\/bj3570117","article-title":"Roles of triosephosphate isomerase and aerobic metabolism in Trypanosoma brucei","volume":"357","author":"S Helfert","year":"2001","journal-title":"Biochem J"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"4273","DOI":"10.1073\/pnas.96.8.4273","article-title":"Structure-based design of submicromolar, biologically active inhibitors of trypanosomatid glyceraldehyde-3-phosphate dehydrogenase","volume":"96","author":"AM Aronov","year":"1999","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1128\/EC.00141-06","article-title":"Chromosome-wide analysis of gene function by RNA interference in the african trypanosome","volume":"5","author":"C Subramaniam","year":"2006","journal-title":"Eukaryot Cell"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"4135","DOI":"10.1021\/jm000287a","article-title":"Adenosine analogues as inhibitors of Trypanosoma brucei phosphoglycerate kinase: elucidation of a novel binding mode for a 2-amino-N(6)-substituted adenosine","volume":"43","author":"JC Bressi","year":"2000","journal-title":"J Med Chem"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.1016\/j.bmc.2009.01.068","article-title":"Inhibition of Trypanosoma brucei glucose-6-phosphate dehydrogenase by human steroids and their effects on the viability of cultured parasites","volume":"17","author":"AT Cordeiro","year":"2009","journal-title":"Bioorg Med Chem"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"3427","DOI":"10.1021\/jm031066i","article-title":"Selective inhibition of Trypanosoma brucei 6-phosphogluconate dehydrogenase by high-energy intermediate and transition-state analogues","volume":"47","author":"C Dardonville","year":"2004","journal-title":"J Med Chem"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/0014-5793(89)81154-8","article-title":"The enzymes of the classical pentose phosphate pathway display differential activities in procyclic and bloodstream forms of Trypanosoma brucei","volume":"244","author":"CN Cronin","year":"1989","journal-title":"FEBS Lett"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Comini MA OC, Cazzulo JJ (2013) Drug Targets in Trypanosomal and Leishmanial Pentose Phosphate Pathway. In: J\u00e4ger T KO, Floh\u00e9 L, editor. Trypanosomatid Diseases: Molecular Routes to Drug Discovery. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. pp. 297\u2013313.","DOI":"10.1002\/9783527670383.ch16"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"e1831","DOI":"10.1371\/journal.pntd.0001831","article-title":"Dramatic increase in glycerol biosynthesis upon oxidative stress in the anaerobic protozoan parasite Entamoeba histolytica","volume":"6","author":"A Husain","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/jbiol61","article-title":"Dynamic rerouting of the carbohydrate flux is key to counteracting oxidative stress","volume":"6","author":"M Ralser","year":"2007","journal-title":"J Biol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1128\/jb.178.4.1003-1011.1996","article-title":"Ribose catabolism of Escherichia coli: characterization of the rpiB gene encoding ribose phosphate isomerase B and of the rpiR gene, which is involved in regulation of rpiB expression","volume":"178","author":"KI Sorensen","year":"1996","journal-title":"J Bacteriol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"14385","DOI":"10.1074\/jbc.M413338200","article-title":"Substrate specificity, localization, and essential role of the glutathione peroxidase-type tryparedoxin peroxidases in Trypanosoma brucei","volume":"280","author":"T Schlecker","year":"2005","journal-title":"J Biol Chem"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.molbiopara.2004.10.002","article-title":"A doubly inducible system for RNA interference and rapid RNAi plasmid construction in Trypanosoma brucei","volume":"139","author":"VP Alibu","year":"2005","journal-title":"Mol Biochem Parasitol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","article-title":"Clustal W and Clustal X version 2.0","volume":"23","author":"MA Larkin","year":"2007","journal-title":"Bioinformatics"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1107\/S0907444909007835","article-title":"ALINE: a WYSIWYG protein-sequence alignment editor for publication-quality alignments","volume":"65","author":"CS Bond","year":"2009","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1093\/bioinformatics\/bti770","article-title":"The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling","volume":"22","author":"K Arnold","year":"2006","journal-title":"Bioinformatics"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"D387","DOI":"10.1093\/nar\/gkn750","article-title":"The SWISS-MODEL Repository and associated resources","volume":"37","author":"F Kiefer","year":"2009","journal-title":"Nucleic Acids Res"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/S0968-0004(00)88963-X","article-title":"The Swiss-3DImage collection and PDB-Browser on the World-Wide Web","volume":"20","author":"MC Peitsch","year":"1995","journal-title":"Trends Biochem Sci"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/0003-2697(70)90300-3","article-title":"Spectrophotometric ass for D-ribose-5-phosphateketol-isomerase and for D-ribulose-5-phosphate 3-epimerase","volume":"33","author":"T Wood","year":"1970","journal-title":"Anal Biochem"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1042\/BJ20061049","article-title":"Ribose 5-phosphate isomerase type B from Trypanosoma cruzi: kinetic properties and site-directed mutagenesis reveal information about the reaction mechanism","volume":"401","author":"AL Stern","year":"2007","journal-title":"Biochem J"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1515\/bchm2.1974.355.2.781","article-title":"Protein structure and enzymatic activity, XIV. Purification and properties of ribosephosphate isomerase from skeletal muscle","volume":"355","author":"GF Domagk","year":"1974","journal-title":"Hoppe Seylers Z Physiol Chem"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.molbiopara.2007.02.008","article-title":"Highly efficient stable transformation of bloodstream forms of Trypanosoma brucei","volume":"153","author":"G Burkard","year":"2007","journal-title":"Mol Biochem Parasitol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"9781","DOI":"10.1074\/jbc.M600133200","article-title":"The RACK1 homologue from Trypanosoma brucei is required for the onset and progression of cytokinesis","volume":"281","author":"KG Rothberg","year":"2006","journal-title":"J Biol Chem"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1038\/2181273a0","article-title":"Separation of trypanosomes from the blood of infected rats and mice by anion-exchangers","volume":"218","author":"SM Lanham","year":"1968","journal-title":"Nature"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1111\/j.1742-4658.2010.07999.x","article-title":"Structures of type B ribose 5-phosphate isomerase from Trypanosoma cruzi shed light on the determinants of sugar specificity in the structural family","volume":"278","author":"AL Stern","year":"2011","journal-title":"FEBS J"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"6416","DOI":"10.1074\/jbc.M412018200","article-title":"Competitive inhibitors of Mycobacterium tuberculosis ribose-5-phosphate isomerase B reveal new information about the reaction mechanism","volume":"280","author":"AK Roos","year":"2005","journal-title":"J Biol Chem"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"2649","DOI":"10.1083\/jcb.105.6.2649","article-title":"Import of fructose bisphosphate aldolase into the glycosomes of Trypanosoma brucei","volume":"105","author":"CE Clayton","year":"1987","journal-title":"J Cell Biol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S0166-6851(02)00276-1","article-title":"Development of RNA interference revertants in Trypanosoma brucei cell lines generated with a double stranded RNA expression construct driven by two opposing promoters","volume":"126","author":"Y Chen","year":"2003","journal-title":"Mol Biochem Parasitol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.molbiopara.2004.12.009","article-title":"Trypanosoma brucei RNA interference in the mammalian host","volume":"140","author":"L Lecordier","year":"2005","journal-title":"Mol Biochem Parasitol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1111\/j.1365-2958.2009.06657.x","article-title":"The cell cycle as a therapeutic target against Trypanosoma brucei: Hesperadin inhibits Aurora kinase-1 and blocks mitotic progression in bloodstream forms","volume":"72","author":"N Jetton","year":"2009","journal-title":"Mol Microbiol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"e2578","DOI":"10.1371\/journal.pntd.0002578","article-title":"Knockdown of asparagine synthetase A renders Trypanosoma brucei auxotrophic to asparagine","volume":"7","author":"I Loureiro","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.bbrc.2012.03.107","article-title":"Identification and characterization of a novel Ribose 5-phosphate isomerase B from Leishmania donovani","volume":"421","author":"PK Kaur","year":"2012","journal-title":"Biochem Biophys Res Commun"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.jbiotec.2008.09.012","article-title":"Novel substrates of a ribose-5-phosphate isomerase from Clostridium thermocellum","volume":"139","author":"RY Yoon","year":"2009","journal-title":"J Biotechnol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1104\/pp.97.2.730","article-title":"Purification and characterization of pea chloroplastic phosphoriboisomerase","volume":"97","author":"CL Skrukrud","year":"1991","journal-title":"Plant Physiol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1186\/1471-2164-10-482","article-title":"Widespread variation in transcript abundance within and across developmental stages of Trypanosoma brucei","volume":"10","author":"BC Jensen","year":"2009","journal-title":"BMC Genomics"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"3275","DOI":"10.1002\/pmic.200500668","article-title":"Comparative proteomics of glycosomes from bloodstream form and procyclic culture form Trypanosoma brucei brucei","volume":"6","author":"C Colasante","year":"2006","journal-title":"Proteomics"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.molbiopara.2006.02.010","article-title":"In silico prediction of the glycosomal enzymes of Leishmania major and trypanosomes","volume":"147","author":"FR Opperdoes","year":"2006","journal-title":"Mol Biochem Parasitol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.molbiopara.2011.04.006","article-title":"Transketolase in Trypanosoma brucei","volume":"179","author":"SA Stoffel","year":"2011","journal-title":"Mol Biochem Parasitol"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"27559","DOI":"10.1074\/jbc.M004266200","article-title":"Molecular characterization of the first two enzymes of the pentose-phosphate pathway of Trypanosoma brucei. Glucose-6-phosphate dehydrogenase and 6-phosphogluconolactonase","volume":"275","author":"F Duffieux","year":"2000","journal-title":"J Biol Chem"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"e1003371","DOI":"10.1371\/journal.pcbi.1003371","article-title":"Handling uncertainty in dynamic models: the pentose phosphate pathway in Trypanosoma brucei","volume":"9","author":"EJ Kerkhoven","year":"2013","journal-title":"PLoS Comput Biol"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1111\/mmi.12376","article-title":"Trypanosoma brucei (UMP synthase null mutants) are avirulent in mice, but recover virulence upon prolonged culture in vitro while retaining pyrimidine auxotrophy","volume":"90","author":"HB Ong","year":"2013","journal-title":"Mol Microbiol"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1111\/j.1365-2958.2011.07563.x","article-title":"Requirement for acetyl-CoA carboxylase in Trypanosoma brucei is dependent upon the growth environment","volume":"80","author":"PA Vigueira","year":"2011","journal-title":"Mol Microbiol"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"23636","DOI":"10.1074\/jbc.M803600200","article-title":"Phosphatidylethanolamine in Trypanosoma brucei is organized in two separate pools and is synthesized exclusively by the Kennedy pathway","volume":"283","author":"A Signorell","year":"2008","journal-title":"J Biol Chem"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1111\/j.1747-0285.2011.01156.x","article-title":"High-throughput analysis of an RNAi library identifies novel kinase targets in Trypanosoma brucei","volume":"78","author":"ZB Mackey","year":"2011","journal-title":"Chem Biol Drug Des"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1101\/gr.115089.110","article-title":"High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome","volume":"21","author":"S Alsford","year":"2011","journal-title":"Genome Res"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1002\/wrna.110","article-title":"RNAi screening: new approaches, understandings, and organisms","volume":"3","author":"SE Mohr","year":"2012","journal-title":"Wiley Interdiscip Rev RNA"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"e1003886","DOI":"10.1371\/journal.ppat.1003886","article-title":"Regulators of Trypanosoma brucei cell cycle progression and differentiation identified using a kinome-wide RNAi screen","volume":"10","author":"NG Jones","year":"2014","journal-title":"PLoS Pathog"}],"container-title":["PLoS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0003430","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T11:48:06Z","timestamp":1650973686000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0003430"}},"subtitle":[],"editor":[{"given":"Michael P.","family":"Pollastri","sequence":"first","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2015,1,8]]},"references-count":58,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2015,1,8]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0003430","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,1,8]]}}}