{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T10:59:31Z","timestamp":1761217171817},"reference-count":23,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2008,3,19]],"date-time":"2008-03-19T00:00:00Z","timestamp":1205884800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0001823","type":"journal-article","created":{"date-parts":[[2008,3,18]],"date-time":"2008-03-18T15:20:48Z","timestamp":1205853648000},"page":"e1823","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["A Dual-Color Fluorescence-Based Platform to Identify Selective Inhibitors of Akt Signaling"],"prefix":"10.1371","volume":"3","author":[{"given":"Aranzaz\u00fa","family":"Rosado","sequence":"first","affiliation":[]},{"given":"Fabian","family":"Zanella","sequence":"additional","affiliation":[]},{"given":"Beatriz","family":"Garcia","sequence":"additional","affiliation":[]},{"given":"Amancio","family":"Carnero","sequence":"additional","affiliation":[]},{"given":"Wolfgang","family":"Link","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2008,3,19]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1960","DOI":"10.1126\/science.287.5460.1960","article-title":"Drug discovery: a historical perspective.","volume":"287","author":"J Drews","year":"2000","journal-title":"Science"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1093\/jnci\/94.2.78","article-title":"Evolution of anticancer drug discovery and the role of cell-based screening.","volume":"94","author":"FM Balis","year":"2002","journal-title":"J Natl Cancer Inst"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1969","DOI":"10.1126\/science.287.5460.1969","article-title":"Mechanism-based target identification and drug discovery in cancer research.","volume":"287","author":"JB Gibbs","year":"2000","journal-title":"Science"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/S0092-8674(85)80053-2","article-title":"Il-3-dependent mouse clones that express B-220 surface antigen, contain Ig genes in germ-line configuration, and generate B lymphocytes in vivo.","volume":"41","author":"R Palacios","year":"1985","journal-title":"Cell"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S0092-8674(00)81013-2","article-title":"Fetal anemia and apoptosis of red cell progenitors in Stat5a-\/-5b-\/- mice: a direct role for Stat5 in Bcl-X(L) induction.","volume":"98","author":"M Socolovsky","year":"1999","journal-title":"Cell"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"4181","DOI":"10.1002\/j.1460-2075.1993.tb06102.x","article-title":"Signal transduction by the high-affinity GM-CSF receptor: two distinct cytoplasmic regions of the common beta subunit responsible for different signaling.","volume":"12","author":"N Sato","year":"1993","journal-title":"Embo J"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"11345","DOI":"10.1073\/pnas.94.21.11345","article-title":"Interleukin 3-dependent survival by the Akt protein kinase.","volume":"94","author":"Z Songyang","year":"1997","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1016\/j.molcel.2006.02.009","article-title":"Glycogen synthase kinase-3 regulates mitochondrial outer membrane permeabilization and apoptosis by destabilization of MCL-1.","volume":"21","author":"U Maurer","year":"2006","journal-title":"Mol Cell"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"5826","DOI":"10.1038\/sj.onc.1204549","article-title":"Cooperative and redundant effects of STAT5 and Ras signaling in BCR\/ABL transformed hematopoietic cells.","volume":"20","author":"RR Hoover","year":"2001","journal-title":"Oncogene"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/sj.cdd.4400492","article-title":"Role of PI3-kinase in Bcl-X induction and apoptosis inhibition mediated by IL-3 or IGF-1 in Baf-3 cells.","volume":"6","author":"Y Leverrier","year":"1999","journal-title":"Cell Death Differ"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"3871","DOI":"10.1128\/MCB.18.7.3871","article-title":"Identification and characterization of a constitutively active STAT5 mutant that promotes cell proliferation.","volume":"18","author":"M Onishi","year":"1998","journal-title":"Molecular and Cellular Biology"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1038\/376599a0","article-title":"Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction.","volume":"376","author":"BM Burgering","year":"1995","journal-title":"Nature"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"4754","DOI":"10.1093\/emboj\/18.17.4754","article-title":"STAT5 as a molecular regulator of proliferation, differentiation and apoptosis in hematopoietic cells.","volume":"18","author":"T Nosaka","year":"1999","journal-title":"Embo J"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1146\/annurev.biochem.68.1.965","article-title":"AKT\/PKB and other D3 phosphoinositide-regulated kinases: kinase activation by phosphoinositide-dependent phosphorylation.","volume":"68","author":"TO Chan","year":"1999","journal-title":"Annu Rev Biochem"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1089\/adt.2007.058","article-title":"An HTS approach to screen for antagonists of the nuclear export machinery using high content cell-based assays.","volume":"5","author":"F Zanella","year":"2007","journal-title":"Assay Drug Dev Technol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1042\/bj20031119","article-title":"Structural basis for UCN-01 (7-hydroxystaurosporine) specificity and PDK1 (3-phosphoinositide-dependent protein kinase-1) inhibition.","volume":"375","author":"D Komander","year":"2003","journal-title":"Biochem J"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"31924","DOI":"10.1074\/jbc.M507057200","article-title":"Identification of N10-substituted phenoxazines as potent and specific inhibitors of Akt signaling.","volume":"280","author":"KN Thimmaiah","year":"2005","journal-title":"J Biol Chem"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1038\/nbt1001-940","article-title":"Use of isogenic human cancer cells for high-throughput screening and drug discovery.","volume":"19","author":"CJ Torrance","year":"2001","journal-title":"Nat Biotechnol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1126\/science.278.5338.687","article-title":"Interleukin-3-induced phosphorylation of BAD through the protein kinase Akt.","volume":"278","author":"L del Peso","year":"1997","journal-title":"Science"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"12361","DOI":"10.1074\/jbc.M213069200","article-title":"Akt activation promotes degradation of tuberin and FOXO3a via the proteasome.","volume":"278","author":"DR Plas","year":"2003","journal-title":"J Biol Chem"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"2234","DOI":"10.1093\/nar\/28.11.2234","article-title":"Loss-of-function genetics in mammalian cells: the p53 tumor suppressor model.","volume":"28","author":"A Carnero","year":"2000","journal-title":"Nucleic Acids Res"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1002\/jcb.20479","article-title":"Membrane localization of all class I PI 3-kinase isoforms suppresses c-Myc-induced apoptosis in Rat1 fibroblasts via Akt.","volume":"95","author":"W Link","year":"2005","journal-title":"J Cell Biochem"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1177\/108705719900400206","article-title":"A simple statistical parameter for use in evaluation and validation of high throughput screening assays.","volume":"4","author":"JH Zhang","year":"1999","journal-title":"Journal of Biomolecular Screening"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0001823","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T02:34:41Z","timestamp":1557369281000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0001823"}},"subtitle":[],"editor":[{"given":"Mark R.","family":"Cookson","sequence":"first","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2008,3,19]]},"references-count":23,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2008,3,19]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0001823","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,3,19]]}}}