{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T21:18:55Z","timestamp":1760908735365},"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2011,9,19]],"date-time":"2011-09-19T00:00:00Z","timestamp":1316390400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0024927","type":"journal-article","created":{"date-parts":[[2011,9,19]],"date-time":"2011-09-19T21:23:21Z","timestamp":1316467401000},"page":"e24927","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":20,"title":["Human NK Cells Differ More in Their KIR2DL1-Dependent Thresholds for HLA-Cw6-Mediated Inhibition than in Their Maximal Killing Capacity"],"prefix":"10.1371","volume":"6","author":[{"given":"Catarina R.","family":"Almeida","sequence":"first","affiliation":[]},{"given":"Amit","family":"Ashkenazi","sequence":"additional","affiliation":[]},{"given":"Gitit","family":"Shahaf","sequence":"additional","affiliation":[]},{"given":"Deborah","family":"Kaplan","sequence":"additional","affiliation":[]},{"given":"Daniel M.","family":"Davis","sequence":"additional","affiliation":[]},{"given":"Ramit","family":"Mehr","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2011,9,19]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"6904","DOI":"10.4049\/jimmunol.177.10.6904","article-title":"Segregation of HLA-C from ICAM-1 at NK cell immune synapses is controlled by its cell surface density.","volume":"177","author":"CR Almeida","year":"2006","journal-title":"J Immunol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1538","DOI":"10.4049\/jimmunol.1000951","article-title":"A human NK cell activation\/inhibition threshold allows small changes in the target cell surface phenotype to dramatically alter susceptibility to NK cells.","volume":"186","author":"TD Holmes","year":"2011","journal-title":"J Immunol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"5606","DOI":"10.4049\/jimmunol.178.9.5606","article-title":"Inhibitory receptor signals suppress ligation-induced recruitment of NKG2D to GM1-rich membrane domains at the human NK cell immune synapse.","volume":"178","author":"J Endt","year":"2007","journal-title":"J Immunol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/nature09914","article-title":"Differential microRNA regulation of HLA-C expression and its association with HIV control.","volume":"472","author":"S Kulkarni","year":"2011","journal-title":"Nature"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"5977","DOI":"10.4049\/jimmunol.179.9.5977","article-title":"Hierarchy of the human natural killer cell response is determined by class and quantity of inhibitory receptors for Self-HLA-B and HLA-C ligands.","volume":"179","author":"J Yu","year":"2007","journal-title":"J Immunol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"2434","DOI":"10.1182\/blood-2008-05-156836","article-title":"The strength of inhibitory input during education quantitatively tunes the functional responsiveness of individual natural killer cells.","volume":"113","author":"P Brodin","year":"2009","journal-title":"Blood"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.immuni.2006.06.013","article-title":"Human NK cell education by inhibitory receptors for MHC class I.","volume":"25","author":"N Anfossi","year":"2006","journal-title":"Immunity"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"4572","DOI":"10.4049\/jimmunol.0803900","article-title":"NK Cell Responsiveness Is Tuned Commensurate with the Number of Inhibitory Receptors for Self-MHC Class I: The Rheostat Model.","volume":"182","author":"NT Joncker","year":"2009","journal-title":"J Immunol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1073\/pnas.92.5.1649","article-title":"Altered phenotype and function of natural killer cells expressing the major histocompatibility complex receptor Ly-49 in mice transgenic for its ligand.","volume":"92","author":"MY Olsson","year":"1995","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"3189","DOI":"10.4049\/jimmunol.159.7.3189","article-title":"NK cell receptor calibration: effects of MHC class I induction on killing by Ly49Ahigh and Ly49Alow NK cells.","volume":"159","author":"MY Olsson-Alheim","year":"1997","journal-title":"J Immunol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"760","DOI":"10.4049\/jimmunol.1002208","article-title":"Simulations of the NK Cell Immune Synapse Reveal that Activation Thresholds Can Be Established by Inhibitory Receptors Acting Locally.","volume":"187","author":"A Kaplan","year":"2011","journal-title":"J Immunol"},{"key":"ref12","first-page":"869","article-title":"Purified MHC class I molecules inhibit activated NK cells in a cell-free system in vitro.","volume":"31","author":"T Kambayashi","year":"2001","journal-title":"EurJImmunol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"3424","DOI":"10.4049\/jimmunol.0904057","article-title":"Effects of MHC class I alleles on licensing of Ly49A+ NK cells.","volume":"184","author":"AH Jonsson","year":"2010","journal-title":"J Immunol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"e13174","DOI":"10.1371\/journal.pone.0013174","article-title":"Natural Killer Cell Tolerance Persists Despite Significant Reduction of Self MHC Class I on Normal Target Cells in Mice.","volume":"5","author":"P Brodin","year":"2010","journal-title":"PLoS ONE"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"3053","DOI":"10.1073\/pnas.0712229105","article-title":"HLA alleles determine differences in human natural killer cell responsiveness and potency.","volume":"105","author":"S Kim","year":"2008","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1038\/bmt.2008.398","article-title":"Cytolytic activity of NK cell clones against acute childhood precursor-B-cell leukaemia is influenced by HLA class I expression on blasts and the differential KIR phenotype of NK clones.","volume":"43","author":"T Feuchtinger","year":"2009","journal-title":"Bone Marrow Transplant"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1084\/jem.20050167","article-title":"Natural killer cell education in mice with single or multiple major histocompatibility complex class I molecules.","volume":"201","author":"S Johansson","year":"2005","journal-title":"J Exp Med"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"3320","DOI":"10.4049\/jimmunol.142.9.3320","article-title":"Production of human cells expressing individual transferred HLA-A,-B,-C genes using an HLA-A,-B,-C null human cell line.","volume":"142","author":"Y Shimizu","year":"1989","journal-title":"J Immunol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1084\/jem.184.3.913","article-title":"Protection from lysis by natural killer cells of group 1 and 2 specificity is mediated by residue 80 in human histocompatibility leukocyte antigen C alleles and also occurs with empty major histocompatibility complex molecules.","volume":"184","author":"O Mandelboim","year":"1996","journal-title":"J Exp Med"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"14151","DOI":"10.1073\/pnas.1835830100","article-title":"The mature activating natural killer cell immunologic synapse is formed in distinct stages.","volume":"100","author":"JS Orange","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.4049\/jimmunol.173.3.1571","article-title":"Ligand binding to inhibitory killer cell Ig-like receptors induce colocalization with Src homology domain 2-containing protein tyrosine phosphatase 1 and interruption of ongoing activation signals 2876.","volume":"173","author":"YM Vyas","year":"2004","journal-title":"J Immunol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0022-1759(03)00265-5","article-title":"Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation.","volume":"281","author":"MR Betts","year":"2003","journal-title":"J Immunol Methods"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.jim.2004.08.008","article-title":"CD107a as a functional marker for the identification of natural killer cell activity.","volume":"294","author":"G Alter","year":"2004","journal-title":"J Immunol Methods"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1182\/blood-2009-08-238469","article-title":"Regulation of human NK-cell cytokine and chemokine production by target cell recognition.","volume":"115","author":"C Fauriat","year":"2010","journal-title":"Blood"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"3655","DOI":"10.4049\/jimmunol.179.6.3655","article-title":"Intracellular cysteine residues in the tail of MHC class I proteins are crucial for extracellular recognition by leukocyte Ig-like receptor 1.","volume":"179","author":"R Gruda","year":"2007","journal-title":"J Immunol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1084\/jem.187.5.813","article-title":"Recognition of human histocompatibility leukocyte antigen (HLA)-E complexed with HLA class I signal sequence-derived peptides by CD94\/NKG2 confers protection from natural killer cell-mediated lysis.","volume":"187","author":"F Borrego","year":"1998","journal-title":"J Exp Med"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1038\/35869","article-title":"HLA-E binds to natural killer cell receptors CD94\/NKG2A, B and C.","volume":"391","author":"VM Braud","year":"1998","journal-title":"Nature"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"5199","DOI":"10.1073\/pnas.95.9.5199","article-title":"HLA-E is a major ligand for the natural killer inhibitory receptor CD94\/NKG2A.","volume":"95","author":"N Lee","year":"1998","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1046\/j.1440-1711.1999.00865.x","article-title":"Using carboxyfluorescein diacetate succinimidyl ester to monitor human NK cell division: analysis of the effect of activating and inhibitory class I MHC receptors.","volume":"77","author":"HS Warren","year":"1999","journal-title":"Immunol Cell Biol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"e326","DOI":"10.1371\/journal.pone.0000326","article-title":"Serial killing of tumor cells by human natural killer cells - enhancement by therapeutic antibodies.","volume":"2","author":"R Bhat","year":"2007","journal-title":"PLoS One"},{"key":"ref31","first-page":"6","article-title":"Self-tolerance, dendritic cell (DC)-mediated activation and tissue distribution of natural killer (NK) cells.","volume":"110","author":"I Zanoni","year":"2007","journal-title":"ImmunolLett"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"6488","DOI":"10.4049\/jimmunol.163.12.6488","article-title":"IFN-gamma production and cytotoxicity of IL-2-activated murine NK cells are differentially regulated by MHC class I molecules.","volume":"163","author":"A Kubota","year":"1999","journal-title":"JImmunol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.it.2009.01.006","article-title":"NK cell education: not an on-off switch but a tunable rheostat.","volume":"30","author":"P Brodin","year":"2009","journal-title":"Trends Immunol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"e6046","DOI":"10.1371\/journal.pone.0006046","article-title":"Probing natural killer cell education by Ly49 receptor expression analysis and computational modelling in single MHC class I mice.","volume":"4","author":"S Johansson","year":"2009","journal-title":"PLoS One"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1126\/science.1068440","article-title":"Effectiveness of donor natural killer cell alloreactivity in mismatched hematopoietic transplants.","volume":"295","author":"L Ruggeri","year":"2002","journal-title":"Science"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1111\/j.1600-0854.2007.00603.x","article-title":"Membranous structures transfer cell surface proteins across NK cell immune synapses.","volume":"8","author":"GS Williams","year":"2007","journal-title":"Traffic"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.humimm.2004.01.004","article-title":"Regulation of FasL expression in natural killer cells.","volume":"65","author":"HL Chua","year":"2004","journal-title":"Hum Immunol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1016\/j.molimm.2004.07.033","article-title":"Impact of FASL-induced apoptosis in the elimination of tumor cells by NK cells.","volume":"42","author":"V Screpanti","year":"2005","journal-title":"Mol Immunol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0024927","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T19:17:57Z","timestamp":1686338277000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0024927"}},"subtitle":[],"editor":[{"given":"Jacques","family":"Zimmer","sequence":"first","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2011,9,19]]},"references-count":38,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2011,9,19]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0024927","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,19]]}}}