{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T10:09:16Z","timestamp":1760522956225},"reference-count":24,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2012,2,29]],"date-time":"2012-02-29T00:00:00Z","timestamp":1330473600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0032646","type":"journal-article","created":{"date-parts":[[2012,2,29]],"date-time":"2012-02-29T22:32:31Z","timestamp":1330554751000},"page":"e32646","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["A Rapid FACS-Based Strategy to Isolate Human Gene Knockin and Knockout Clones"],"prefix":"10.1371","volume":"7","author":[{"given":"Jo\u00e3o F.","family":"Mata","sequence":"first","affiliation":[]},{"given":"Telma","family":"Lopes","sequence":"additional","affiliation":[]},{"given":"Rui","family":"Gardner","sequence":"additional","affiliation":[]},{"given":"Lars E. T.","family":"Jansen","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2012,2,29]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"3365","DOI":"10.1128\/MCB.12.8.3365","article-title":"Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus.","volume":"12","author":"C Deng","year":"1992","journal-title":"Mol Cell Biol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1038\/nprot.2011.301","article-title":"AAV-mediated gene targeting methods for human cells.","volume":"6","author":"IF Khan","year":"2011","journal-title":"Nat Protoc"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1038\/nbt1353","article-title":"Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery.","volume":"25","author":"A Lombardo","year":"2007","journal-title":"Nat Biotechnol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1038\/nature03556","article-title":"Highly efficient endogenous human gene correction using designed zinc-finger nucleases.","volume":"435","author":"FD Urnov","year":"2005","journal-title":"Nature"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1038\/nbt.1562","article-title":"Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases.","volume":"27","author":"D Hockemeyer","year":"2009","journal-title":"Nat Biotechnol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1038\/mt.2008.114","article-title":"Zinc-finger nucleases: the next generation emerges.","volume":"16","author":"T Cathomen","year":"2008","journal-title":"Mol Ther"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1038\/nbt0702-735","article-title":"Targeted transgene insertion into human chromosomes by adeno-associated virus vectors.","volume":"20","author":"R Hirata","year":"2002","journal-title":"Nat Biotechnol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1038\/ng0498-325","article-title":"Human gene targeting by viral vectors.","volume":"18","author":"DW Russell","year":"1998","journal-title":"Nat Genet"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"e3","DOI":"10.1093\/nar\/gnh009","article-title":"Facile methods for generating human somatic cell gene knockouts using recombinant adeno-associated viruses.","volume":"32","author":"M Kohli","year":"2004","journal-title":"Nucleic Acids Res"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1038\/mt.2010.55","article-title":"Engineering of human pluripotent stem cells by AAV-mediated gene targeting.","volume":"18","author":"IF Khan","year":"2010","journal-title":"Mol Ther"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1126\/science.1124618","article-title":"The fluorescent toolbox for assessing protein location and function.","volume":"312","author":"BNG Giepmans","year":"2006","journal-title":"Science"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1126\/science.1181348","article-title":"Systematic analysis of human protein complexes identifies chromosome segregation proteins.","volume":"328","author":"JRA Hutchins","year":"2010","journal-title":"Science"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.ymeth.2010.09.003","article-title":"Recombineering BAC transgenes for protein tagging.","volume":"53","author":"G Ciotta","year":"2011","journal-title":"Methods"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/978-1-60327-993-2_2","article-title":"Functional dissection of mitotic regulators through gene targeting in human somatic cells.","volume":"545","author":"E Berdougo","year":"2009","journal-title":"Methods Mol Biol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"pl1","DOI":"10.1126\/stke.2662005pl1","article-title":"A combined approach for the localization and tandem affinity purification of protein complexes from metazoans.","volume":"2005","author":"IM Cheeseman","year":"2005","journal-title":"Sci STKE"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1083\/jcb.127.3.581","article-title":"Human CENP-A contains a histone H3 related histone fold domain that is required for targeting to the centromere.","volume":"127","author":"KF Sullivan","year":"1994","journal-title":"J Cell Biol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1083\/jcb.136.3.501","article-title":"Assembly of CENP-A into centromeric chromatin requires a cooperative array of nucleosomal DNA contact sites.","volume":"136","author":"RD Shelby","year":"1997","journal-title":"J Cell Biol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.molcel.2006.12.018","article-title":"Centromere identity maintained by nucleosomes assembled with histone H3 containing the CENP-A targeting domain.","volume":"25","author":"BE Black","year":"2007","journal-title":"Mol Cell"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/S0968-0004(98)01208-0","article-title":"A rule for termination-codon position within intron-containing genes: when nonsense affects RNA abundance.","volume":"23","author":"E Nagy","year":"1998","journal-title":"Trends Biochem Sci"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1089\/hum.2008.061","article-title":"Human gene targeting favors insertions over deletions.","volume":"19","author":"DW Russell","year":"2008","journal-title":"Hum Gene Ther"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"16357","DOI":"10.1073\/pnas.0508263102","article-title":"Isolating gene-corrected stem cells without drug selection.","volume":"102","author":"S Hatada","year":"2005","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref22","first-page":"84","article-title":"Development of human gene reporter cell lines using rAAV mediated homologous recombination.","volume":"9","author":"SL Fernandez","year":"2007","journal-title":"Biol Proced Online"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"5128","DOI":"10.1073\/pnas.89.11.5128","article-title":"Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs.","volume":"89","author":"H te Riele","year":"1992","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1083\/jcb.200701066","article-title":"Propagation of centromeric chromatin requires exit from mitosis.","volume":"176","author":"LET Jansen","year":"2007","journal-title":"J Cell Biol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0032646","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,20]],"date-time":"2024-04-20T22:10:10Z","timestamp":1713651010000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0032646"}},"subtitle":[],"editor":[{"given":"Beth A.","family":"Sullivan","sequence":"first","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2012,2,29]]},"references-count":24,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2012,2,29]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0032646","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2,29]]}}}