{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T16:02:27Z","timestamp":1775750547767,"version":"3.50.1"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:00:00Z","timestamp":1339459200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0038559","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T21:02:15Z","timestamp":1339534935000},"page":"e38559","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":47,"title":["Circulating DNA: Diagnostic Tool and Predictive Marker for Overall Survival of NSCLC Patients"],"prefix":"10.1371","volume":"7","author":[{"given":"Raquel","family":"Catarino","sequence":"first","affiliation":[]},{"given":"Ana","family":"Coelho","sequence":"additional","affiliation":[]},{"given":"Ant\u00f3nio","family":"Ara\u00fajo","sequence":"additional","affiliation":[]},{"given":"M\u00f3nica","family":"Gomes","sequence":"additional","affiliation":[]},{"given":"Augusto","family":"Nogueira","sequence":"additional","affiliation":[]},{"given":"Carlos","family":"Lopes","sequence":"additional","affiliation":[]},{"given":"Rui","family":"Medeiros","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2012,6,12]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.lungcan.2008.07.012","article-title":"Circulating plasma DNA as diagnostic biomarker in non-small cell lung cancer.","volume":"64","author":"M Paci","year":"2009","journal-title":"Lung Cancer"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1136\/thorax.57.11.992","article-title":"Lung cancer. Introduction.","volume":"57","author":"T Sethi","year":"2002","journal-title":"Thorax"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1378\/chest.122.3.1037","article-title":"Prognostic factors in non-small cell lung cancer: a decade of progress.","volume":"122","author":"MD Brundage","year":"2002","journal-title":"Chest"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"2208","DOI":"10.1002\/cncr.21869","article-title":"Changes in the natural history of nonsmall cell lung cancer (NSCLC)\u2013comparison of outcomes and characteristics in patients with advanced NSCLC entered in Eastern Cooperative Oncology Group trials before and after 1990.","volume":"106","author":"HA Wakelee","year":"2006","journal-title":"Cancer"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1002\/cncr.22979","article-title":"Predictive and prognostic value of FDG-PET in nonsmall-cell lung cancer: a systematic review.","volume":"110","author":"LF de Geus-Oei","year":"2007","journal-title":"Cancer"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1016\/S0140-6736(05)67569-1","article-title":"Small-cell lung cancer.","volume":"366","author":"DM Jackman","year":"2005","journal-title":"Lancet"},{"key":"ref7","first-page":"181","article-title":"Circulating nucleic acids (CNAs) and cancer\u2013a survey.","volume":"1775","author":"M Fleischhacker","year":"2007","journal-title":"Biochim Biophys Acta"},{"key":"ref8","first-page":"241","article-title":"Les acides nucl\u00e9iques du plasma sanguin chez l\u2019homme.","volume":"142","author":"P Mandel","year":"1948","journal-title":"CR Acad Sci Paris"},{"key":"ref9","first-page":"67","article-title":"Detection of aberrant promoter hypermethylation of tumor suppressor genes in serum DNA from non-small cell lung cancer patients.","volume":"59","author":"M Esteller","year":"1999","journal-title":"Cancer Res"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1023\/A:1006260319913","article-title":"Detection of circulating tumour DNA in the blood (plasma\/serum) of cancer patients.","volume":"18","author":"P Anker","year":"1999","journal-title":"Cancer Metastasis Rev"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"4157","DOI":"10.1200\/JCO.2004.11.123","article-title":"Circulating deoxyribonucleic Acid as prognostic marker in non-small-cell lung cancer patients undergoing chemotherapy.","volume":"22","author":"O Gautschi","year":"2004","journal-title":"J Clin Oncol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.mrrev.2006.11.002","article-title":"Circulating free DNA in plasma or serum as biomarker of carcinogenesis: practical aspects and biological significance.","volume":"635","author":"E Gormally","year":"2007","journal-title":"Mutat Res"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"3902","DOI":"10.1200\/JCO.2003.02.006","article-title":"Quantification of free circulating DNA as a diagnostic marker in lung cancer.","volume":"21","author":"G Sozzi","year":"2003","journal-title":"J Clin Oncol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.lungcan.2009.11.009","article-title":"Value of quantitative analysis of circulating cell free DNA as a screening tool for lung cancer: a meta-analysis.","volume":"69","author":"R Zhang","year":"2010","journal-title":"Lung Cancer"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"3891","DOI":"10.1200\/JCO.2003.07.976","article-title":"Early detection of lung cancer using serum RNA or DNA markers: ready for \u201cprime time\u201d or for validation?","volume":"21","author":"PJ Bunn Jr","year":"2003","journal-title":"J Clin Oncol"},{"key":"ref16","first-page":"4675","article-title":"Analysis of circulating tumor DNA in plasma at diagnosis and during follow-up of lung cancer patients.","volume":"61","author":"G Sozzi","year":"2001","journal-title":"Cancer Res"},{"key":"ref17","first-page":"455","article-title":"Prognostic value of hTERT mRNA expression in surgical samples of lung cancer patients: the European Early Lung Cancer Project.","volume":"37","author":"RM Van den Berg","year":"2010","journal-title":"International journal of oncology"},{"key":"ref18","first-page":"1157","article-title":"Increased human telomerase reverse transcriptase (hTERT) mRNA expression but not telomerase activity is related to survival in curatively resected non-small cell lung cancer.","volume":"29","author":"R Metzger","year":"2009","journal-title":"Anticancer research"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1101\/gr.6.10.986","article-title":"Real time quantitative PCR.","volume":"6","author":"CA Heid","year":"1996","journal-title":"Genome Res"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.bbrc.2008.03.039","article-title":"Oncogenic virus-associated neoplasia: a role for cyclin D1 genotypes influencing the age of onset of disease?","volume":"370","author":"R Catarino","year":"2008","journal-title":"Biochemical and biophysical research communications"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.lungcan.2004.12.008","article-title":"Circulating tumour-derived DNA and RNA markers in blood: a tool for early detection, diagnostics, and follow-up?","volume":"49","author":"RM Bremnes","year":"2005","journal-title":"Lung Cancer"},{"key":"ref22","first-page":"646","article-title":"Free DNA in the serum of cancer patients and the effect of therapy.","volume":"37","author":"SA Leon","year":"1977","journal-title":"Cancer Res"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1848","DOI":"10.1093\/jnci\/dji432","article-title":"Effects of prolonged storage of whole plasma or isolated plasma DNA on the results of circulating DNA quantification assays.","volume":"97","author":"G Sozzi","year":"2005","journal-title":"J Natl Cancer Inst"},{"key":"ref24","first-page":"3761","article-title":"Tumor DNA in plasma at diagnosis of breast cancer patients is a valuable predictor of disease-free survival.","volume":"8","author":"JM Silva","year":"2002","journal-title":"Clin Cancer Res"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.lungcan.2009.06.021","article-title":"Circulating DNA is a non-invasive prognostic factor for survival in non-small cell lung cancer.","volume":"68","author":"MA van der Drift","year":"2010","journal-title":"Lung Cancer"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1164\/rccm.200807-1068OC","article-title":"Plasma DNA quantification in lung cancer computed tomography screening: five-year results of a prospective study.","volume":"179","author":"G Sozzi","year":"2009","journal-title":"Am J Respir Crit Care Med"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0305-7372(02)00077-4","article-title":"Circulating DNA: a new diagnostic gold mine?","volume":"28","author":"A Ziegler","year":"2002","journal-title":"Cancer Treat Rev"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1002\/ijc.11079","article-title":"Plasma DNA microsatellite panel as sensitive and tumor-specific marker in lung cancer patients.","volume":"105","author":"M Beau-Faller","year":"2003","journal-title":"Int J Cancer"},{"key":"ref29","first-page":"1485","article-title":"Quantification of plasma DNA as a screening tool for lung cancer.","volume":"117","author":"GS Xie","year":"2004","journal-title":"Chin Med J (Engl)"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1373\/clinchem.2004.039263","article-title":"Quantitative analysis of circulating plasma DNA as a tumor marker in thoracic malignancies.","volume":"51","author":"LJ Herrera","year":"2005","journal-title":"Clin Chem"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1038\/sj.bjc.6601609","article-title":"Quantitation of circulating DNA in the serum of breast cancer patients by real-time PCR.","volume":"90","author":"S Gal","year":"2004","journal-title":"Br J Cancer"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.canlet.2005.11.027","article-title":"Quantitative analysis of plasma circulating DNA at diagnosis and during follow-up of breast cancer patients.","volume":"243","author":"ZH Huang","year":"2006","journal-title":"Cancer Lett"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1492","DOI":"10.1038\/sj.bjc.6603117","article-title":"Cell-free and cell-bound circulating DNA in breast tumours: DNA quantification and analysis of tumour-related gene methylation.","volume":"94","author":"TE Skvortsova","year":"2006","journal-title":"Br J Cancer"},{"key":"ref34","first-page":"184","article-title":"Quantification of Free Circulating Tumor DNA in Plasma as a Diagnostic Marker in Breast Cancer.","volume":"5\/4","author":"R Catarino","year":"2007","journal-title":"Eur J Cancer Supplements"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1081\/NCN-200026035","article-title":"Extracellular circulating nucleic acids in human plasma in health and disease.","volume":"23","author":"PP Laktionov","year":"2004","journal-title":"Nucleosides Nucleotides Nucleic Acids"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1093\/clinchem\/48.8.1186","article-title":"Plasma nucleic acids in the diagnosis and management of malignant disease.","volume":"48","author":"PJ Johnson","year":"2002","journal-title":"Clin Chem"},{"key":"ref37","first-page":"1833","article-title":"Circulating cell-free DNA in plasma\/serum of lung cancer patients as a potential screening and prognostic tool.","volume":"52","author":"AK Pathak","year":"2006","journal-title":"Clin Chem"},{"key":"ref38","doi-asserted-by":"crossref","DOI":"10.1200\/JCO.2005.05.430","article-title":"Reproducibility of a semiquantitative measurement of circulating DNA in plasma from neoplastic patients.","author":"M Frattini","year":"2005"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1136\/gut.50.4.530","article-title":"Detection of epithelial tumour RNA in the plasma of colon cancer patients is associated with advanced stages and circulating tumour cells.","volume":"50","author":"JM Silva","year":"2002","journal-title":"Gut"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1086\/302205","article-title":"Rapid clearance of fetal DNA from maternal plasma.","volume":"64","author":"YM Lo","year":"1999","journal-title":"Am J Hum Genet"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"16368","DOI":"10.1073\/pnas.0507904102","article-title":"Detection and quantification of mutations in the plasma of patients with colorectal tumors.","volume":"102","author":"F Diehl","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref42","first-page":"1634","article-title":"Detection of oncogene mutations in sputum precedes diagnosis of lung cancer.","volume":"54","author":"L Mao","year":"1994","journal-title":"Cancer Res"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0038559","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T00:50:46Z","timestamp":1594255846000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0038559"}},"subtitle":[],"editor":[{"given":"Chulso","family":"Moon","sequence":"first","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2012,6,12]]},"references-count":42,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2012,6,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0038559","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6,12]]}}}