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In: Bergmeyer HU, Bergmeyer J, Grabl M, editors. Methods of enzymatic analysis. Verlag-Chemie, Weinheim, Germany: Vol. VII, 566\u2013571."},{"key":"ref52","doi-asserted-by":"crossref","first-page":"5924","DOI":"10.1111\/j.1742-4658.2007.06115.x","article-title":"The lactate dehydrogenases encoded by the <italic>ldh<\/italic> and <italic>ldhB<\/italic> genes in <italic>Lactococcus lactis<\/italic> exhibit distinct regulation and catalytic properties - comparative modeling to probe the molecular basis","volume":"274","author":"P Gaspar","year":"2007","journal-title":"FEBS J"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1128\/JB.158.3.1188-1190.1984","article-title":"Attachment of pneumococcal autolysin to wall teichoic acids, an essential step in enzymatic wall degradation","volume":"158","author":"S Giudicelli","year":"1984","journal-title":"J Bacteriol"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"3108","DOI":"10.1128\/JB.183.10.3108-3116.2001","article-title":"The autolytic enzyme LytA of <italic>Streptococcus pneumoniae<\/italic> is not responsible for releasing pneumolysin","volume":"183","author":"P Balachandran","year":"2001","journal-title":"J Bacteriol"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"7681","DOI":"10.1073\/pnas.112464599","article-title":"Induction of natural competence in <italic>Streptococcus pneumoniae<\/italic> triggers lysis and DNA release from a subfraction of the cell population","volume":"99","author":"H Steinmoen","year":"2002","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0058492","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T13:11:26Z","timestamp":1644498686000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0058492"}},"subtitle":[],"editor":[{"given":"Malcolm James","family":"Horsburgh","sequence":"first","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2013,3,7]]},"references-count":55,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2013,3,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0058492","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3,7]]}}}