{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T14:29:15Z","timestamp":1768400955801,"version":"3.49.0"},"reference-count":40,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2013,3,19]],"date-time":"2013-03-19T00:00:00Z","timestamp":1363651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0059928","type":"journal-article","created":{"date-parts":[[2013,3,19]],"date-time":"2013-03-19T21:06:03Z","timestamp":1363727163000},"page":"e59928","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":59,"title":["Generation and Characterization of a Novel Mouse Embryonic Stem Cell Line with a Dynamic Reporter of Nanog Expression"],"prefix":"10.1371","volume":"8","author":[{"given":"Elsa","family":"Abranches","sequence":"first","affiliation":[]},{"given":"Evguenia","family":"Bekman","sequence":"additional","affiliation":[]},{"given":"Domingos","family":"Henrique","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2013,3,19]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1242\/dev.110.4.1001","article-title":"Stem cells: attributes, cycles, spirals, pitfalls and uncertainties. Lessons for and from the crypt","volume":"110","author":"CS Potten","year":"1990","journal-title":"Development"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/S0092-8674(00)81867-X","article-title":"Regulatory mechanisms in stem cell biology","volume":"88","author":"SJ Morrison","year":"1997","journal-title":"Cell"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"7634","DOI":"10.1073\/pnas.78.12.7634","article-title":"Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells","volume":"78","author":"GR Martin","year":"1981","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1038\/292154a0","article-title":"Establishment in culture of pluripotential cells from mouse embryos","volume":"292","author":"MJ Evans","year":"1981","journal-title":"Nature"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1146\/annurev.cellbio.17.1.435","article-title":"Embryo-derived stem cells: of mice and men","volume":"17","author":"AG Smith","year":"2001","journal-title":"Annu Rev Cell Dev Biol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1038\/nature06968","article-title":"The ground state of embryonic stem cell self-renewal","volume":"453","author":"Q-L Ying","year":"2008","journal-title":"Nature"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1016\/j.cell.2008.02.006","article-title":"Capturing pluripotency","volume":"132","author":"J Silva","year":"2008","journal-title":"Cell"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1242\/dev.02787","article-title":"How is pluripotency determined and maintained?","volume":"134","author":"H Niwa","year":"2007","journal-title":"Development"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1016\/j.cell.2011.01.032","article-title":"Control of the embryonic stem cell state","volume":"144","author":"RA Young","year":"2011","journal-title":"Cell"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/j.cell.2008.01.015","article-title":"Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming","volume":"132","author":"R Jaenisch","year":"2008","journal-title":"Cell"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/j.cell.2011.05.017","article-title":"Pluripotency factors in embryonic stem cells regulate differentiation into germ layers","volume":"145","author":"M Thomson","year":"2011","journal-title":"Cell"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.stem.2011.03.013","article-title":"A precarious balance: pluripotency factors as lineage specifiers","volume":"8","author":"KM Loh","year":"2011","journal-title":"Cell Stem Cell"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/S0092-8674(01)00475-5","article-title":"Evidence for a pioneer round of mRNA translation: mRNAs subject to nonsense-mediated decay in mammalian cells are bound by CBP80 and CBP20","volume":"106","author":"Y Ishigaki","year":"2001","journal-title":"Cell"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1242\/dev.017400","article-title":"Identification and characterization of subpopulations in undifferentiated ES cell culture","volume":"135","author":"Y Toyooka","year":"2008","journal-title":"Development"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"2534","DOI":"10.1634\/stemcells.2007-0126","article-title":"A heterogeneous expression pattern for Nanog in embryonic stem cells","volume":"25","author":"AM Singh","year":"2007","journal-title":"Stem Cells"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.1038\/nature06403","article-title":"Nanog safeguards pluripotency and mediates germline development","volume":"450","author":"I Chambers","year":"2007","journal-title":"Nature"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"2230","DOI":"10.1098\/rstb.2011.0043","article-title":"Transcription factor heterogeneity and epiblast pluripotency","volume":"366","author":"R Osorno","year":"2011","journal-title":"Philosophical Transactions of the Royal Society B: Biological Sciences"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/S0092-8674(03)00393-3","article-title":"The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells","volume":"113","author":"K Mitsui","year":"2003","journal-title":"Cell"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1016\/S0092-8674(03)00392-1","article-title":"Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells","volume":"113","author":"I Chambers","year":"2003","journal-title":"Cell"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1038\/35093556","article-title":"Recombineering: a powerful new tool for mouse functional genomics","volume":"2","author":"NG Copeland","year":"2001","journal-title":"Nat Rev Genet"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/j.cell.2004.11.015","article-title":"Circadian gene expression in individual fibroblasts: cell-autonomous and self-sustained oscillators pass time to daughter cells","volume":"119","author":"E Nagoshi","year":"2004","journal-title":"Cell"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/1741-7007-9-58","article-title":"A novel reporter of notch signalling indicates regulated and random notch activation during vertebrate neurogenesis","volume":"9","author":"F Vilas-Boas","year":"2011","journal-title":"BMC Biol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"e6286","DOI":"10.1371\/journal.pone.0006286","article-title":"Neural differentiation of embryonic stem cells in vitro: a road map to neurogenesis in the embryo","volume":"4","author":"E Abranches","year":"2009","journal-title":"PLoS ONE"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"e1000149","DOI":"10.1371\/journal.pbio.1000149","article-title":"Regulated fluctuations in nanog expression mediate cell fate decisions in embryonic stem cells","volume":"7","author":"T Kalmar","year":"2009","journal-title":"PLoS Biol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"2895","DOI":"10.1242\/dev.02880","article-title":"FGF stimulation of the Erk1\/2 signalling cascade triggers transition of pluripotent embryonic stem cells from self-renewal to lineage commitment","volume":"134","author":"T Kunath","year":"2007","journal-title":"Development"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"2889","DOI":"10.1242\/dev.02858","article-title":"A discrete period of FGF-induced Erk1\/2 signalling is required for vertebrate neural specification","volume":"134","author":"MP Stavridis","year":"2007","journal-title":"Development"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1016\/S0960-9822(03)00381-6","article-title":"Negative feedback regulation of FGF signaling levels by Pyst1\/MKP3 in chick embryos","volume":"13","author":"MC Eblaghie","year":"2003","journal-title":"Curr Biol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1038\/ncb989","article-title":"MKP3 mediates the cellular response to FGF8 signalling in the vertebrate limb","volume":"5","author":"Y Kawakami","year":"2003","journal-title":"Nat Cell Biol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1242\/jcs.112.5.601","article-title":"Formation of a primitive ectoderm like cell population, EPL cells, from ES cells in response to biologically derived factors","volume":"5)","author":"J Rathjen","year":"1999","journal-title":"J Cell Sci 112 (Pt"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"229","DOI":"10.3390\/genes2010229","article-title":"The Function of E-Cadherin in Stem Cell Pluripotency and Self-Renewal","volume":"2","author":"F Soncin","year":"2011","journal-title":"Genes"},{"key":"ref31","doi-asserted-by":"crossref","unstructured":"Wang Y, Mah N, Prigione A, Wolfrum K, Andrade-Navarro MA, <etal>et al<\/etal>.. (2010) A Transcriptional Roadmap to the Induction of Pluripotency in Somatic Cells. Stem cell reviews.","DOI":"10.1007\/s12015-010-9137-2"},{"key":"ref32","doi-asserted-by":"crossref","unstructured":"Teo AKK, Arnold SJ, Trotter MWB, Brown S, Ang LT, <etal>et al<\/etal>.. (2011) Pluripotency factors regulate definitive endoderm specification through eomesodermin. Genes &amp; development.","DOI":"10.1101\/gad.607311"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"3081","DOI":"10.1242\/dev.021519","article-title":"Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst","volume":"135","author":"B Plusa","year":"2008","journal-title":"Development"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/j.devcel.2006.02.020","article-title":"Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway","volume":"10","author":"C Chazaud","year":"2006","journal-title":"Developmental Cell"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1042\/BST0381027","article-title":"The ground state of pluripotency","volume":"38","author":"J Wray","year":"2010","journal-title":"Biochem Soc Trans"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"e64","DOI":"10.1093\/nar\/gkm163","article-title":"A recombineering based approach for high-throughput conditional knockout targeting vector construction","volume":"35","author":"W Chan","year":"2007","journal-title":"Nucleic Acids Res"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR","volume":"29","author":"MW Pfaffl","year":"2001","journal-title":"Nucleic Acids Res"},{"key":"ref38","first-page":"67","article-title":"Determining protein half-lives","volume":"284","author":"P Zhou","year":"2004","journal-title":"Methods Mol Biol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"5328","DOI":"10.1073\/pnas.82.16.5328","article-title":"Actinomycin and DNA transcription","volume":"82","author":"HM Sobell","year":"1985","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref40","doi-asserted-by":"crossref","unstructured":"Akeson EC, Davisson MT (2001) Mitotic chromosome preparations from mouse cells for karyotyping. Curr Protoc Hum Genet Chapter 4: Unit4 10.","DOI":"10.1002\/0471142905.hg0410s25"}],"updated-by":[{"DOI":"10.1371\/journal.pone.0101028","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2014,6,20]],"date-time":"2014-06-20T00:00:00Z","timestamp":1403222400000}}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0059928","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,11]],"date-time":"2022-02-11T21:57:45Z","timestamp":1644616665000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0059928"}},"subtitle":[],"editor":[{"given":"Qiang","family":"Wu","sequence":"first","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2013,3,19]]},"references-count":40,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2013,3,19]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0059928","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3,19]]}}}