{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T16:15:57Z","timestamp":1770740157451,"version":"3.49.0"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2013,10,7]],"date-time":"2013-10-07T00:00:00Z","timestamp":1381104000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0076913","type":"journal-article","created":{"date-parts":[[2013,10,7]],"date-time":"2013-10-07T21:06:31Z","timestamp":1381179991000},"page":"e76913","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":21,"title":["An Efficient Strategy for Small-Scale Screening and Production of Archaeal Membrane Transport Proteins in Escherichia coli"],"prefix":"10.1371","volume":"8","author":[{"given":"Pikyee","family":"Ma","sequence":"first","affiliation":[]},{"given":"Filipa","family":"Varela","sequence":"additional","affiliation":[]},{"given":"Malgorzata","family":"Magoch","sequence":"additional","affiliation":[]},{"given":"Ana Rita","family":"Silva","sequence":"additional","affiliation":[]},{"given":"Ana L\u00facia","family":"Ros\u00e1rio","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9","family":"Brito","sequence":"additional","affiliation":[]},{"given":"T\u00e2nia Filipa","family":"Oliveira","sequence":"additional","affiliation":[]},{"given":"Przemyslaw","family":"Nogly","sequence":"additional","affiliation":[]},{"given":"Miguel","family":"Pessanha","sequence":"additional","affiliation":[]},{"given":"Meike","family":"Stelter","sequence":"additional","affiliation":[]},{"given":"Arnulf","family":"Kletzin","sequence":"additional","affiliation":[]},{"given":"Peter J. F.","family":"Henderson","sequence":"additional","affiliation":[]},{"given":"Margarida","family":"Archer","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2013,10,7]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1002\/pro.5560070420","article-title":"Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms","volume":"7","author":"E Wallin","year":"1998","journal-title":"Protein Sci"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1038\/nrd892","article-title":"The druggable genome","volume":"1","author":"AL Hopkins","year":"2002","journal-title":"Nat Rev Drug Discov"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1038\/nrd2199","article-title":"How many drug targets are there?","volume":"5","author":"JP Overington","year":"2006","journal-title":"Nat Rev Drug Discov"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.sbi.2011.07.001","article-title":"High throughput platforms for structural genomics of integral membrane proteins","volume":"21","author":"F Mancia","year":"2011","journal-title":"Curr Opin Struct Biol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nbt.1833","article-title":"Overcoming barriers to membrane protein structure determination","volume":"29","author":"RM Bill","year":"2011","journal-title":"Nat Biotechnol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1016\/j.biocel.2012.12.018","article-title":"Breaking the barriers in membrane protein crystallography","volume":"45","author":"HJ Kang","year":"2013","journal-title":"Int J Biochem Cell Biol"},{"key":"ref7","first-page":"15","article-title":"Membrane Transporters: Structure, Function and Targets for Drug Design","year":"2009"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"D274","DOI":"10.1093\/nar\/gkn862","article-title":"The Transporter Classification Database: recent advances","volume":"37","author":"MH Jr Saier","year":"2009","journal-title":"Nucleic Acids Res"},{"key":"ref9","article-title":"The Nucleobase-Cation-Symport-1 Family of Membrane Transport Proteins. Handbook of Metalloproteins. John Wiley & Sons, Ltd","author":"S Weyand","year":"2010"},{"key":"ref10","article-title":"The Amplified Expression, Identification, Purification, Assay and Properties of Hexahistidine-Tagged Bacterial Membrane Transport Proteins","year":"2000"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"SF Altschul","year":"1990","journal-title":"J Mol Biol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"3329","DOI":"10.1128\/JB.188.9.3329-3336.2006","article-title":"The hydantoin transport protein from Microbacterium liquefaciens","volume":"188","author":"S Suzuki","year":"2006","journal-title":"J Bacteriol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1006\/jmbi.2000.4315","article-title":"Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes","volume":"305","author":"A Krogh","year":"2001","journal-title":"J Mol Biol"},{"key":"ref14","article-title":"Jalview","author":"AM Waterhouse","year":"2009"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1006\/jmbi.1996.0399","article-title":"Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels","volume":"260","author":"B Miroux","year":"1996","journal-title":"J Mol Biol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/0003-2697(73)90217-0","article-title":"A rapid, sensitive, and specific method for the determination of protein in dilute solution","volume":"56","author":"W Schaffner","year":"1973","journal-title":"Anal Biochem"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4","volume":"227","author":"UK Laemmli","year":"1970","journal-title":"Nature"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/s00792-007-0087-9","article-title":"The impact of extremophiles on structural genomics (and vice versa)","volume":"12","author":"FE Jr Jenney","year":"2008","journal-title":"Extremophiles"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"4023","DOI":"10.1111\/j.1742-4658.2007.05955.x","article-title":"Thermodynamic stability and folding of proteins from hyperthermophilic organisms","volume":"274","author":"KA Luke","year":"2007","journal-title":"FEBS J"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"8152","DOI":"10.1021\/bi025523t","article-title":"Elucidation of factors responsible for enhanced thermal stability of proteins: a structural genomics based study","volume":"41","author":"S Chakravarty","year":"2002","journal-title":"Biochemistry"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1126\/science.1164440","article-title":"Structure and molecular mechanism of a nucleobase-cation-symport-1 family transporter","volume":"322","author":"S Weyand","year":"2008","journal-title":"Science"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1126\/science.1186303","article-title":"Molecular basis of alternating access membrane transport by the sodium-hydantoin transporter Mhp1","volume":"328","author":"T Shimamura","year":"2010","journal-title":"Science"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"2690","DOI":"10.1073\/pnas.052018199","article-title":"Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA\/GFP fusion analysis","volume":"99","author":"D Drew","year":"2002","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1080\/09687860701243998","article-title":"Topology-informed strategies for the overexpression and purification of membrane proteins","volume":"24","author":"M Rahman","year":"2007","journal-title":"Mol Membr Biol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1126\/science.1109730","article-title":"Global topology analysis of the Escherichia coli inner membrane proteome","volume":"308","author":"DO Daley","year":"2005","journal-title":"Science"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"11142","DOI":"10.1073\/pnas.0604075103","article-title":"A global topology map of the Saccharomyces cerevisiae membrane proteome","volume":"103","author":"H Kim","year":"2006","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1002\/pro.365","article-title":"Bridging the gap: a GFP-based strategy for overexpression and purification of membrane proteins with intra and extracellular C-termini","volume":"19","author":"JM Hsieh","year":"2010","journal-title":"Protein Sci"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1074\/mcp.M600431-MCP200","article-title":"Consequences of membrane protein overexpression in Escherichia coli","volume":"6","author":"S Wagner","year":"2007","journal-title":"Mol Cell Proteomics"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1111\/j.1751-7915.2009.00148.x","article-title":"Revolutionizing membrane protein overexpression in bacteria","volume":"3","author":"S Schlegel","year":"2010","journal-title":"J Microbiol Biotechnol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"14371","DOI":"10.1073\/pnas.0804090105","article-title":"Tuning Escherichia coli for membrane protein overexpression","volume":"105","author":"S Wagner","year":"2008","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/0076-6879(90)85008-C","article-title":"Use of T7 RNA polymerase to direct expression of cloned genes","volume":"185","author":"FW Studier","year":"1990","journal-title":"Methods Enzymol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1073\/pnas.80.1.21","article-title":"The tac promoter: a functional hybrid derived from the trp and lac promoters","volume":"80","author":"HA de Boer","year":"1983","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/0378-1119(87)90314-3","article-title":"Multicopy expression vectors carrying the lac repressor gene for regulated high-level expression of genes in Escherichia coli","volume":"51","author":"MJ Stark","year":"1987","journal-title":"Gene"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1093\/jac\/dkm036","article-title":"A genomic strategy for cloning, expressing and purifying efflux proteins of the major facilitator superfamily","volume":"59","author":"G Szakonyi","year":"2007","journal-title":"J Antimicrob Chemother"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1080\/09687680802359885","article-title":"Expression, purification and activities of the entire family of intact membrane sensor kinases from Enterococcus faecalis","volume":"25","author":"P Ma","year":"2008","journal-title":"Mol Membr Biol"},{"key":"ref36","first-page":"193","article-title":"Prokaryote multidrug efflux proteins of the major facilitator superfamily: amplified expression, purification and characterisation","volume":"3","author":"A Ward","year":"2001","journal-title":"J Mol Microbiol Biotechnol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s00253-006-0465-8","article-title":"Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems","volume":"72","author":"K Terpe","year":"2006","journal-title":"Appl Microbiol Biotechnol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1002\/pro.18","article-title":"A green fluorescent protein screen for identification of well-expressed membrane proteins from a cohort of extremophilic organisms","volume":"18","author":"J Hammon","year":"2009","journal-title":"Protein Sci"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"13936","DOI":"10.1073\/pnas.0704546104","article-title":"High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae","volume":"104","author":"S Newstead","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0005-2736(02)00719-8","article-title":"Comparison of seven different heterologous protein expression systems for the production of the serotonin transporter","volume":"1610","author":"CG Tate","year":"2003","journal-title":"Biochim Biophys Acta"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"e29191","DOI":"10.1371\/journal.pone.0029191","article-title":"Heterologous Expression of Membrane Proteins: Choosing the Appropriate Host","volume":"6","author":"F Bernaudat","year":"2011","journal-title":"PLOS ONE"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1038\/nmeth0406-303","article-title":"Optimization of membrane protein overexpression and purification using GFP fusions","volume":"3","author":"D Drew","year":"2006","journal-title":"Nat Methods"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"e56363","DOI":"10.1371\/journal.pone.0056363","article-title":"Using Haloarcula marismortui Bacteriorhodopsin as a Fusion Tag for Enhancing and Visible Expression of Integral Membrane Proteins in Escherichia coli","volume":"8","author":"M-F Hsu","year":"2013","journal-title":"PLOS ONE"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/j.str.2006.01.013","article-title":"Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins","volume":"14","author":"T Kawate","year":"2006","journal-title":"Structure"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.str.2010.12.001","article-title":"Benchmarking membrane protein detergent stability for improving throughput of high-resolution X-ray structures","volume":"19","author":"Y Sonoda","year":"2011","journal-title":"Structure"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.pep.2011.02.010","article-title":"An efficient strategy for high throughput screening of recombinant integral membrane protein expression and stability","volume":"78","author":"J Fan","year":"2011","journal-title":"Protein Expr Purif"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/S0014-5793(03)01148-7","article-title":"Collection and characterisation of bacterial membrane proteins","volume":"555","author":"M Saidijam","year":"2003","journal-title":"FEBS Lett"}],"updated-by":[{"DOI":"10.1371\/annotation\/ba41a7db-2c22-4ffc-b603-526534594a51","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2013,11,12]],"date-time":"2013-11-12T00:00:00Z","timestamp":1384214400000}}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0076913","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,18]],"date-time":"2024-05-18T13:27:43Z","timestamp":1716038863000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0076913"}},"subtitle":[],"editor":[{"given":"Hendrik W.","family":"van Veen","sequence":"first","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2013,10,7]]},"references-count":47,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2013,10,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0076913","relation":{"correction":[{"id-type":"doi","id":"10.1371\/annotation\/ba41a7db-2c22-4ffc-b603-526534594a51","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,7]]}}}