{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T23:50:48Z","timestamp":1775173848690,"version":"3.50.1"},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2014,1,31]],"date-time":"2014-01-31T00:00:00Z","timestamp":1391126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0087295","type":"journal-article","created":{"date-parts":[[2014,1,31]],"date-time":"2014-01-31T17:50:21Z","timestamp":1391190621000},"page":"e87295","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Biochemical, Stabilization and Crystallization Studies on a Molecular Chaperone (PaoD) Involved in the Maturation of Molybdoenzymes"],"prefix":"10.1371","volume":"9","author":[{"given":"Ana Rita","family":"Otrelo-Cardoso","sequence":"first","affiliation":[]},{"given":"Viola","family":"Schwuchow","sequence":"additional","affiliation":[]},{"given":"David","family":"Rodrigues","sequence":"additional","affiliation":[]},{"given":"Eurico J.","family":"Cabrita","sequence":"additional","affiliation":[]},{"given":"Silke","family":"Leimk\u00fchler","sequence":"additional","affiliation":[]},{"given":"Maria Jo\u00e3o","family":"Rom\u00e3o","sequence":"additional","affiliation":[]},{"given":"Teresa","family":"Santos-Silva","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2014,1,31]]},"reference":[{"key":"ref1","unstructured":"Coughlan MP (1980) Aldehyde oxidase, xanthine oxidase and xanthine dehydrogenase; hydroxylases containing molybdenum, iron-sulphur and flavin, in Molybdenum and Molybdenum-Containing Enzymes. Pergamon Press Oxford: 119\u2013185."},{"key":"ref2","first-page":"1783","article-title":"Characterization of the molybdenum cofactor of sulfite oxidase, xanthine oxidase and nitrate reductase. Identification of a pteridine as a structural component. J. Biol. Chem","volume":"255","author":"JL Johnson","year":"1980"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"6856","DOI":"10.1073\/pnas.79.22.6856","article-title":"Structural and metabolic relationship between the molybdenum cofactor and urothione. Proc. Natl. Acad. Sci","volume":"79","author":"JL Johnson","year":"1982","journal-title":"USA"},{"key":"ref4","first-page":"2757","article-title":"The Mononuclear Molybdenum Enzymes. Chem. Rev","volume":"96","author":"R Hille","year":"1996"},{"key":"ref5","first-page":"109","article-title":"Molybdenum and tungsten enzymes: the xanthine oxidase family. Curr. Opin. Chem. Biol","volume":"10","author":"CD Brondino","year":"2006"},{"key":"ref6","first-page":"233","article-title":"Molybdenum-cofactor - containing enzymes: Structure and Mechanism. Annu. Rev. Biochem","volume":"66","author":"C Kisker","year":"1997"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"Neumann M, Leimk\u00fchler S (2011) The role of system-specific molecular chaperones in the maturation of molybdoenzymes in bacteria, Biochem. Res. Int. 2011.","DOI":"10.1155\/2011\/850924"},{"key":"ref8","first-page":"2745","article-title":"Role of XDHC in Molybdenum cofactor insertion into xanthine dehydrogenase of Rhodobacter capsulatus. J. Bacteriol","volume":"181","author":"S Leimk\u00fchler","year":"1999"},{"key":"ref9","first-page":"15701","article-title":"Rhodobacter capsulatus XdhC is involved in molybdenum cofactor binding and insertion into xanthine dehydrogenase. J. Biol. Chem","volume":"281","author":"M Neumann","year":"2006"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"9586","DOI":"10.1021\/bi700630p","article-title":"Identification of a Rhodobacter capsulatus L-cysteine desulfurase that sulfurates the molybdenum cofactor when bound to XdhC and before its insertion into xanthine dehydrogenase","volume":"46","author":"M Neumann","year":"2007","journal-title":"Biochemistry"},{"key":"ref11","first-page":"28493","article-title":"Transfer of the molybdenum cofactor synthesized by Rhodobacter capsulatus MoeA to XdhC and MobA. J. Biol. Chem","volume":"282","author":"M Neumann","year":"2007"},{"key":"ref12","first-page":"2762","article-title":"A periplasmic aldehyde oxidoreductase represents the first molybdopterin cytosine dinucleotide cofactor containing molybdo-flavoenzyme from Escherichia coli. FEBS J","volume":"276","author":"M Neumann","year":"2009"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"Iobbi-nivol C, Leimk\u00fchler S (2012) Molybdenum enzymes, their maturation and molybdenum cofactor biosynthesis in Escherichia coli. Biochim. Biophys. Acta.","DOI":"10.1016\/j.bbabio.2012.11.007"},{"key":"ref14","first-page":"21891","article-title":"MocA is a specific cytidylyltransferase involved in molybdopterin cytosine dinucleotide biosynthesis in Escherichia coli. J. Biol. Chem","volume":"284","author":"M Neumann","year":"2009"},{"key":"ref15","first-page":"1400","article-title":"Molybdopterin dinucleotide biosynthesis in Escherichia coli: identification of amino acid residues of molybdopterin dinucleotide transferases that determine specificity for binding of guanine or cytosine nucleotides. J. Biol. Chem","volume":"286","author":"M Neumann","year":"2011"},{"key":"ref16","first-page":"864","article-title":"NMR Spectroscopy Techniques for Screening and Identifying Ligand Binding to Protein Receptors. Angew. Chem. Int","volume":"42","author":"B Meyer","year":"2003"},{"key":"ref17","first-page":"1784","article-title":"Characterization of Ligand Binding by Saturation Transfer Difference NMR Spectroscopy. Angew. Chem. Int","volume":"38","author":"M Mayer","year":"1999"},{"key":"ref18","first-page":"990","article-title":"Saturation-Transfer Difference (STD) NMR: A Simple and Fast Method for Ligand Screening and Characterization of Protein Binding. J. Chem. Educ","volume":"88","author":"A Viegas","year":"2011"},{"key":"ref19","first-page":"281","article-title":"Optimization of expression of human sulfite oxidase and its molybdenum domain. Arch. Biochem. Biophys","volume":"383","author":"CA Temple","year":"2000"},{"key":"ref20","first-page":"875","article-title":"Involvement of the narJ and mob gene products in distinct steps in the biosynthesis of the molybdoenzyme nitrate reductase in Escherichia coli. Mol","volume":"20","author":"T Palmer","year":"1996","journal-title":"Microbiol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"14080","DOI":"10.1021\/bi0513406","article-title":"Exploring the mechanism of binding of UDP-galactopyranose to UDP-galactopyranose mutase by STD-NMR spectroscopy and molecular modeling","volume":"44","author":"Y Yuan","year":"2005","journal-title":"Biochemistry"},{"key":"ref22","first-page":"409","article-title":"Sparse matrix sampling: a screening method for crystallization of proteins. J. Appl. Cryst","volume":"24","author":"J Jancarik","year":"1991"},{"key":"ref23","unstructured":"Leslie AGW (1992) Recent changes to the MOSFLM package for processing film and image plate data. Jnt CCP4\/ESF\u2013EACBM Newsl. Protein Crystallogr. 26."},{"key":"ref24","first-page":"72","article-title":"Scaling and assessment of data quality. Acta Cryst","volume":"D62","author":"PR Evans","year":"2006"},{"key":"ref25","first-page":"916","article-title":"Evaluation of single-crystal X-ray diffraction data from a position-sensitive detector. J. Appl. Cryst","volume":"21","author":"W Kabsch","year":"1988"},{"key":"ref26","first-page":"785","article-title":"Activity and thermal stability of lysozyme in alkylammonium formate ionic liquids\u2013influence of cation modification. Green Chem","volume":"11","author":"JP Mann","year":"2009"},{"key":"ref27","first-page":"707","article-title":"Protein unfolding, and the \u2018tuning in\u2019 of reversible intermediate states, in protic ionic liquid media. J. Mol. Biol","volume":"378","author":"N Byrne","year":"2008"},{"key":"ref28","first-page":"1648","article-title":"Physical chemistry of ionic liquids, Phys. Chem. Chem. Phys","volume":"12","author":"D Constatinescu","year":"2010"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1016\/j.jcrysgro.2009.11.060","article-title":"The use of ionic liquids as crystallization additives allowed to overcome nanodrop scaling up problems: A success case for producing diffraction-quality crystals of a nitrate reductase. J. Cryst","volume":"312","author":"C Coelho","year":"2010","journal-title":"Growth"},{"key":"ref30","first-page":"4912","article-title":"Hofmeister effects of ionic liquids in protein crystallization: Direct and water-mediated interactions. CrystEngComm","volume":"14","author":"M Kowacz","year":"2012"},{"key":"ref31","first-page":"380","article-title":"Saturation transfer difference nuclear magnetic resonance study on the specific binding of ligand to protein. Anal. Biochem","volume":"385","author":"Z Ji","year":"2009"},{"key":"ref32","first-page":"19632","article-title":"Protein destabilization in ionic liquids: the role of preferential interactions in denaturation. Phys. Chem. Chem. Phys","volume":"15","author":"AM Figueiredo","year":"2013"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0087295","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T18:34:30Z","timestamp":1588962870000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0087295"}},"subtitle":[],"editor":[{"given":"Annalisa","family":"Pastore","sequence":"first","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2014,1,31]]},"references-count":32,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2014,1,31]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0087295","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,31]]}}}