{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T11:41:25Z","timestamp":1767181285496,"version":"build-2238731810"},"reference-count":63,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2014,10,22]],"date-time":"2014-10-22T00:00:00Z","timestamp":1413936000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0110324","type":"journal-article","created":{"date-parts":[[2014,10,22]],"date-time":"2014-10-22T13:54:29Z","timestamp":1413986069000},"page":"e110324","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":40,"title":["Neurotrophic Requirements of Human Motor Neurons Defined Using Amplified and Purified Stem Cell-Derived Cultures"],"prefix":"10.1371","volume":"9","author":[{"given":"Nuno Jorge","family":"Lamas","sequence":"first","affiliation":[]},{"given":"Bethany","family":"Johnson-Kerner","sequence":"additional","affiliation":[]},{"given":"Laurent","family":"Roybon","sequence":"additional","affiliation":[]},{"given":"Yoon A.","family":"Kim","sequence":"additional","affiliation":[]},{"given":"Alejandro","family":"Garcia-Diaz","sequence":"additional","affiliation":[]},{"given":"Hynek","family":"Wichterle","sequence":"additional","affiliation":[]},{"given":"Christopher E.","family":"Henderson","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2014,10,22]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1016\/j.neuron.2011.05.003","article-title":"Constructing and deconstructing stem cell models of neurological disease","volume":"70","author":"SS Han","year":"2011","journal-title":"Neuron"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/S0092-8674(02)00835-8","article-title":"Directed differentiation of embryonic stem cells into motor neurons","volume":"110","author":"H Wichterle","year":"2002","journal-title":"Cell"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1038\/nbt1063","article-title":"Specification of motoneurons from human embryonic stem cells","volume":"23","author":"XJ Li","year":"2005","journal-title":"Nat Biotechnol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1126\/science.1158799","article-title":"Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons","volume":"321","author":"JT Dimos","year":"2008","journal-title":"Science"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1038\/nature07677","article-title":"Induced pluripotent stem cells from a spinal muscular atrophy patient","volume":"457","author":"AD Ebert","year":"2009","journal-title":"Nature"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/nbt.1783","article-title":"A functionally characterized test set of human induced pluripotent stem cells","volume":"29","author":"GL Boulting","year":"2011","journal-title":"Nat Biotechnol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"4335","DOI":"10.1073\/pnas.0910012107","article-title":"Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potency","volume":"107","author":"BY Hu","year":"2010","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1038\/nbt.1529","article-title":"Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling","volume":"27","author":"SM Chambers","year":"2009","journal-title":"Nat Biotechnol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/S0959-4388(96)80010-9","article-title":"Role of neurotrophic factors in neuronal development","volume":"6","author":"CE Henderson","year":"1996","journal-title":"Curr Opin Neurobiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"S55","DOI":"10.1080\/146608201300079454","article-title":"Motoneuron cell death and neurotrophic factors: basic models for development of new therapeutic strategies in ALS","volume":"2","author":"M Beck","year":"2001","journal-title":"Amyotroph Lateral Scler Other Motor Neuron Disord"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/s004410000217","article-title":"Developmental motoneuron cell death and neurotrophic factors","volume":"301","author":"M Sendtner","year":"2000","journal-title":"Cell Tissue Res"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1146\/annurev.neuro.051508.135722","article-title":"Motor neuron diversity in development and disease","volume":"33","author":"KC Kanning","year":"2010","journal-title":"Annu Rev Neurosci"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"6007","DOI":"10.1073\/pnas.0508774103","article-title":"Chronic activation in presymptomatic amyotrophic lateral sclerosis (ALS) mice of a feedback loop involving Fas, Daxx, and FasL","volume":"103","author":"C Raoul","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/373341a0","article-title":"In vivo neurotrophic effects of GDNF on neonatal and adult facial motor neurons","volume":"373","author":"Q Yan","year":"1995","journal-title":"Nature"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"9771","DOI":"10.1073\/pnas.92.21.9771","article-title":"Rescue of adult mouse motoneurons from injury-induced cell death by glial cell line-derived neurotrophic factor","volume":"92","author":"L Li","year":"1995","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.brainresrev.2010.10.003","article-title":"Motor neuron trophic factors: therapeutic use in ALS?","volume":"67","author":"TW Gould","year":"2011","journal-title":"Brain Res Rev"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S0896-6273(00)80281-0","article-title":"Cardiotrophin-1, a cytokine present in embryonic muscle, supports long-term survival of spinal motoneurons","volume":"17","author":"D Pennica","year":"1996","journal-title":"Neuron"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1038\/358502a0","article-title":"Ciliary neurotrophic factor prevents degeneration of motor neurons in mouse mutant progressive motor neuronopathy","volume":"358","author":"M Sendtner","year":"1992","journal-title":"Nature"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1616","DOI":"10.1126\/science.2011743","article-title":"Control of embryonic motoneuron survival in vivo by ciliary neurotrophic factor","volume":"251","author":"RW Oppenheim","year":"1991","journal-title":"Science"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/0896-6273(93)90326-M","article-title":"Evidence that brain-derived neurotrophic factor is a trophic factor for motor neurons in vivo","volume":"10","author":"VE Koliatsos","year":"1993","journal-title":"Neuron"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1038\/360755a0","article-title":"Brain-derived neurotrophic factor rescues developing avian motoneurons from cell death","volume":"360","author":"RW Oppenheim","year":"1992","journal-title":"Nature"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.expneurol.2005.06.021","article-title":"Enhancer-specified GFP-based FACS purification of human spinal motor neurons from embryonic stem cells","volume":"196","author":"N Singh Roy","year":"2005","journal-title":"Exp Neurol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/j.stem.2008.09.017","article-title":"Human embryonic stem cell-derived motor neurons are sensitive to the toxic effect of glial cells carrying an ALS-causing mutation","volume":"3","author":"FP Di Giorgio","year":"2008","journal-title":"Cell Stem Cell"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/j.stem.2013.04.003","article-title":"A small molecule screen in stem-cell-derived motor neurons identifies a kinase inhibitor as a candidate therapeutic for ALS","volume":"12","author":"YM Yang","year":"2013","journal-title":"Cell Stem Cell"},{"key":"ref25","article-title":"Pathways Disrupted in Human ALS Motor Neurons Identified through Genetic Correction of Mutant SOD1","author":"E Kiskinis","year":"2014","journal-title":"Cell Stem Cell"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1016\/S0896-6273(01)00407-X","article-title":"Coordinate regulation of motor neuron subtype identity and pan-neuronal properties by the bHLH repressor Olig2","volume":"31","author":"BG Novitch","year":"2001","journal-title":"Neuron"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1016\/S0896-6273(01)00414-7","article-title":"The bHLH transcription factor Olig2 promotes oligodendrocyte differentiation in collaboration with Nkx2.2","volume":"31","author":"Q Zhou","year":"2001","journal-title":"Neuron"},{"key":"ref28","unstructured":"Altman J, Bayer SA (2001) Development of the human spinal cord: an interpretation based on experimental studies in animals. Oxford; New York: Oxford University Press. xi, 542 p. p."},{"key":"ref29","unstructured":"Bayer SA, Altman J (2002) Atlas of human central nervous system development. Boca Raton: CRC Press. v. p."},{"key":"ref30","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1523\/JNEUROSCI.0906-12.2013","article-title":"Accelerated high-yield generation of limb-innervating motor neurons from human stem cells","volume":"33","author":"MW Amoroso","year":"2013","journal-title":"J Neurosci"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1126\/science.7973664","article-title":"GDNF: a potent survival factor for motoneurons present in peripheral nerve and muscle","volume":"266","author":"CE Henderson","year":"1994","journal-title":"Science"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1242\/dev.118.4.1149","article-title":"Embryonic wing and leg motoneurons have intrinsically different survival properties","volume":"118","author":"C Mettling","year":"1993","journal-title":"Development"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.mcn.2003.10.011","article-title":"Schwann cells and astrocytes induce synapse formation by spinal motor neurons in culture","volume":"25","author":"EM Ullian","year":"2004","journal-title":"Mol Cell Neurosci"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1038\/nrn3379","article-title":"Neurotrophin regulation of neural circuit development and function","volume":"14","author":"H Park","year":"2013","journal-title":"Nat Rev Neurosci"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1177\/1073858408324787","article-title":"Neurotrophic modulation of motor neuron development","volume":"15","author":"TW Gould","year":"2009","journal-title":"Neuroscientist"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0070-2153(09)88006-X","article-title":"Hox networks and the origins of motor neuron diversity","volume":"88","author":"JS Dasen","year":"2009","journal-title":"Curr Top Dev Biol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.cell.2008.06.019","article-title":"Hox repertoires for motor neuron diversity and connectivity gated by a single accessory factor, FoxP1","volume":"134","author":"JS Dasen","year":"2008","journal-title":"Cell"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1038\/363266a0","article-title":"Neurotrophins promote motor neuron survival and are present in embryonic limb bud","volume":"363","author":"CE Henderson","year":"1993","journal-title":"Nature"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1124\/jpet.107.123000","article-title":"Identification and characterization of cholest-4-en-3-one, oxime (TRO19622), a novel drug candidate for amyotrophic lateral sclerosis","volume":"322","author":"T Bordet","year":"2007","journal-title":"J Pharmacol Exp Ther"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"7361","DOI":"10.1523\/JNEUROSCI.18-18-07361.1998","article-title":"Cyclic AMP elevation is sufficient to promote the survival of spinal motor neurons in vitro","volume":"18","author":"MG Hanson Jr","year":"1998","journal-title":"J Neurosci"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1016\/S0896-6273(00)80586-3","article-title":"Depolarization and cAMP elevation rapidly recruit TrkB to the plasma membrane of CNS neurons","volume":"21","author":"A Meyer-Franke","year":"1998","journal-title":"Neuron"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/0165-3806(91)90074-S","article-title":"Effects of ciliary neuronotrophic factor on rat spinal cord neurons in vitro: survival and expression of choline acetyltransferase and low-affinity nerve growth factor receptors","volume":"63","author":"E Magal","year":"1991","journal-title":"Brain Res Dev Brain Res"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.stem.2010.07.013","article-title":"Functional diversity of ESC-derived motor neuron subtypes revealed through intraspinal transplantation","volume":"7","author":"M Peljto","year":"2010","journal-title":"Cell Stem Cell"},{"key":"ref44","first-page":"1297","article-title":"Pharmacological inhibition of Rho-kinase (ROCK) signaling enhances cisplatin resistance in neuroblastoma cells","volume":"37","author":"CA Street","year":"2010","journal-title":"Int J Oncol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1016\/j.neuint.2012.08.003","article-title":"ROCK inhibition with Y27632 promotes the proliferation and cell cycle progression of cultured astrocyte from spinal cord","volume":"61","author":"Z Yu","year":"2012","journal-title":"Neurochem Int"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"3680","DOI":"10.1167\/iovs.08-2634","article-title":"Enhancement on primate corneal endothelial cell survival in vitro by a ROCK inhibitor","volume":"50","author":"N Okumura","year":"2009","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"3128","DOI":"10.1523\/JNEUROSCI.15-04-03128.1995","article-title":"Survival of newly postmitotic motoneurons is transiently independent of exogenous trophic support","volume":"15","author":"C Mettling","year":"1995","journal-title":"J Neurosci"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1002\/mrd.21021","article-title":"ROCK inhibition enhances the recovery and growth of cryopreserved human embryonic stem cells and human induced pluripotent stem cells","volume":"76","author":"DA Claassen","year":"2009","journal-title":"Mol Reprod Dev"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1007\/s12015-009-9107-8","article-title":"Effect of ROCK inhibitor Y-27632 on normal and variant human embryonic stem cells (hESCs) in vitro: its benefits in hESC expansion","volume":"6","author":"K Gauthaman","year":"2010","journal-title":"Stem Cell Rev"},{"key":"ref50","first-page":"861","article-title":"Pharmacological inhibition of Rho-kinase signaling with Y-27632 blocks melanoma tumor growth","volume":"23","author":"A Routhier","year":"2010","journal-title":"Oncol Rep"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/s12015-010-9118-5","article-title":"The ROCK inhibitor Y-27632 negatively affects the expansion\/survival of both fresh and cryopreserved cord blood-derived CD34+ hematopoietic progenitor cells: Y-27632 negatively affects the expansion\/survival of CD34+HSPCs","volume":"6","author":"C Bueno","year":"2010","journal-title":"Stem Cell Rev"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1016\/S0168-8278(00)80245-7","article-title":"A p160ROCK-specific inhibitor, Y-27632, attenuates rat hepatic stellate cell growth","volume":"32","author":"H Iwamoto","year":"2000","journal-title":"J Hepatol"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"2517","DOI":"10.1097\/01.ju.0000085024.47406.6c","article-title":"Y-27632, a Rho-kinase inhibitor, inhibits proliferation and adrenergic contraction of prostatic smooth muscle cells","volume":"170","author":"RW Rees","year":"2003","journal-title":"J Urol"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1002\/jnr.21511","article-title":"Purkinje cell survival in organotypic cultures: implication of Rho and its downstream effector ROCK","volume":"86","author":"S Julien","year":"2008","journal-title":"J Neurosci Res"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1093\/brain\/awm284","article-title":"ROCK inhibition and CNTF interact on intrinsic signalling pathways and differentially regulate survival and regeneration in retinal ganglion cells","volume":"131","author":"P Lingor","year":"2008","journal-title":"Brain"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.brainres.2009.11.087","article-title":"Rho kinase inhibition protects CA1 cells in organotypic hippocampal slices during in vitro ischemia","volume":"1316","author":"L Gisselsson","year":"2010","journal-title":"Brain Res"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1523\/JNEUROSCI.23-04-01416.2003","article-title":"Rho kinase inhibition enhances axonal regeneration in the injured CNS","volume":"23","author":"AE Fournier","year":"2003","journal-title":"J Neurosci"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"6570","DOI":"10.1523\/JNEUROSCI.22-15-06570.2002","article-title":"Rho signaling pathway targeted to promote spinal cord repair","volume":"22","author":"P Dergham","year":"2002","journal-title":"J Neurosci"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1111\/j.1471-4159.2007.04756.x","article-title":"Inhibition of Rho kinase (ROCK) increases neurite outgrowth on chondroitin sulphate proteoglycan in vitro and axonal regeneration in the adult optic nerve in vivo","volume":"103","author":"P Lingor","year":"2007","journal-title":"J Neurochem"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.stem.2011.01.013","article-title":"LRRK2 mutant iPSC-derived DA neurons demonstrate increased susceptibility to oxidative stress","volume":"8","author":"HN Nguyen","year":"2011","journal-title":"Cell Stem Cell"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"1468","DOI":"10.1093\/hmg\/ddq021","article-title":"Rho-kinase inactivation prolongs survival of an intermediate SMA mouse model","volume":"19","author":"M Bowerman","year":"2010","journal-title":"Hum Mol Genet"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1002\/glia.22601","article-title":"Rho kinase inhibition modulates microglia activation and improves survival in a model of amyotrophic lateral sclerosis","volume":"62","author":"L Tonges","year":"2014","journal-title":"Glia"},{"key":"ref63","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1016\/j.stem.2014.03.004","article-title":"Pathways disrupted in human ALS motor neurons identified through genetic correction of mutant SOD1","volume":"14","author":"E Kiskinis","year":"2014","journal-title":"Cell Stem Cell"}],"updated-by":[{"DOI":"10.1371\/journal.pone.0119195","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2015,3,18]],"date-time":"2015-03-18T00:00:00Z","timestamp":1426636800000}}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0110324","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T03:52:07Z","timestamp":1717473127000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0110324"}},"subtitle":[],"editor":[{"given":"Jan","family":"Pruszak","sequence":"first","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2014,10,22]]},"references-count":63,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2014,10,22]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0110324","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,10,22]]}}}