{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T03:38:19Z","timestamp":1762659499520},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2016,8,12]],"date-time":"2016-08-12T00:00:00Z","timestamp":1470960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT POCTI (FEDER) and COMPETE","award":["PEst-C\/SAU\/LA000172013-2014"],"award-info":[{"award-number":["PEst-C\/SAU\/LA000172013-2014"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico, CNPq","award":["245531\/2012-2"],"award-info":[{"award-number":["245531\/2012-2"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0160762","type":"journal-article","created":{"date-parts":[[2016,8,12]],"date-time":"2016-08-12T17:38:27Z","timestamp":1471023507000},"page":"e0160762","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":30,"title":["Molecular Characterization of Giardia lamblia: First Report of Assemblage B in Human Isolates from Rio de Janeiro (Brazil)"],"prefix":"10.1371","volume":"11","author":[{"given":"Clarissa Perez","family":"Faria","sequence":"first","affiliation":[]},{"given":"Graziela Maria","family":"Zanini","sequence":"additional","affiliation":[]},{"given":"Gisele Silva","family":"Dias","sequence":"additional","affiliation":[]},{"given":"Sidnei","family":"da Silva","sequence":"additional","affiliation":[]},{"given":"Maria do C\u00e9u","family":"Sousa","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2016,8,12]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1128\/CMR.00033-10","article-title":"Zoonotic potential and molecular epidemiology of <italic>Giardia<\/italic> species and giardiasis","volume":"24","author":"Y Feng","year":"2011","journal-title":"Clin Microbiol Rev"},{"issue":"5","key":"ref2","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.pt.2006.02.015","article-title":"<italic>Giardia<\/italic> and <italic>Cryptosporidium<\/italic> join the \u201cNeglected Diseases Initiative\u201d","volume":"22","author":"L Savioli","year":"2006","journal-title":"Trends Parasitol"},{"issue":"9","key":"ref3","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1093\/oxfordjournals.molbev.a026204","article-title":"Molecular systematics of the parasitic protozoan <italic>Giardia intestinalis<\/italic>","volume":"16","author":"PT Monis","year":"1999","journal-title":"Mol Biol Evol"},{"issue":"9","key":"ref4","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/j.ijpara.2010.02.015","article-title":"The identification of a new <italic>Giardia duodenalis<\/italic> assemblage in marine vertebrates and a preliminary analysis of <italic>G<\/italic>. <italic>duodenalis<\/italic> population biology in marine systems","volume":"40","author":"E Lasek-Nesselquist","year":"2010","journal-title":"Int J Parasitol"},{"issue":"12","key":"ref5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pntd.0000558","article-title":"Identification of zoonotic genotypes of <italic>Giardia duodenalis<\/italic>","volume":"3","author":"H Sprong","year":"2009","journal-title":"PLoS Negl Trop Dis"},{"issue":"12\u201313","key":"ref6","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1016\/j.ijpara.2013.06.001","article-title":"Zoonotic potential of <italic>Giardia<\/italic>","volume":"43","author":"U Ryan","year":"2013","journal-title":"Int J Parasitol"},{"issue":"2","key":"ref7","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.ijpara.2007.07.016","article-title":"Mixed <italic>Giardia duodenalis<\/italic> assemblage A and E infections in calves","volume":"38","author":"T Geurden","year":"2008","journal-title":"Int J Parasitol"},{"issue":"6","key":"ref8","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1128\/AEM.02305-09","article-title":"Genotyping of <italic>Giardia duodenalis<\/italic> cysts by new real-time PCR assays for detection of mixed infections in human samples","volume":"76","author":"A Almeida","year":"2010","journal-title":"Appl Environ Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1795","DOI":"10.1017\/S0031182007003071","article-title":"Comparative evaluation of <italic>Giardia duodenalis<\/italic> sequence data","volume":"134","author":"CM Wielinga","year":"2007","journal-title":"Parasitology"},{"issue":"8","key":"ref10","doi-asserted-by":"crossref","first-page":"e1000560","DOI":"10.1371\/journal.ppat.1000560","article-title":"Draft genome sequencing of <italic>Giardia intestinalis<\/italic> assemblage B isolate GS: Is human giardiasis caused by two different species?","volume":"5","author":"O Franz\u00e9n","year":"2009","journal-title":"PLoS Pathog"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1093\/gbe\/evt197","article-title":"Genome sequencing of <italic>Giardia lamblia<\/italic> genotypes A2 and B isolates (DH and GS) and comparative analysis with the genomes of genotypes A1 and E (WB and pig)","volume":"5","author":"RD Adam","year":"2013","journal-title":"Genome Biol Evol"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-015-0714-8","article-title":"Molecular characterization of intestinal protozoa in two poor communities in the State of S\u00e3o Paulo, Brazil","volume":"8","author":"\u00c9B David","year":"2015","journal-title":"Parasit Vectors"},{"issue":"1","key":"ref13","first-page":"61","article-title":"Parasites in South Bahia: Focus on Giardiasis and Ascariasis among Preschoolers of Itabuna","volume":"3","author":"APM Mariano","year":"2015","journal-title":"Int J Heal Sci"},{"issue":"3\u20134","key":"ref14","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.vetpar.2007.08.019","article-title":"Molecular identification of <italic>Giardia duodenalis<\/italic> isolates from humans, dogs, cats and cattle from the state of S\u00e3o Paulo, Brazil, by sequence analysis of fragments of glutamate dehydrogenase (gdh) coding gene","volume":"149","author":"SLP Souza","year":"2007","journal-title":"Vet Parasitol"},{"issue":"7","key":"ref15","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1016\/j.trstmh.2008.03.002","article-title":"<italic>Giardia duodenalis<\/italic> assemblage, clinical presentation and markers of intestinal inflammation in Brazilian children","volume":"102","author":"A Kohli","year":"2008","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"8","key":"ref16","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.trstmh.2012.05.011","article-title":"Epidemiological, parasitological and molecular aspects of <italic>Giardia duodenalis<\/italic> infection in children attending public daycare centers in southeastern Brazil","volume":"106","author":"CKS Santos","year":"2012","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"12","key":"ref17","doi-asserted-by":"crossref","first-page":"e115489","DOI":"10.1371\/journal.pone.0115489","article-title":"Genetic Diversity of <italic>Giardia duodenalis<\/italic>: Multilocus Genotyping Reveals Zoonotic Potential between Clinical and Environmental Sources in a Metropolitan Region of Brazil","volume":"9","author":"M Durigan","year":"2014","journal-title":"PLoS One"},{"issue":"3","key":"ref18","doi-asserted-by":"crossref","first-page":"e0118065","DOI":"10.1371\/journal.pone.0118065","article-title":"Identical Assemblage of <italic>Giardia duodenalis<\/italic> in Humans, Animals and Vegetables in an Urban Area in Southern Brazil Indicates a Relationship among them","volume":"10","author":"CM Colli","year":"2015","journal-title":"PLoS One"},{"key":"ref19","first-page":"1","article-title":"Genotyping of <italic>Giardia duodenalis<\/italic> isolates in asymptomatic children attending daycare centre: evidence of high risk for anthroponotic transmission","author":"AP Oliveira-Arbex","year":"2015","journal-title":"Epidemiol Infect"},{"issue":"5","key":"ref20","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1590\/S0036-46652006000500006","article-title":"Occurrence of enteroparasites in day care centers in Botucatu (S\u00e3o Paulo State, Brazil) with emphasis on <italic>Cryptosporidium<\/italic> sp., <italic>Giardia duodenalis<\/italic> and <italic>Enterobius vermicularis<\/italic>","volume":"48","author":"TB Carvalho","year":"2006","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"issue":"1","key":"ref21","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1186\/1471-2458-14-399","article-title":"Diarrhea incidence and intestinal infections among rotavirus vaccinated infants from a poor area in Brazil: a spatial analysis","volume":"14","author":"CB Santos","year":"2014","journal-title":"BMC Public Health"},{"issue":"1","key":"ref22","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1590\/S0036-46652015000100004","article-title":"Enteropathogens detected in a daycare center, Southeastern Brazil: bacteria, virus, and parasite research","volume":"57","author":"EDR Castro","year":"2015","journal-title":"Rev do Inst Med Trop Sa\u00e3o Paulo"},{"issue":"1","key":"ref23","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.actatropica.2007.02.010","article-title":"Genotyping of <italic>Giardia duodenalis<\/italic> from human and animal samples from Brazil using \u03b2-giardin gene: A phylogenetic analysis","volume":"102","author":"AC Volot\u00e3o","year":"2007","journal-title":"Acta Trop"},{"key":"ref24","unstructured":"IBGE. Censo demogr\u00e1fico 2010. Informa\u00e7\u00f5es estat\u00edsticas sobre Rio de Janeiro. 2010. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/cidades.ibge.gov.br\/xtras\/uf.php?lang=&codu\" xlink:type=\"simple\">http:\/\/cidades.ibge.gov.br\/xtras\/uf.php?lang=&amp;codu<\/ext-link>. Accessed 15 Sept 2015."},{"key":"ref25","article-title":"Sele\u00e7\u00e3o de m\u00e9todos e t\u00e9cnicas de laborat\u00f3rio para o diagn\u00f3stico de parasitoses humanas","author":"G de Carli","year":"2001"},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"169","DOI":"10.4269\/ajtmh.1938.s1-18.169","article-title":"Critical study of clinical laboratory technics for the diagnosis of protozoan cysts and helminth eggs in feces","volume":"18","author":"EC Faust","year":"1938","journal-title":"Am J Trop Med Hyg"},{"key":"ref27","first-page":"289","article-title":"Prevalence and genotyping of <italic>Giardia duodenalis<\/italic> from beef calves in Alberta, Canada","volume":"112","author":"AJ Appelbee","year":"2003","journal-title":"Prevalence and genotyping of"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1016\/S0020-7519(02)00068-1","article-title":"Sequence analysis of the \u03b2-giardin gene and development of a polymerase chain reaction-restriction fragment length polymorphism assay to genotype <italic>Giardia duodenalis<\/italic> cysts from human faecal samples","volume":"32","author":"SM Cacci\u00f2","year":"2002","journal-title":"Int J Parasitol"},{"issue":"2","key":"ref29","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.ijpara.2004.10.022","article-title":"Genetic heterogeneity at the \u03b2-giardin locus among human and animal isolates of <italic>Giardia duodenalis<\/italic> and identification of potentially zoonotic subgenotypes","volume":"35","author":"M Lalle","year":"2005","journal-title":"Int J Parasitol"},{"issue":"2","key":"ref30","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.meegid.2004.02.001","article-title":"Discrimination of all genotypes of <italic>Giardia duodenalis<\/italic> at the glutamate dehydrogenase locus using PCR-RFLP","volume":"4","author":"CM Read","year":"2004","journal-title":"Infect Genet Evol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.meegid.2015.03.015","article-title":"Molecular diagnosis and genotype analysis of <italic>Giardia duodenalis<\/italic> in asymptomatic children from a rural area in central Colombia","volume":"32","author":"JD Ram\u00edrez","year":"2015","journal-title":"Infect Genet Evol"},{"issue":"3","key":"ref32","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1590\/0074-0276140507","article-title":"Intestinal parasites and genotyping of <italic>Giardia duodenalis<\/italic> in children: first report of genotype B in isolates from human clinical samples in Mexico","volume":"109","author":"JC Torres-Romero","year":"2014","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.meegid.2014.01.007","article-title":"Correlation of <italic>Giardia duodenalis<\/italic> assemblages with clinical and epidemiological data in Cuban children","volume":"23","author":"LJ Puebla","year":"2014","journal-title":"Infect Genet Evol"},{"issue":"10","key":"ref34","doi-asserted-by":"crossref","first-page":"3133","DOI":"10.1128\/JCM.00715-15","article-title":"Case-control study of risk factors for sporadic giardiasis and parasite assemblages in North West England","volume":"53","author":"C Minetti","year":"2015","journal-title":"J Clin Microbiol"},{"issue":"9","key":"ref35","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1016\/j.ijpara.2011.05.002","article-title":"Host parasite interactions and pathophysiology in <italic>Giardia<\/italic> infections","volume":"41","author":"JA Cotton","year":"2011","journal-title":"Int J Parasitol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1016\/j.ijpara.2008.04.008","article-title":"Multilocus genotyping of <italic>Giardia duodenalis<\/italic> reveals striking differences between assemblages A and B","volume":"38","author":"SM Cacci\u00f2","year":"2008","journal-title":"Int J Parasitol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.meegid.2013.04.013","article-title":"Multilocus genotyping of <italic>Giardia duodenalis<\/italic> in Malaysia","volume":"17","author":"CS Huey","year":"2013","journal-title":"Infect Genet Evol"},{"key":"ref38","first-page":"S1567","article-title":"Molecular epidemiology of <italic>Giardia<\/italic> and <italic>Cryptosporidium<\/italic> infections","volume":"11","author":"RCA Thompson","year":"2015","journal-title":"Infect Genet Evol"},{"issue":"3","key":"ref39","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.mimet.2009.06.018","article-title":"Preservation of <italic>Giardia<\/italic> cysts in stool samples for subsequent PCR analysis","volume":"78","author":"H Wilke","year":"2009","journal-title":"J Microbiol Methods"},{"issue":"8","key":"ref40","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1590\/S0074-02762012000800001","article-title":"Stool sample storage conditions for the preservation of <italic>Giardia intestinalis<\/italic> DNA","volume":"107","author":"S Kuk","year":"2012","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"10","key":"ref41","doi-asserted-by":"crossref","first-page":"3449","DOI":"10.1007\/s00436-013-3524-y","article-title":"Molecular typing of <italic>Giardia duodenalis<\/italic> isolates from German travellers","volume":"112","author":"A Broglia","year":"2013","journal-title":"Parasitol Res"},{"issue":"6","key":"ref42","doi-asserted-by":"crossref","first-page":"2165","DOI":"10.1007\/s00436-015-4405-3","article-title":"Multilocus sequence typing of canine <italic>Giardia duodenalis<\/italic> from South Eastern European countries","volume":"114","author":"MF Sommer","year":"2015","journal-title":"Parasitol Res"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.mimet.2015.01.016","article-title":"Comparison of four rapid diagnostic tests, ELISA, microscopy and PCR for the detection of <italic>Giardia lamblia<\/italic>, <italic>Cryptosporidium<\/italic> spp. and <italic>Entamoeba histolytica<\/italic> in feces","volume":"110","author":"DV den Bossche","year":"2015","journal-title":"J Microbiol Methods"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0160762","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T16:02:27Z","timestamp":1589040147000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0160762"}},"subtitle":[],"editor":[{"given":"Michael E.","family":"Grigg","sequence":"first","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2016,8,12]]},"references-count":43,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2016,8,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0160762","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,12]]}}}