{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T20:05:43Z","timestamp":1762459543226,"version":"3.37.3"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2018,1,9]],"date-time":"2018-01-09T00:00:00Z","timestamp":1515456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["ERA-IB-2\/0007\/2013"],"award-info":[{"award-number":["ERA-IB-2\/0007\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/52302\/2013"],"award-info":[{"award-number":["SFRH\/BD\/52302\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["ERA-NET ENMed\/0001\/2013"],"award-info":[{"award-number":["ERA-NET ENMed\/0001\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["LISBOA-01-0145-FEDER-007344"],"award-info":[{"award-number":["LISBOA-01-0145-FEDER-007344"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0190974","type":"journal-article","created":{"date-parts":[[2018,1,9]],"date-time":"2018-01-09T14:24:24Z","timestamp":1515507864000},"page":"e0190974","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["A detection and quantification label-free tool to speed up downstream processing of model mucins"],"prefix":"10.1371","volume":"13","author":[{"given":"Sofia B.","family":"Carvalho","sequence":"first","affiliation":[]},{"given":"Ana Sofia","family":"Moreira","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Gomes","sequence":"additional","affiliation":[]},{"given":"Manuel J. T.","family":"Carrondo","sequence":"additional","affiliation":[]},{"given":"David J.","family":"Thornton","sequence":"additional","affiliation":[]},{"given":"Paula M.","family":"Alves","sequence":"additional","affiliation":[]},{"given":"Julia","family":"Costa","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0995-6637","authenticated-orcid":true,"given":"Cristina","family":"Peixoto","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2018,1,9]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1097\/JTO.0000000000000404","article-title":"Mucins in Lung Cancer","volume":"10","author":"I Lakshmanan","year":"2015","journal-title":"J Thorac Oncol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/978-1-61779-513-8_2","article-title":"Gel-forming and cell-associated mucins: preparation for structural and functional studies","author":"JR Davies","year":"2012","journal-title":"Mucins Methods Protoc"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1053\/j.gastro.2016.03.039","article-title":"Defective Intestinal Mucin-type O-glycosylation Causes Spontaneous Colitis-associated Cancer in Mice","volume":"151","author":"K Bergstrom","year":"2016","journal-title":"Gastroenterology"},{"issue":"10","key":"ref4","doi-asserted-by":"crossref","first-page":"4421","DOI":"10.1039\/c3cs35470a","article-title":"The development of synthetic antitumour vaccines from mucin glycopeptide antigens","volume":"42","author":"N Gaidzik","year":"2013","journal-title":"Chem Soc Rev"},{"issue":"50","key":"ref5","doi-asserted-by":"crossref","first-page":"44932","DOI":"10.1039\/C6RA07424C","article-title":"An optimized purification process for porcine gastric mucin with preservation of its native functional properties","volume":"6","author":"VJ Sch\u00f6mig","year":"2016","journal-title":"RSC Adv"},{"key":"ref6","unstructured":"Glycoprotein Methods and Protocols The Mucins [Internet]. Corfield,. Vol. 125, Methods in Molecular Biology. Springer Science &amp; Business Media; 2000. 491 p. <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/download.springer.com\/static\/pdf\/300\/bok%3A978-1-59259-048-3.pdf?auth66=1408227233_74925630b3914ec38b7cc0f25cdf347d&ext=.pdf\" xlink:type=\"simple\">http:\/\/download.springer.com\/static\/pdf\/300\/bok%3A978-1-59259-048-3.pdf?auth66=1408227233_74925630b3914ec38b7cc0f25cdf347d&amp;ext=.pdf<\/ext-link>"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1513\/pats.2306016","article-title":"From Mucins to Mucus: Toward a More Coherent Understanding of This Essential Barrier","volume":"1","author":"DJ Thornton","year":"2004","journal-title":"Proc Am Thorac Soc"},{"issue":"Pt 3","key":"ref8","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1042\/bj3610537","article-title":"Heterogeneity of airways mucus: variations in the amounts and glycoforms of the major oligomeric mucins MUC5AC and MUC5B","volume":"361","author":"S Kirkham","year":"2002","journal-title":"Biochem J"},{"key":"ref9","article-title":"Contribution of mucus concentration and secreted mucins Muc5ac and Muc5b to the pathogenesis of muco-obstructive lung disease","author":"A Livraghi-Butrico","year":"2016","journal-title":"Mucosal Immunol"},{"issue":"6","key":"ref10","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1016\/j.biotechadv.2011.07.004","article-title":"Rational design and optimization of downstream processes of virus particles for biopharmaceutical applications: Current advances","volume":"29","author":"T Vicente","year":"2011","journal-title":"Biotechnol Adv"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/978-1-61779-513-8_3","article-title":"Detecting, Visualising, and Quantifying Mucins","author":"CA Harrop","year":"2012","journal-title":"Mucins Methods Protoc"},{"key":"ref12","first-page":"1","article-title":"Universal label-free in-process quantification of influenza virus-like particles","author":"SB Carvalho","year":"2017","journal-title":"Biotechnol J"},{"issue":"7510","key":"ref13","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1038\/nature13443","article-title":"Receptor binding by H10 influenza viruses","volume":"511","author":"SG Vachieri","year":"2014","journal-title":"Nature"},{"issue":"1\u20132","key":"ref14","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.virol.2013.08.010","article-title":"Changes in the hemagglutinin of H5N1 viruses during human infection\u2014In fl uence on receptor binding","volume":"447","author":"M Crusat","year":"2013","journal-title":"Virology"},{"issue":"7483","key":"ref15","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1038\/nature12807","article-title":"Muc5b is required for airway defence","volume":"505","author":"MG Roy","year":"2014","journal-title":"Nature"},{"issue":"15","key":"ref16","doi-asserted-by":"crossref","first-page":"4524","DOI":"10.1021\/acs.langmuir.5b00548","article-title":"Molecular structure and equilibrium forces of bovine submaxillary mucin adsorbed at a solid-liquid interface","volume":"31","author":"B Zappone","year":"2015","journal-title":"Langmuir"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/glycob\/12.1.1","article-title":"The salivary mucin MG1 (MUC5B) carries a repertoire of unique oligosaccharides that is large and diverse","volume":"12","author":"K a Thomsson","year":"2002","journal-title":"Glycobiology"},{"key":"ref18","article-title":"Identification of N-Linked Glycoproteins in Human Saliva by Glycoprotein Capture and Mass Spectrometry","author":"P Ramachandran","year":"2006","journal-title":"Identification of N-Linked Glycoproteins in Human Saliva by Glycoprotein Capture and Mass Spectrometry"},{"issue":"4","key":"ref19","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1093\/glycob\/cwh041","article-title":"C-mannosylation of MUC5AC and MUC5B Cys subdomains","volume":"14","author":"J Perez-Vilar","year":"2004","journal-title":"Glycobiology"},{"issue":"Pt 1","key":"ref20","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1042\/bj2790095","article-title":"Structural characterization of neutral oligosaccharides with blood-group A and H activity isolated from bovine submaxillary mucin","volume":"279","author":"a V Savage","year":"1991","journal-title":"Biochem J"},{"issue":"10","key":"ref21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0109265","article-title":"Purification and characterization of a mucin specific mycelial lectin from Aspergillus gorakhpurensis: Application for mitogenic and antimicrobial activity","volume":"9","author":"RS Singh","year":"2014","journal-title":"PLoS One"},{"key":"ref22","first-page":"1938","article-title":"Purification, Characterization, and Mitogenic Potential of a Mucin-Specific Mycelial Lectin from Aspergillus sparsus","author":"RS Singh","year":"2015","journal-title":"Purification, Characterization, and Mitogenic Potential of a Mucin-Specific Mycelial Lectin from Aspergillus sparsus"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.bios.2014.02.006","article-title":"Construction and validation of a Sambucus nigra biosensor for cancer-associated STn antigen","volume":"57","author":"MLS Silva","year":"2014","journal-title":"Biosens Bioelectron"},{"issue":"2","key":"ref24","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.jpba.2010.08.022","article-title":"Detection of low-affinity anti-drug antibodies and improved drug tolerance in immunogenicity testing by Octet<sup>\u00ae<\/sup> biolayer biolayer interferometry","volume":"54","author":"J Li","year":"2011","journal-title":"J Pharm Biomed Anal"},{"issue":"8","key":"ref25","doi-asserted-by":"crossref","first-page":"791","DOI":"10.2174\/138620709789104915","article-title":"Label-free detection of biomolecular interactions using BioLayer interferometry for kinetic characterization","volume":"12","author":"J Concepcion","year":"2009","journal-title":"Comb Chem High Throughput Screen"},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.pep.2008.04.009","article-title":"A rapid method for determining dynamic binding capacity of resins for the purification of proteins","volume":"60","author":"T Do","year":"2008","journal-title":"Protein Expr Purif"},{"key":"ref27","unstructured":"Tobias R. Biomolecular Binding Kinetics Assays on the Octet Platform. ForteBio Interact. 2013;Pall Life Sciences."},{"issue":"1","key":"ref28","doi-asserted-by":"crossref","first-page":"21","DOI":"10.4103\/2229-5186.79345","article-title":"Methods for the determination of limit of detection and limit of quantitation of the analytical methods","volume":"2","author":"A Shrivastava","year":"2011","journal-title":"Chronicles Young Sci"},{"issue":"2","key":"ref29","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1039\/C3AN01835K","article-title":"Predicting detection limits of enzyme-linked immunosorbent assay (ELISA) and bioanalytical techniques in general","volume":"139","author":"S Zhang","year":"2014","journal-title":"Analyst"},{"issue":"Suppl 1(August)","key":"ref30","first-page":"S49","article-title":"Limit of blank, limit of detection and limit of quantitation","volume":"29","author":"DA Armbruster","year":"2008","journal-title":"Clin Biochem Rev"},{"key":"ref31","first-page":"1741","article-title":"Extracellular Vesicles from Ovarian Carcinoma Cells Display Specific Glycosignatures","author":"J Gomes","year":"2015","journal-title":"Extracellular Vesicles from Ovarian Carcinoma Cells Display Specific Glycosignatures"},{"article-title":"Essentials of Glycobiology","year":"2009","author":"RD Cummings","key":"ref32"},{"issue":"1","key":"ref33","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/BF00926038","article-title":"Structure, biosynthesis, and function of salivary mucins","volume":"137","author":"AM Wu","year":"1994","journal-title":"Mol Cell Biochem"},{"issue":"1","key":"ref34","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1093\/glycob\/cwq129","article-title":"Production and characterization of a monomeric form and a single-site form of Aleuria aurantia lectin","volume":"21","author":"J Olausson","year":"2011","journal-title":"Glycobiology"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1016\/j.colsurfb.2014.08.017","article-title":"Influence of impurities and contact scale on the lubricating properties of bovine submaxillary mucin (BSM) films on a hydrophobic surface","volume":"122","author":"N Nikogeorgos","year":"2014","journal-title":"Colloids Surfaces B Biointerfaces"},{"issue":"1","key":"ref36","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.jcis.2009.03.061","article-title":"Comparison of the adsorption kinetics and surface arrangement of \u201cas received\u201d and purified bovine submaxillary gland mucin (BSM) on hydrophilic surfaces","volume":"336","author":"M Lundin","year":"2009","journal-title":"J Colloid Interface Sci"},{"issue":"30","key":"ref37","doi-asserted-by":"crossref","first-page":"8303","DOI":"10.1021\/acs.langmuir.5b01281","article-title":"Proteolytic Degradation of Bovine Submaxillary Mucin (BSM) and Its Impact on Adsorption and Lubrication at a Hydrophobic Surface","volume":"31","author":"JB Madsen","year":"2015","journal-title":"Langmuir"},{"issue":"April","key":"ref38","first-page":"37","article-title":"A Simplified Chromatographic Approach To Purify Commercially Available Bovine Submaxillary Mucins (Bsm)","author":"JB Madsen","year":"2014","journal-title":"Prep Biochem Biotechnol"},{"issue":"3","key":"ref39","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1042\/bst0060607","article-title":"A colorimetric assay for glycoproteins based on the periodic acid\/Schiff stain","volume":"6","author":"M Mantle","year":"1978","journal-title":"Biochem Soc Trans"},{"issue":"5","key":"ref40","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1016\/j.exer.2007.01.018","article-title":"Assay of mucins in human tear fluid","volume":"84","author":"S Spurr-Michaud","year":"2007","journal-title":"Exp Eye Res"},{"issue":"12","key":"ref41","doi-asserted-by":"crossref","first-page":"3522","DOI":"10.1039\/c3an36258b","article-title":"An improved lectin-based method for the detection of mucin-type O-glycans in biological samples","volume":"138","author":"Cheng-Siang Lee","year":"2013","journal-title":"Analyst"},{"key":"ref42","first-page":"181","article-title":"Glycoproteins In Carbohydrate analysis, a practical approach","author":"J Montreuil","year":"2003"},{"key":"ref43","article-title":"Site-Specific Fucosylation Analysis Identifying Glycoproteins Associated with Aggressive Prostate Cancer Cell Lines Using Tandem Affinity Enrichments of Intact Glycopeptides Followed by Mass Spectrometry","author":"J Zhou","year":"2017","journal-title":"Site-Specific Fucosylation Analysis Identifying Glycoproteins Associated with Aggressive Prostate Cancer Cell Lines Using Tandem Affinity Enrichments of Intact Glycopeptides Followed by Mass Spectrometry"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0190974","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T23:32:27Z","timestamp":1540251147000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0190974"}},"subtitle":[],"editor":[{"given":"Sabato","family":"D\u2019Auria","sequence":"first","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2018,1,9]]},"references-count":43,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2018,1,9]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0190974","relation":{},"ISSN":["1932-6203"],"issn-type":[{"type":"electronic","value":"1932-6203"}],"subject":[],"published":{"date-parts":[[2018,1,9]]}}}