{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T16:00:15Z","timestamp":1777996815156,"version":"3.51.4"},"reference-count":55,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2020,12,16]],"date-time":"2020-12-16T00:00:00Z","timestamp":1608076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BPD\/111509\/2015"],"award-info":[{"award-number":["SFRH\/BPD\/111509\/2015"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000733","name":"Universities Federation for Animal Welfare","doi-asserted-by":"publisher","award":["Grant 14-16\/17"],"award-info":[{"award-number":["Grant 14-16\/17"]}],"id":[{"id":"10.13039\/501100000733","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007374","name":"Associazione Consiglieri della Regione Autonoma Friuli Venezia Giulia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007374","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Dog training methods range broadly from those using mostly positive punishment and negative reinforcement (aversive-based) to those using primarily positive reinforcement (reward-based). Although aversive-based training has been strongly criticized for negatively affecting dog welfare, there is no comprehensive research focusing on companion dogs and mainstream techniques, and most studies rely on owner-reported assessment of training methods and dog behavior. The aim of the present study was to evaluate the effects of aversive- and reward-based training methods on companion dog welfare within and outside the training context. Ninety-two companion dogs were recruited from three reward-based schools (Group Reward, n = 42), and from four aversive-based schools, two using low proportions of aversive-based methods (Group Mixed, n = 22) and two using high proportions of aversive-based methods (Group Aversive, n = 28). For evaluating welfare during training, dogs were video recorded for three sessions and six saliva samples were collected, three at home (baseline levels) and three after training (post-training levels). Video recordings were used to examine the frequency of stress-related behaviors (e.g., lip lick, yawn) and the overall behavioral state of the dog (e.g., tense, relaxed), and saliva samples were analyzed for cortisol concentration. For evaluating welfare outside the training context, dogs participated in a cognitive bias task. Results showed that dogs from Group Aversive displayed more stress-related behaviors, were more frequently in tense and low behavioral states and panted more during training, and exhibited higher post-training increases in cortisol levels than dogs from Group Reward. Additionally, dogs from Group Aversive were more \u2018pessimistic\u2019 in the cognitive bias task than dogs from Group Reward. Dogs from Group Mixed displayed more stress-related behaviors, were more frequently in tense states and panted more during training than dogs from Group Reward. Finally, although Groups Mixed and Aversive did not differ in their performance in the cognitive bias task nor in cortisol levels, the former displayed more stress-related behaviors and was more frequently in tense and low behavioral states. These findings indicate that aversive-based training methods, especially if used in high proportions, compromise the welfare of companion dogs both within and outside the training context.<\/jats:p>","DOI":"10.1371\/journal.pone.0225023","type":"journal-article","created":{"date-parts":[[2020,12,16]],"date-time":"2020-12-16T14:39:15Z","timestamp":1608129555000},"page":"e0225023","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":60,"title":["Does training method matter? Evidence for the negative impact of aversive-based methods on companion dog welfare"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3357-7282","authenticated-orcid":true,"given":"Ana Catarina","family":"Vieira de Castro","sequence":"first","affiliation":[]},{"given":"Danielle","family":"Fuchs","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5439-969X","authenticated-orcid":true,"given":"Gabriela Munhoz","family":"Morello","sequence":"additional","affiliation":[]},{"given":"Stefania","family":"Pastur","sequence":"additional","affiliation":[]},{"given":"Liliana","family":"de Sousa","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4369-8723","authenticated-orcid":true,"given":"I. 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The Use of Electric Pulse Training Aids (EPTAs) in Companion Animals. Available from: http:\/\/eprints.lincoln.ac.uk\/14640\/1\/CAWC%20ecollar%20report.pdf (Accessed 12 August 2019)."},{"key":"pone.0225023.ref012","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.applanim.2007.11.010","article-title":"Training methods of military dog handlers and their effects on the team\u2019s performances","volume":"113","author":"A Haverbeke","year":"2008","journal-title":"Appl Anim Behav Sci"},{"key":"pone.0225023.ref013","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/S0168-1591(00)00203-3","article-title":"Behavioural changes and aversive conditioning in hunting dogs by the second-year confrontation with domestic sheep","volume":"72","author":"FO Christiansen","year":"2001","journal-title":"Appl Anim Behav Sci"},{"key":"pone.0225023.ref014","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.neubiorev.2020.01.025","article-title":"Animal affect and decision-making.","volume":"112","author":"M Mendl","journal-title":"Neurosci Biobehav Rev. 2020"},{"key":"pone.0225023.ref015","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1038\/427312a","article-title":"Animal behaviour: cognitive bias and affective state","volume":"427","author":"EJ Harding","year":"2004","journal-title":"Nature"},{"key":"pone.0225023.ref016","first-page":"81","volume-title":"How studying interaction between animal emotion, cognition, and personality can contribute to improve farm animal welfare.","author":"A Boissy","year":"2014","edition":"2"},{"key":"pone.0225023.ref017","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.applanim.2009.02.023","article-title":"Cognitive bias as an indicator of animal emotion and welfare: emerging evidence and underlying mechanisms","volume":"118","author":"M Mendl","year":"2009","journal-title":"Appl Anim Behav Sci"},{"key":"pone.0225023.ref018","doi-asserted-by":"crossref","first-page":"R839","DOI":"10.1016\/j.cub.2010.08.030","article-title":"Dogs showing separation-related behaviour exhibit a \u2018pessimistic\u2019 cognitive bias\u2019.","volume":"20","author":"M Mendl","year":"2010","journal-title":"Curr Biol"},{"issue":"4","key":"pone.0225023.ref019","doi-asserted-by":"crossref","first-page":"487","DOI":"10.3138\/jvme.35.4.487","article-title":"The benefits of human-companion animal interaction: a review","volume":"35","author":"SB Barker","year":"2008","journal-title":"J Vet Med Educ"},{"issue":"2","key":"pone.0225023.ref020","doi-asserted-by":"crossref","first-page":"98","DOI":"10.2752\/089279306785593757","article-title":"Benefits derived from companion animals, and the use of the term \u201cattachment\u201d.","volume":"19","author":"EK Crawford","year":"2006","journal-title":"Anthrozoos"},{"key":"pone.0225023.ref021","volume-title":"Science and Human Behavior.","author":"BF Skinner","year":"1953"},{"issue":"1","key":"pone.0225023.ref022","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.yhbeh.2008.09.010","article-title":"Methods of collection for salivary cortisol measurement in dogs.","volume":"55","author":"NA Dreschel","year":"2009","journal-title":"Horm Behav."},{"key":"pone.0225023.ref023","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/S0168-1591(97)00145-7","article-title":"Behavioural, saliva cortisol and heart rate responses to different types of stimuli in dogs","volume":"58","author":"B Beerda","year":"1998","journal-title":"Appl Anim Behav Sci"},{"key":"pone.0225023.ref024","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0034-5288(03)00080-8","article-title":"Sources of sampling variation in saliva cortisol in dogs","volume":"75","author":"A Kobelt","year":"2003","journal-title":"Res Vet Sci"},{"key":"pone.0225023.ref025","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0168-1591(96)01131-8","article-title":"Manifestations of chronic and acute stress in dogs","volume":"52","author":"B Beerda","year":"1997","journal-title":"Appl Anim Behav Sci"},{"key":"pone.0225023.ref026","first-page":"140","volume-title":"Fears and phobias in companion animals.","author":"VL Voith","year":"1996"},{"key":"pone.0225023.ref027","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.applanim.2003.10.004","article-title":"Training dogs with help of the shock collar: short and long term behavioural effects","volume":"85","author":"MBH Schilder","year":"2004","journal-title":"Appl Anim Behav Sci"},{"key":"pone.0225023.ref028","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.jveb.2009.09.006","article-title":"Attention seeking behavior in dogs\u2013what owners love and loathe!","volume":"5","author":"DS Mills","year":"2020","journal-title":"J Vet Behav: Clin Ap Res."},{"key":"pone.0225023.ref029","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.applanim.2006.11.002","article-title":"Clinical signs caused by the use of electric training collars on dogs in everyday life situations","volume":"105","author":"E Schalke","year":"2007","journal-title":"Appl Anim Behav Sci"},{"key":"pone.0225023.ref030","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.applanim.2006.06.018","article-title":"Evaluation of plasma cortisol levels and behavior in dogs wearing bark control collars","volume":"106","author":"JE Steiss","year":"2007","journal-title":"Appl Anim Behav Sci"},{"key":"pone.0225023.ref031","first-page":"530","article-title":"Comparison of learning effects and stress between 3 different training methods (electronic training collar, pinch collar and quitting signal) in Belgian Malinois Police Dogs.","volume":"163","author":"Y Salgirli","year":"2012","journal-title":"Rev M\u00e9d V\u00e9t."},{"key":"pone.0225023.ref032","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.applanim.2016.09.007","article-title":"The effects of housing conditions on judgement bias in Japanese quail","volume":"185","author":"M Horv\u00e1th","year":"2016","journal-title":"Appl Anim Behav Sci"},{"key":"pone.0225023.ref033","doi-asserted-by":"crossref","first-page":"20170779","DOI":"10.1098\/rsbl.2017.0779","article-title":"State-dependent judgement bias in Drosophila: evidence for evolutionarily primitive affective processes.","volume":"14","author":"A Deakin","year":"2018","journal-title":"Biol Lett"},{"key":"pone.0225023.ref034","doi-asserted-by":"crossref","first-page":"5404","DOI":"10.1038\/s41598-018-23545-6","article-title":"Environmental complexity buffers against stress-induced negative judgement bias in female chickens.","volume":"8","author":"J Zidar","year":"2018","journal-title":"Sci Rep"},{"key":"pone.0225023.ref035","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.neubiorev.2019.11.008","article-title":"Pharmacological manipulations of judgement bias: A systematic review and meta-analysis.","volume":"108","author":"V Neville","year":"2020","journal-title":"Neurosci Biobehav Rev."},{"issue":"11","key":"pone.0225023.ref036","doi-asserted-by":"crossref","first-page":"e0165840","DOI":"10.1371\/journal.pone.0165840","article-title":"Cognitive Bias in Ambiguity Judgements: Using Computational Models to Dissect the Effects of Mild Mood Manipulation in Humans.","volume":"11","author":"K Iigaya","year":"2016","journal-title":"PLoS ONE."},{"key":"pone.0225023.ref037","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.applanim.2011.03.007","article-title":"Training methods and owner-dog interactions: links with dog behaviour and learning ability","volume":"132","author":"NJ Rooney","year":"2011","journal-title":"Appl Anim Behav Sci"},{"key":"pone.0225023.ref038","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.beproc.2008.02.022","article-title":"Does training make you smarter? The effects of training on dogs\u2019 performance (Canis familiaris) in a problem solving task.","volume":"78","author":"S Marshall-Pescini","year":"2008","journal-title":"Behav Process"},{"key":"pone.0225023.ref039","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1037\/h0062474","article-title":"The formation of learning sets.","volume":"56","author":"HF Harlow","year":"1949","journal-title":"Psychol Rev"},{"issue":"1","key":"pone.0225023.ref040","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1901\/jeab.2012.98-105","article-title":"Relative efficacy of human social interaction and food as reinforcers for domestic dogs and hand-reared wolves.","volume":"98","author":"EN Feuerbacher","year":"2012","journal-title":"J Exp Anal Behav"},{"key":"pone.0225023.ref041","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.physbeh.2015.04.010","article-title":"Evidence for a synchronization of hormonal states between humans and dogs during competition","volume":"147","author":"AP Buttner","year":"2015","journal-title":"Physiol Behav"},{"issue":"1","key":"pone.0225023.ref042","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S0168-1591(98)00179-8","article-title":"Influence of male and female petters on plasma cortisol and behaviour: can human interaction reduce the stress of dogs in a public animal shelter?","volume":"61","author":"MB Hennessy","year":"1998","journal-title":"Appl Anim Behav Sci"},{"issue":"4","key":"pone.0225023.ref043","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.jveb.2014.03.002","article-title":"Dog and owner characteristics affecting the dog\u2013owner relationship.","volume":"9","author":"I Meyer","year":"2014","journal-title":"J Vet Behav"},{"issue":"2","key":"pone.0225023.ref044","doi-asserted-by":"crossref","first-page":"e0170707","DOI":"10.1371\/journal.pone.0170707","article-title":"Psychobiological Factors Affecting Cortisol Variability in Human-Dog Dyads.","volume":"12","author":"I Sch\u00f6berl","year":"2017","journal-title":"PLoS One."},{"issue":"4","key":"pone.0225023.ref045","doi-asserted-by":"crossref","first-page":"537","DOI":"10.3758\/s13420-018-0357-7","article-title":"Effect of age on discrimination learning, reversal learning, and cognitive bias in family dogs.","volume":"46","author":"P Piotti","year":"2018","journal-title":"Learn Behav."},{"issue":"1\u20133","key":"pone.0225023.ref046","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0960-0760(91)90168-5","article-title":"Age-related changes in the dog hypothalamic-pituitary-adrenocortical system: Neuroendocrine activity and corticosteroid receptors.","volume":"40","author":"JMHM Reul","year":"1991","journal-title":"J Steroid Biochem Mol Biol"},{"key":"pone.0225023.ref047","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.applanim.2011.03.006","article-title":"Trainability and boldness traits differ between dog breed clusters based on conventional breed categories and genetic relatedness","volume":"132","author":"B Turcs\u00e1n","year":"2011","journal-title":"Appl Anim Behav Sci"},{"key":"pone.0225023.ref048","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.applanim.2014.03.007","article-title":"Reliability and validity of a questionnaire survey in canine anxiety research","volume":"155","author":"K Tiira","year":"2014","journal-title":"Appl Anim Behav Sci"},{"key":"pone.0225023.ref049","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.applanim.2017.06.003","article-title":"Homing age influences the prevalence of aggressive and avoidance-related behaviour in adult dogs","volume":"195","author":"O Jokinen","year":"2017","journal-title":"Appl Anim Behav Sci"},{"issue":"1","key":"pone.0225023.ref050","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-020-60546-w","article-title":"Inadequate socialisation, inactivity, and urban living environment are associated with social fearfulness in pet dogs.","volume":"10","author":"J Puurunen","year":"2020","journal-title":"Sci Rep."},{"key":"pone.0225023.ref051","doi-asserted-by":"crossref","first-page":"508","DOI":"10.3389\/fvets.2020.00508","article-title":"Efficacy of Dog Training With and Without Remote Electronic Collars vs. a Focus on Positive Reinforcement.","volume":"7","author":"L China","year":"2020","journal-title":"Front Vet Sci."},{"key":"pone.0225023.ref052","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1186\/1746-6148-8-93","article-title":"The use of electronic collars for training domestic dogs: estimated prevalence, reasons and risk factors for use, and owner perceived success as compared to other training methods","volume":"8","author":"EJ Blackwell","year":"2012","journal-title":"BMC Vet Res"},{"key":"pone.0225023.ref053","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.jveb.2009.08.008","article-title":"Efficiency of working dogs undergoing a new Human Familiarization and Training Program.","volume":"5","author":"A Haverbeke","year":"2010","journal-title":"J Vet Behav"},{"key":"pone.0225023.ref054","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1007\/s10071-010-0326-9","article-title":"Reinforcement as a mediator of the perception of humans by horses (Equus caballus).","volume":"13","author":"C Sankey","year":"2010","journal-title":"Anim Cogn."},{"key":"pone.0225023.ref055","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.tvjl.2009.03.009","article-title":"A comparison of sympathetic and conventional training methods on responses to initial horse training.","volume":"181","author":"EK Visser","year":"2009","journal-title":"Vet J."}],"container-title":["PLOS 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