{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T16:31:14Z","timestamp":1772209874190,"version":"3.50.1"},"reference-count":60,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2020,12,21]],"date-time":"2020-12-21T00:00:00Z","timestamp":1608508800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    The bacterial strain PO100\/5 was isolated from a skin abscess taken from a pig (\n                    <jats:italic>Sus scrofa domesticus<\/jats:italic>\n                    ) in the Alentejo region of southern Portugal. It was identified as\n                    <jats:italic>Corynebacterium pseudotuberculosis<\/jats:italic>\n                    using biochemical tests, multiplex PCR and Pulsed Field Gel Electrophoresis. After genome sequencing and\n                    <jats:italic>rpoB<\/jats:italic>\n                    phylogeny, the strain was classified as\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>ulcerans<\/jats:italic>\n                    . To better understand the taxonomy of this strain and improve identification methods, we compared strain PO100\/5 to other publicly available genomes from\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>diphtheriae<\/jats:italic>\n                    group. Taxonomic analysis reclassified it and three others strains as the recently described\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>silvaticum<\/jats:italic>\n                    , which have been isolated from wild boar and roe deer in Germany and Austria. The results showed that PO100\/5 is the first sequenced genome of a\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>silvaticum<\/jats:italic>\n                    strain from livestock and a different geographical region, has the unique sequence type ST709, and could be could produce the\n                    <jats:italic>diphtheriae<\/jats:italic>\n                    toxin, along with strain 05\u201313. Genomic analysis of PO100\/5 showed four prophages, and eight conserved genomic islands in comparison to\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>ulcerans<\/jats:italic>\n                    . Pangenome analysis of 38\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>silvaticum<\/jats:italic>\n                    and 76\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>ulcerans<\/jats:italic>\n                    genomes suggested that C.\n                    <jats:italic>silvaticum<\/jats:italic>\n                    is a genetically homogeneous species, with 73.6% of its genes conserved and a pangenome near to be closed (\u03b1 &gt; 0.952). There are 172 genes that are unique to\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>silvaticum<\/jats:italic>\n                    in comparison to\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>ulcerans<\/jats:italic>\n                    . Most of these conserved genes are related to nutrient uptake and metabolism, prophages or immunity against them, and could be genetic markers for species identification. Strains PO100\/5 (livestock) and KL0182\n                    <jats:sup>T<\/jats:sup>\n                    (wild boar) were predicted to be potential human pathogens. This information may be useful for identification and surveillance of this pathogen.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0244210","type":"journal-article","created":{"date-parts":[[2020,12,21]],"date-time":"2020-12-21T13:43:04Z","timestamp":1608558184000},"page":"e0244210","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Taxonomic classification of strain PO100\/5 shows a broader geographic distribution and genetic markers of the recently described Corynebacterium silvaticum"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7017-6437","authenticated-orcid":true,"given":"Marcus Vinicius Can\u00e1rio","family":"Viana","sequence":"first","affiliation":[]},{"given":"Rodrigo","family":"Profeta","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2698-6889","authenticated-orcid":true,"given":"Alessandra Lima","family":"da Silva","sequence":"additional","affiliation":[]},{"given":"Raquel","family":"Hurtado","sequence":"additional","affiliation":[]},{"given":"Jana\u00edna Can\u00e1rio","family":"Cerqueira","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7068-7539","authenticated-orcid":true,"given":"Bruna Ferreira Sampaio","family":"Ribeiro","sequence":"additional","affiliation":[]},{"given":"Marcelle Oliveira","family":"Almeida","sequence":"additional","affiliation":[]},{"given":"Francielly","family":"Morais-Rodrigues","sequence":"additional","affiliation":[]},{"given":"Siomar de Castro","family":"Soares","sequence":"additional","affiliation":[]},{"given":"Manuela","family":"Oliveira","sequence":"additional","affiliation":[]},{"given":"Lu\u00eds","family":"Tavares","sequence":"additional","affiliation":[]},{"given":"Henrique","family":"Figueiredo","sequence":"additional","affiliation":[]},{"given":"Alice Rebecca","family":"Wattam","sequence":"additional","affiliation":[]},{"given":"Debmalya","family":"Barh","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3880-5886","authenticated-orcid":true,"given":"Preetam","family":"Ghosh","sequence":"additional","affiliation":[]},{"given":"Artur","family":"Silva","sequence":"additional","affiliation":[]},{"given":"Vasco","family":"Azevedo","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2020,12,21]]},"reference":[{"key":"pone.0244210.ref001","first-page":"1","volume-title":"Corynebacterium. 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