{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T12:28:57Z","timestamp":1765369737577,"version":"3.40.5"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2020,12,31]],"date-time":"2020-12-31T00:00:00Z","timestamp":1609372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia and FEDER","award":["(POCI-01-0145-FEDER-029628)"],"award-info":[{"award-number":["(POCI-01-0145-FEDER-029628)"]}]},{"name":"Ministerio de Econom\u00eda y Competitividad (Gobierno de Espa\u00f1a) and FEDER","award":["PI16\/01477"],"award-info":[{"award-number":["PI16\/01477"]}]},{"name":"Conseller\u00eda de Cultura, Educaci\u00f3n e Ordenaci\u00f3n Universitaria, (Xunta de Galicia) and FEDER","award":["ED431C2017\/57"],"award-info":[{"award-number":["ED431C2017\/57"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["UIDB\/AGR\/04033\/2020"],"award-info":[{"award-number":["UIDB\/AGR\/04033\/2020"]}]},{"name":"European Regional Development Fund under the scope of Norte2020","award":["NORTE-01-0145-FEDER-000004"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000004"]}]},{"name":"European Regional Development Fund under the scope of Norte2020","award":["UIDB\/04469\/2020"],"award-info":[{"award-number":["UIDB\/04469\/2020"]}]},{"name":"Secretar\u00eda General de Universidades, Spanish Ministerio de Educaci\u00f3n, Cultura y Deporte","award":["FPU15\/02644"],"award-info":[{"award-number":["FPU15\/02644"]}]},{"name":"Secretar\u00eda General de Universidades, Spanish Ministerio de Educaci\u00f3n, Cultura y Deporte","award":["ED481A-2015\/149"],"award-info":[{"award-number":["ED481A-2015\/149"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The prevalence of Shiga toxin (Stx)-producing <jats:italic>Escherichia coli<\/jats:italic> (STEC) was determined by evaluating its presence in faecal samples from 155 heifers, and 254 dairy cows in 21 farms at North of Portugal sampled between December 2017 and June 2019. The prevalence of STEC in heifers (45%) was significantly higher than in lactating cows (16%) (<jats:italic>p<\/jats:italic>&lt;0.05, Fisher exact test statistic value is &lt;0.00001). A total of 133 STEC were isolated, 24 (13.8%) carried Shiga-toxin 1 (<jats:italic>stx1<\/jats:italic>) genes, 69 (39.7%) carried Shiga-toxin 2 (<jats:italic>stx2<\/jats:italic>) genes, and 40 (23%) carried both <jats:italic>stx1<\/jats:italic> and <jats:italic>stx2<\/jats:italic>. Intimin (<jats:italic>eae<\/jats:italic>) virulence gene was detected in 29 (21.8%) of the isolates. STEC isolates belonged to 72 different O:H serotypes, comprising 40 O serogroups and 23 H types. The most frequent serotypes were O29:H12 (15%) and O113:H21 (5.2%), found in a large number of farms. Two isolates belonged to the highly virulent serotypes associated with human disease O157:H7 and O26:H11. Many other bovine STEC serotypes founded in this work belonged to serotypes previously described as pathogenic to humans. Thus, this study highlights the need for control strategies that can reduce STEC prevalence at the farm level and, thus, prevent food and environmental contamination.<\/jats:p>","DOI":"10.1371\/journal.pone.0244713","type":"journal-article","created":{"date-parts":[[2020,12,31]],"date-time":"2020-12-31T19:46:09Z","timestamp":1609443969000},"page":"e0244713","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":24,"title":["Prevalence and serotypes of Shiga toxin-producing Escherichia coli (STEC) in dairy cattle from Northern Portugal"],"prefix":"10.1371","volume":"15","author":[{"given":"Andressa","family":"Ballem","sequence":"first","affiliation":[]},{"given":"Soraia","family":"Gon\u00e7alves","sequence":"additional","affiliation":[]},{"given":"Isidro","family":"Garcia-Meni\u00f1o","sequence":"additional","affiliation":[]},{"given":"Saskia 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