{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:21:28Z","timestamp":1790968888682,"version":"4.1.0"},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T00:00:00Z","timestamp":1619049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fukui Prefectural University","award":["02R-02-02-2 (Strategic Research Promotion Grant)"],"award-info":[{"award-number":["02R-02-02-2 (Strategic Research Promotion Grant)"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Dermal fibroblast aging contributes to aging-associated functional defects in the skin since dermal fibroblasts maintain skin homeostasis by interacting with the epidermis and extracellular matrix. Here, we found that puerarin, an isoflavone present in\n                    <jats:italic>Pueraria lobata<\/jats:italic>\n                    (Kudzu), can prevent the development of the aging-phenotype in human dermal fibroblasts. Normal human dermal fibroblasts (NHDFs) were subcultivated and high-passage cells were selected as senescent cells, whereas low-passage cells were selected as a young cell control. Puerarin treatment increased cell proliferation and decreased the proportion of senescence-associated beta-galactosidase-positive cells in a high-passage culture of NHDFs. Moreover, puerarin treatment reduced the number of smooth muscle actin (SMA)-positive myofibroblasts and the expression of a reticular fibroblast marker, calponin 1 (CNN1), which were induced in high-passage NHDFs. Fulvestrant, an estrogen receptor antagonist, blocked the puerarin-mediated downregulation of SMA and CNN1. Our results suggest that puerarin may be a useful functional food that alleviates aging-related functional defects in dermal fibroblasts.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0249367","type":"journal-article","created":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T15:57:29Z","timestamp":1619107049000},"page":"e0249367","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["Puerarin blocks the aging phenotype in human dermal fibroblasts"],"prefix":"10.1371","volume":"16","author":[{"given":"Yuki","family":"Kamiya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mao","family":"Odama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aki","family":"Mizuguti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeru","family":"Murakami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1023-587X","authenticated-orcid":true,"given":"Takashi","family":"Ito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,4,22]]},"reference":[{"key":"pone.0249367.ref001","first-page":"729","volume-title":"Cell Transplantation","author":"S Zhang","year":"2018"},{"key":"pone.0249367.ref002","first-page":"298","volume-title":"Dermato-Endocrinology","author":"SK Schagen","year":"2012"},{"key":"pone.0249367.ref003","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/s12603-016-0736-8","article-title":"Nutritional status and habitual dietary intake are associated with frail skin conditions in community-dwelling older people","volume":"21","author":"S Iizaka","year":"2017","journal-title":"J Nutr Heal Aging"},{"key":"pone.0249367.ref004","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1016\/j.jaad.2018.03.033","article-title":"A healthy diet in women is associated with less facial wrinkles in a large Dutch population-based cohort","volume":"80","author":"S Meki\u0107","year":"2019","journal-title":"J Am Acad Dermatol"},{"key":"pone.0249367.ref005","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41514-018-0023-5","article-title":"Blocking negative effects of senescence in human skin fibroblasts with a plant extract","volume":"4","author":"I L\u00e4mmermann","year":"2018","journal-title":"npj Aging Mech Dis"},{"key":"pone.0249367.ref006","first-page":"1","volume-title":"Mechanisms of Ageing and Development","author":"W Li","year":"2019"},{"key":"pone.0249367.ref007","first-page":"1","article-title":"Biomarkers of cellular senescence and skin aging","volume":"9","author":"AS Wang","year":"2018","journal-title":"Front Genet"},{"key":"pone.0249367.ref008","first-page":"1194","article-title":"The hallmarks of aging","author":"C L\u00f3pez-Ot\u00edn","year":"2013","journal-title":"Cell. 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