{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T09:54:34Z","timestamp":1774778074976,"version":"3.50.1"},"reference-count":92,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T00:00:00Z","timestamp":1665964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["SFRH\/BDE\/103606\/2014"],"award-info":[{"award-number":["SFRH\/BDE\/103606\/2014"]}]},{"name":"Association BLC3 - Technology and Innovation Campus","award":["UID\/ 05083\/2020"],"award-info":[{"award-number":["UID\/ 05083\/2020"]}]},{"name":"Centre for Functional Ecology - Science for People & the Planet","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}]},{"name":"Center for Neuroscience and Cell Biology","award":["UIDB\/04539\/2020"],"award-info":[{"award-number":["UIDB\/04539\/2020"]}]},{"name":"Centro Portugal Regional Operational Program","award":["CENTRO-04-3559-FSE-000146"],"award-info":[{"award-number":["CENTRO-04-3559-FSE-000146"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Despite the growing interest in the ruminants\u2019 gastrointestinal tract (GIT) microbiomes\u2019 ability to degrade plant materials by animal husbandry and industrial sectors, only a few studies addressed browsing ruminants. The present work describes the taxonomic and functional profile of the bacterial and archaeal communities from five different gastrointestinal sections (rumen, omasum-abomasum, jejunum, cecum and colon) of browsing <jats:italic>Capra hircus<\/jats:italic>, by metabarcoding using 16S rRNA genes hypervariable regions. The bacterial communities across the GITs are mainly composed of <jats:italic>Bacillota<\/jats:italic> and <jats:italic>Bacteroidota<\/jats:italic>. <jats:italic>Prevotella<\/jats:italic> was the leading bacterial group found in the stomachs, <jats:italic>Romboutsia<\/jats:italic> in the jejuna, and <jats:italic>Rikenellaceae_RC9_gut_group<\/jats:italic>, <jats:italic>Bacteroides<\/jats:italic>, <jats:italic>UCG-010_ge<\/jats:italic>, <jats:italic>UCG-005<\/jats:italic>, and <jats:italic>Alistipes<\/jats:italic> in large intestines. The archaeal communities in the stomachs and jejuna revealed to be mainly composed of <jats:italic>Methanobrevibacter<\/jats:italic>, while in the large intestines its dominance is shared with <jats:italic>Methanocorpusculum<\/jats:italic>. Across the GITs, the main metabolic functions were related to carbohydrate, amino acid, and energy metabolisms. Significant differences in the composition and potential biological functions of the bacterial communities were observed among stomachs, jejuna and large intestines. In contrast, significant differences were observed among stomachs and jejuna verse large intestines for archaeal communities. Overall different regions of the GIT are occupied by different microbial communities performing distinct biological functions. A high variety of glycoside hydrolases (GHs) indispensable for degrading plant cell wall materials were predicted to be present in all the GIT sections.<\/jats:p>","DOI":"10.1371\/journal.pone.0276262","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T17:29:58Z","timestamp":1666027798000},"page":"e0276262","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":26,"title":["The gastrointestinal microbiome of browsing goats (Capra hircus)"],"prefix":"10.1371","volume":"17","author":[{"given":"Vera","family":"Guerra","sequence":"first","affiliation":[]},{"given":"Igor","family":"Tiago","sequence":"additional","affiliation":[]},{"given":"Aitana","family":"Aires","sequence":"additional","affiliation":[]},{"given":"Catarina","family":"Coelho","sequence":"additional","affiliation":[]},{"given":"Jo\u00e3o","family":"Nunes","sequence":"additional","affiliation":[]},{"given":"L\u00edgia O.","family":"Martins","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4996-3185","authenticated-orcid":true,"given":"Ant\u00f3nio","family":"Ver\u00edssimo","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2022,10,17]]},"reference":[{"issue":"1","key":"pone.0276262.ref001","doi-asserted-by":"crossref","first-page":"145","DOI":"10.2527\/2006.841145x","article-title":"Relationships of feedlot feed efficiency, performance, and feeding behavior with metabolic rate, methane production, and energy partitioning in beef cattle","volume":"84","author":"JD Nkrumah","year":"2006","journal-title":"J Anim Sci"},{"issue":"4","key":"pone.0276262.ref002","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.2527\/jas.2010-3460","article-title":"Ruminant Nutrition Symposium: Productivity, digestion, and health responses to hindgut acidosis in ruminants","volume":"89","author":"TF Gressley","year":"2011","journal-title":"J Anim Sci"},{"issue":"2","key":"pone.0276262.ref003","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.rvsc.2012.09.007","article-title":"Changes in methane emission, rumen fermentation in response to diet and microbial interactions","volume":"94","author":"S Kumar","year":"2013","journal-title":"Res Vet Sci"},{"issue":"1","key":"pone.0276262.ref004","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-021-88943-9","article-title":"Characterizing rumen microbiota and CAZyme profile of Indian dromedary camel (Camelus dromedarius) in response to different roughages","volume":"11","author":"AT Hinsu","year":"2021","journal-title":"Sci Rep"},{"issue":"11","key":"pone.0276262.ref005","doi-asserted-by":"crossref","first-page":"e78507","DOI":"10.1371\/journal.pone.0078507","article-title":"Metagenomic insights into the carbohydrate-active enzymes carried by the microorganisms adhering to solid digesta in the rumen of cows","volume":"8","author":"L Wang","year":"2013","journal-title":"PLoS One"},{"issue":"1","key":"pone.0276262.ref006","first-page":"15","article-title":"Exploring the sheep rumen microbiome for carbohydrate-active enzymes. 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