{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T03:36:10Z","timestamp":1775619370786,"version":"3.50.1"},"reference-count":22,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T00:00:00Z","timestamp":1713398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BEX\/BID\/5410\/2014"],"award-info":[{"award-number":["PTDC\/BEX\/BID\/5410\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BEX\/BID\/5410\/2014"],"award-info":[{"award-number":["PTDC\/BEX\/BID\/5410\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/146043\/2019"],"award-info":[{"award-number":["SFRH\/BD\/146043\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["2021.00238.CEECIND"],"award-info":[{"award-number":["2021.00238.CEECIND"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["RISE - LA\/P\/0053\/2020"],"award-info":[{"award-number":["RISE - LA\/P\/0053\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"AD-ABC, Associa\u00e7\u00e3o para o Desenvolvimento do Centro Acad\u00e9mico de Investiga\u00e7\u00e3o e Forma\u00e7\u00e3o Biom\u00e9dica do Algarve"},{"name":"AD-ABC, Associa\u00e7\u00e3o para o Desenvolvimento do Centro Acad\u00e9mico de Investiga\u00e7\u00e3o e Forma\u00e7\u00e3o Biom\u00e9dica do Algarve"},{"name":"Munic\u00edpio de Loul\u00e9"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    During vertebrate embryo development, the body is progressively segmented along the anterior-posterior (A-P) axis early in development. The rate of somite formation is controlled by the somitogenesis embryo clock (EC), which was first described as gene expression oscillations of\n                    <jats:italic>hairy1<\/jats:italic>\n                    (\n                    <jats:italic>hes4<\/jats:italic>\n                    ) in the presomitic mesoderm of chick embryos with 15\u201320 somites. Here, the EC displays the same periodicity as somite formation, 90 min, whereas the posterior-most somites (44\u201352) only arise every 150 minutes, matched by a corresponding slower pace of the EC. Evidence suggests that the rostral-most somites are formed faster, however, their periodicity and the EC expression dynamics in these early stages are unknown. In this study, we used time-lapse imaging of chicken embryos from primitive streak to somitogenesis stages with high temporal resolution (3-minute intervals). We measured the length between the anterior-most and the last formed somitic clefts in each captured frame and developed a simple algorithm to automatically infer both the length and time of formation of each somite. We found that the occipital somites (up to somite 5) form at an average rate of 75 minutes, while somites 6 onwards are formed approximately every 90 minutes. We also assessed the expression dynamics of\n                    <jats:italic>hairy1<\/jats:italic>\n                    using half-embryo explants cultured for different periods of time. This showed that EC\n                    <jats:italic>hairy1<\/jats:italic>\n                    expression is highly dynamic prior to somitogenesis and assumes a clear oscillatory behaviour as the first somites are formed. Importantly, using\n                    <jats:italic>ex ovo<\/jats:italic>\n                    culture and live-imaging techniques, we showed that the\n                    <jats:italic>hairy1<\/jats:italic>\n                    expression pattern recapitulates with the formation of each new pair of somites, indicating that somite segmentation is coupled with EC oscillations since the onset of somitogenesis.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0297853","type":"journal-article","created":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T13:26:56Z","timestamp":1713446816000},"page":"e0297853","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Spatio-temporal dynamics of early somite segmentation in the chicken embryo"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5086-2632","authenticated-orcid":true,"given":"Ana Cristina","family":"Maia-Fernandes","sequence":"first","affiliation":[]},{"given":"Ana","family":"Martins-Jesus","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5713-3240","authenticated-orcid":true,"given":"N\u00edsia","family":"Borralho-Martins","sequence":"additional","affiliation":[]},{"given":"Tom\u00e1s","family":"Pais-de-Azevedo","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5226-3441","authenticated-orcid":true,"given":"Ramiro","family":"Magno","sequence":"additional","affiliation":[]},{"given":"Isabel","family":"Duarte","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0397-5917","authenticated-orcid":true,"given":"Raquel P.","family":"Andrade","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2024,4,18]]},"reference":[{"key":"pone.0297853.ref001","first-page":"381","article-title":"Early stages of chick somite 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