{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T21:15:45Z","timestamp":1757625345638,"version":"3.44.0"},"reference-count":61,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T00:00:00Z","timestamp":1757376000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Uropathogenic <jats:italic>Escherichia coli<\/jats:italic> (UPEC) are among the first pathogens to colonise in catheter and non-catheter-associated urinary tract infections. However, these infections are often polymicrobial, resulting in multi-species infections that persist by forming biofilms. Living within these highly antimicrobial tolerant communities, bacteria can establish intra- and inter-specific interactions, including quorum sensing (QS)-mediated signalling mechanisms, which play a key role in biofilm establishment and maturation. Although <jats:italic><jats:italic>E. coli<\/jats:italic><\/jats:italic> does not produce <jats:italic><jats:italic>N<\/jats:italic><\/jats:italic>-acylhomoserine lactones (AHLs), it possesses an orphan LuxR-type receptor, SdiA, which can bind these QS signals released by other Gram-negative bacteria, modulating several virulence-associated phenotypes including biofilm formation. Despite biofilms being considered a major public health challenge due to their persistence and resilience, the knowledge of the SdiA role in biofilm regulation and UPEC fitness in mixed biofilms is limited compared to enteropathogenic <jats:italic><jats:italic>E. coli<\/jats:italic><\/jats:italic>. We have used a \u0394<jats:italic><jats:italic>sdiA<\/jats:italic><\/jats:italic> mutant and phenotypic analysis to investigate the SdiA influence on UPEC single and mixed biofilms with <jats:italic><jats:italic>Pseudomonas aeruginosa<\/jats:italic><\/jats:italic>. SdiA was found to inhibit UPEC biofilm and addition of AHLs enhanced <jats:italic><jats:italic>E. coli<\/jats:italic><\/jats:italic> surface colonisation via SdiA-mediated de-repression of biofilm. We also confirmed the low specificity of SdiA for AHLs, demonstrating the SdiA importance in tightly regulating the UPEC free-living and biofilm-associated lifestyles.<\/jats:p>","DOI":"10.1371\/journal.pone.0328837","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T17:21:57Z","timestamp":1757438517000},"page":"e0328837","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["AHL-based QS signalling promotes uropathogenic Escherichia coli settlement through the de-repression of biofilm formation by SdiA"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6981-6601","authenticated-orcid":true,"given":"Celia","family":"Mayer","sequence":"first","affiliation":[]},{"given":"Fadi","family":"Soukarieh","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3355-4398","authenticated-orcid":true,"given":"Manuel","family":"Sim\u00f5es","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0933-0850","authenticated-orcid":true,"given":"Saskia-Camille","family":"Flament-Simon","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4585-7385","authenticated-orcid":true,"given":"Miguel","family":"C\u00e1mara","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6902-6776","authenticated-orcid":true,"given":"Manuel","family":"Romero","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2025,9,9]]},"reference":[{"issue":"2","key":"pone.0328837.ref001","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/nrmicro818","article-title":"Pathogenic Escherichia coli","volume":"2","author":"JB Kaper","year":"2004","journal-title":"Nat Rev Microbiol"},{"issue":"3","key":"pone.0328837.ref002","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1128\/CMR.00125-13","article-title":"Escherichia coli ST131, an intriguing clonal group","volume":"27","author":"M-H Nicolas-Chanoine","year":"2014","journal-title":"Clin Microbiol Rev"},{"issue":"1","key":"pone.0328837.ref003","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/s41579-020-0416-x","article-title":"The population genetics of pathogenic Escherichia coli","volume":"19","author":"E Denamur","year":"2020","journal-title":"Nat Rev Microbiol"},{"key":"pone.0328837.ref004","article-title":"Disease burden and long-term trends of urinary tract infections: A worldwide report","volume":"10","author":"X Yang","year":"2022","journal-title":"Front Public Health"},{"issue":"1","key":"pone.0328837.ref005","doi-asserted-by":"crossref","first-page":"4702","DOI":"10.1038\/s41598-025-89240-5","article-title":"Epidemiological trends and predictions of urinary tract infections in the global burden of disease study 2021","volume":"15","author":"Y He","year":"2025","journal-title":"Sci Rep"},{"issue":"2","key":"pone.0328837.ref006","doi-asserted-by":"crossref","DOI":"10.1128\/ecosal.8.6.1.3","article-title":"Uropathogenic Escherichia coli","volume":"3","author":"HLT Mobley","year":"2009","journal-title":"EcoSal Plus"},{"issue":"5","key":"pone.0328837.ref007","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1128\/JB.00985-13","article-title":"Type 1 fimbriae contribute to catheter-associated urinary tract infections caused by Escherichia coli","volume":"196","author":"A Reisner","year":"2014","journal-title":"J Bacteriol"},{"issue":"4","key":"pone.0328837.ref008","doi-asserted-by":"crossref","first-page":"65","DOI":"10.3390\/pathogens5040065","article-title":"Microbial Biofilms in Urinary Tract Infections and Prostatitis: Etiology, Pathogenicity, and Combating strategies","volume":"5","author":"C Delcaru","year":"2016","journal-title":"Pathogens"},{"key":"pone.0328837.ref009","unstructured":"World Health Organization. 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