{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T11:34:41Z","timestamp":1767180881341,"version":"build-2238731810"},"update-to":[{"DOI":"10.1371\/journal.ppat.1011003","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T00:00:00Z","timestamp":1674000000000}}],"reference-count":70,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T00:00:00Z","timestamp":1672876800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["STE 2348\/2-1 and STE 2348\/2-2"],"award-info":[{"award-number":["STE 2348\/2-1 and STE 2348\/2-2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001655","name":"German Academic Exchange Service","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001655","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Medical Faculty of the University of Freiburg","award":["2021\/A3-Sch"],"award-info":[{"award-number":["2021\/A3-Sch"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    (\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    ) is a zoonotic apicomplexan parasite that is an important cause of clinical disability in humans. On a global scale, one third of the human population is infected with\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    . Mice and other small rodents are believed to be responsible for transmission of\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    to the domestic cat, its definitive host. Interferon-inducible Immunity-Related GTPases (IRG proteins) are important for control of murine\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    infections. Virulence differences between\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    strains are linked to polymorphic rhoptry proteins (ROPs) that cooperate to inactivate individual IRG family members. In particular, the pseudokinase ROP5 isoform B is critically important in laboratory strains of mice. We identified\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    ROP39 in complex with ROP5B and demonstrate its contribution to acute\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    virulence. ROP39 directly targets Irgb10 and inhibits homodimer formation of the GTPase leading to an overall reduction of IRG protein loading onto the parasitophorous vacuolar membrane (PVM). Maintenance of PVM integrity rescues the parasite from IRG protein-mediated clearance\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    . This study identifies a novel\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    effector that is important for specific inactivation of the IRG resistance system. Our data reveal that yet unknown\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    effectors can emerge from identification of direct interaction partners of ROP5B.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1011003","type":"journal-article","created":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T14:00:57Z","timestamp":1672927257000},"page":"e1011003","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["ROP39 is an Irgb10-specific parasite effector that modulates acute Toxoplasma gondii virulence"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8488-3992","authenticated-orcid":true,"given":"Shishir","family":"Singh","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3462-3612","authenticated-orcid":true,"given":"Mateo","family":"Murillo-Le\u00f3n","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5093-1020","authenticated-orcid":true,"given":"Niklas Sebastian","family":"Endres","sequence":"additional","affiliation":[]},{"given":"Ailan Farid","family":"Arenas Soto","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6472-3329","authenticated-orcid":true,"given":"Jorge Enrique","family":"G\u00f3mez-Mar\u00edn","sequence":"additional","affiliation":[]},{"given":"Florence","family":"Melbert","sequence":"additional","affiliation":[]},{"given":"Thirumala-Devi","family":"Kanneganti","sequence":"additional","affiliation":[]},{"given":"Masahiro","family":"Yamamoto","sequence":"additional","affiliation":[]},{"given":"Claudia","family":"Campos","sequence":"additional","affiliation":[]},{"given":"Jonathan Charles","family":"Howard","sequence":"additional","affiliation":[]},{"given":"Gregory Alan","family":"Taylor","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1975-5513","authenticated-orcid":true,"given":"Tobias","family":"Steinfeldt","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2023,1,5]]},"reference":[{"issue":"9","key":"ppat.1011003.ref001","doi-asserted-by":"crossref","first-page":"e3182","DOI":"10.1371\/journal.pntd.0003182","article-title":"Geographic separation of 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