{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T12:53:28Z","timestamp":1770987208167,"version":"3.50.1"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2003,12,1]],"date-time":"2003-12-01T00:00:00Z","timestamp":1070236800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Background: To separate non-(1\u201384)parathyroid hormone [non-(1\u201384)PTH] from PTH(1\u201384), we developed new HPLC gradients and observed that the peak coeluting with hPTH(1\u201384) could be separated into two entities recognized by a cyclase-activating PTH (CA-PTH) assay that reacts with the first four amino acids of the PTH structure.<\/jats:p><jats:p>Methods: Sera from six healthy individuals and five patients with primary hyperparathyroidism, and eight pools of sera from patients in renal failure were fractionated by HPLC. A total (T)-PTH assay reacting with the (15\u201320) region, the CA-PTH assay, and a COOH-terminal (C)-PTH assay with a (65\u201384) structure requirement were used to measure basal and fractionated PTH values.<\/jats:p><jats:p>Results: T-PTH was higher than CA-PTH in all healthy controls [mean (SD), 3.13 (0.37) vs 2.29 (0.33) pmol\/L; P &amp;lt;0.01] and in renal failure patients [47 (35.1) vs 33.4 (26.1) pmol\/L; P &amp;lt;0.01]. By contrast, CA-PTH concentrations were similar to or higher than T-PTH in three of five patients with primary hyperparathyroidism [25.7 (26.1) vs 23.1 (24.2) pmol\/L; not significant]. The CA-PTH assay reacted with the hPTH(1\u201384) peak and with a minor peak different from the non-(1\u201384) peak recognized by the T-PTH assay. This minor peak was not recognized by the T-PTH assay. It represented 8 (2)% of CA-PTH in controls, 25 (23)% in patients with primary hyperparathyroidism, and 22 (7)% in renal failure patients, assuming equimolar reactivity to hPTH(1\u201384) in the CA-PTH assay. It was not oxidized hPTH(1\u201384), which migrated differently on HPLC and reacted similarly in the CA and T-PTH assays.<\/jats:p><jats:p>Conclusions: This new molecular form of PTH has structural integrity of the (1\u20134) region but presumably is modified in the region (15\u201320), which is usually recognized by the T-PTH assay. Its clinical implications remain to be defined.<\/jats:p>","DOI":"10.1373\/clinchem.2003.021592","type":"journal-article","created":{"date-parts":[[2003,12,10]],"date-time":"2003-12-10T21:51:05Z","timestamp":1071093065000},"page":"2037-2044","source":"Crossref","is-referenced-by-count":103,"title":["Amino-Terminal Form of Parathyroid Hormone (PTH) with Immunologic Similarities to hPTH(1\u201384) Is Overproduced in Primary and Secondary Hyperparathyroidism"],"prefix":"10.1093","volume":"49","author":[{"given":"Pierre","family":"D\u2019Amour","sequence":"first","affiliation":[{"name":"Author de Recherche, Centre Hospitalier de l\u2019Universite\u0301 de Montre\u0301al (CHUM), Ho\u0302pital Saint-Luc, and De\u0301partement de Me\u0301decine, Universite\u0301 de Montre\u0301al, Montre\u0301al, Que\u0301bec H2X 1P1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Hugues","family":"Brossard","sequence":"first","affiliation":[{"name":"Author de Recherche, Centre 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