{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T14:05:16Z","timestamp":1773151516011,"version":"3.50.1"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Background: Non-(1\u201384) parathyroid hormone (PTH) fragments are large C-terminal fragments of PTH with a partially preserved N-terminal structure. They differ from other C-terminal PTH fragments, which do not have an N-terminal structure and do not react in intact PTH assays. We aimed to identify the minimal N-terminal structure common to all non-(1\u201384) PTH fragments.<\/jats:p><jats:p>Methods: Sera obtained from six healthy individuals and six patients with primary hyperparathyroidism, and six serum pools from dialysis patients with different PTH concentrations were fractionated by HPLC and analyzed by four different PTH assays. Each assay was characterized by saturation analysis of its detection antibody and capacity to react with different PTH fragments. Human PTH(1\u201384) [hPTH(1\u201384)] calibrators were normalized to an in-house hPTH(1\u201384) calibrator.<\/jats:p><jats:p>Results: The cyclase-activating PTH (CA-PTH) assay had an early (1, 2,) epitope and reacted only with hPTH(1\u201384). The other assays had epitopes in region (13\u201334). Total and intact PTH assays had epitopes proximal to position 18 and reacted equally well with hPTH(1\u201384) and hPTH(7\u201384), and the Elecsys PTH assay had an epitope distal to position 19, being saturable by hPTH(18\u201348) and also reacting with [Tyr34]hPTH(19\u201384). The HPLC profiles obtained with these assays showed that non-(1\u201384) PTH fragments did not react in the CA-PTH assay, as expected. The amount of non-(1\u201384) PTH detected by the other three assays was similar when the assay results were normalized to a common calibrator.<\/jats:p><jats:p>Conclusions: The results suggest that the amount of non-(1\u201384) PTH detected by epitopes proximal or distal to position 19 of the PTH structure is identical, indicating a common minimum structure starting before position 19. This in turn points to a probable high-affinity interaction with the C-PTH receptor, as observed previously with [Tyr34]hPTH(19\u201384) in various cell lines and in mouse osteocytes with PTH\/PTHrP type I receptor ablation.<\/jats:p>","DOI":"10.1373\/clinchem.2004.040485","type":"journal-article","created":{"date-parts":[[2004,10,29]],"date-time":"2004-10-29T00:25:54Z","timestamp":1099009554000},"page":"169-176","source":"Crossref","is-referenced-by-count":50,"title":["Evidence That the Amino-Terminal Composition of Non-(1\u201384) Parathyroid Hormone Fragments Starts before Position 19"],"prefix":"10.1093","volume":"51","author":[{"given":"Pierre","family":"D\u2019Amour","sequence":"first","affiliation":[{"name":"Centre de Recherche, Centre Hospitalier de l\u2019Universite\u0301 de Montre\u0301al (CHUM), Ho\u0302pital Saint-Luc, and Departments of"},{"name":"Medicine and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Hugues","family":"Brossard","sequence":"first","affiliation":[{"name":"Centre de Recherche, Centre Hospitalier de l\u2019Universite\u0301 de Montre\u0301al (CHUM), Ho\u0302pital Saint-Luc, and Departments of"},{"name":"Medicine and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agne\u0300s","family":"Ra\u0308kel","sequence":"first","affiliation":[{"name":"Centre de Recherche, Centre Hospitalier de l\u2019Universite\u0301 de Montre\u0301al (CHUM), Ho\u0302pital Saint-Luc, and Departments of"},{"name":"Medicine and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louise","family":"Rousseau","sequence":"first","affiliation":[{"name":"Centre de Recherche, Centre Hospitalier de l\u2019Universite\u0301 de Montre\u0301al (CHUM), Ho\u0302pital Saint-Luc, and Departments of"},{"name":"Medicine and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Albert","sequence":"first","affiliation":[{"name":"Centre de Recherche, Centre Hospitalier de l\u2019Universite\u0301 de Montre\u0301al (CHUM), Ho\u0302pital Saint-Luc, and Departments of"},{"name":"Biochemistry, Ho\u0302pital Saint-Luc and Universite\u0301 de Montre\u0301al, Montre\u0301al, Que\u0301bec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Cantor","sequence":"first","affiliation":[{"name":"Scantibodies Laboratory Inc., Santee, CA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2005,1,1]]},"reference":[{"key":"2020022800541849100_R1","doi-asserted-by":"crossref","unstructured":"Brossard JH, Whittom S, Lepage R, D\u2019Amour P. Carboxyl-terminal fragments of parathyroid hormone are not secreted preferentially in primary hyperparathyroidism as they are in other causes of hypercalcemia. J Clin Endocrinol Metab1993;77:413-419.","DOI":"10.1210\/jcem.77.2.8345045"},{"key":"2020022800541849100_R2","doi-asserted-by":"crossref","unstructured":"Brossard JH, Cloutier M, Roy L, Lepage R, Gascon-Barre\u0301 M, D\u2019Amour P. Accumulation of a non-(1\u201384) molecular form detected by intact PTH assay in renal failure: importance in the interpretation of PTH values. J Clin Endocrinol Metab1996;81:3923-3929.","DOI":"10.1210\/jcem.81.11.8923839"},{"key":"2020022800541849100_R3","doi-asserted-by":"crossref","unstructured":"Lepage R, Roy L, Brossard JH, Rousseau L, Dorais C, Lazure C, et al. A non-(1\u201384) circulating parathyroid hormone (PTH) fragment interferes significantly with intact PTH commercial assay measurements in uremic samples. 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