{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T08:32:22Z","timestamp":1771489942433,"version":"3.50.1"},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2005,7,1]],"date-time":"2005-07-01T00:00:00Z","timestamp":1120176000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Background: Extraction protocols using magnetic solid phases offer a high potential for automation. However, commercially available magnetic-bead\u2013based assays either lack the sensitivity required for viral diagnostics or are disproportionately expensive.<\/jats:p>\n               <jats:p>Methods: We developed an aqueous chemistry for extraction of viral nucleic acids from plasma samples by use of common magnetic silica beads. Nucleic acids were bound to the beads at acidic conditions in the presence of a kosmotropic salt and were eluted at a slightly alkaline pH. The method was implemented on a standard pipetting workstation for fully automated extraction of up to 48 samples of 240 \u03bcL plasma in 1 batch.<\/jats:p>\n               <jats:p>Results: The detection limit of the method was comparable to the spin-column\u2013based QIAamp Viral RNA Mini Kit, which relies on chaotropic salts and binding to a silica membrane, as the comparison method. The 95% detection limit was 23.1 IU per PCR for HIV-1 and 10.7 IU per PCR for hepatitis C virus (HCV). Suitability for clinical routine testing was confirmed in a total of 178 HIV-1- or HCV-positive plasma samples. The method linearity (R2) was &amp;gt;0.99 for the viruses evaluated.<\/jats:p>\n               <jats:p>Conclusions: Use of reagents without organic solvents allows simple and cost-effective automation of this method on common pipetting robots with low risk of contamination. Performance characteristics of the novel extraction method make it suitable for use in diagnosis of infectious diseases and viral load determinations.<\/jats:p>","DOI":"10.1373\/clinchem.2005.048603","type":"journal-article","created":{"date-parts":[[2005,6,23]],"date-time":"2005-06-23T17:23:28Z","timestamp":1119547408000},"page":"1217-1222","source":"Crossref","is-referenced-by-count":32,"title":["High-Throughput Purification of Viral RNA Based on Novel Aqueous Chemistry for Nucleic Acid Isolation"],"prefix":"10.1093","volume":"51","author":[{"given":"Michael K","family":"Hourfar","sequence":"first","affiliation":[{"name":"Institute of Transfusion Medicine and Immunohaematology, German Red Cross, Johann Wolfgang Goethe University, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uwe","family":"Michelsen","sequence":"first","affiliation":[{"name":"Merck KGaA, Darmstadt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Schmidt","sequence":"first","affiliation":[{"name":"Institute of Transfusion Medicine and Immunohaematology, German Red Cross, Johann Wolfgang Goethe University, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annemarie","family":"Berger","sequence":"first","affiliation":[{"name":"Institute for Medical Virology, Johann Wolfgang Goethe University Hospital, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erhard","family":"Seifried","sequence":"first","affiliation":[{"name":"Institute of Transfusion Medicine and Immunohaematology, German Red Cross, Johann Wolfgang Goethe University, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W Kurt","family":"Roth","sequence":"first","affiliation":[{"name":"Institute of Transfusion Medicine and Immunohaematology, German Red Cross, Johann Wolfgang Goethe University, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2005,7,1]]},"reference":[{"key":"2020022800550325700_R1","doi-asserted-by":"crossref","unstructured":"Marshall DA, Kleinman SH, Wong JB, AuBuchon JB, Grima DT, Kulin NA, et al. 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