{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T09:14:39Z","timestamp":1775121279525,"version":"3.50.1"},"reference-count":12,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2006,5,1]],"date-time":"2006-05-01T00:00:00Z","timestamp":1146441600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Background: Although a homogeneous assay for serum LDL-cholesterol (LDL-C) has become a routine clinical procedure, problems remain in assay performance characteristics.<\/jats:p><jats:p>Methods: We examined the performance of a recently developed automated homogeneous assay (New-Daiichi assay) for serum LDL-C and compared the results with those obtained by the current homogeneous method (Denka-Seiken assay) or by ultracentrifugation as a control.<\/jats:p><jats:p>Results: The New-Daiichi assay showed satisfactory basic performance characteristics such as reproducibility, linearity, and stability. There was no interference in the assay by various substances examined. The LDL-C values obtained with this method correlated well with those obtained by ultracentrifugation. In samples from patients with obstructive jaundice, both methods detected cholesterol from abnormal lipoproteins (such as lipoprotein-X and -Y), but the New-Daiichi assay was less reactive and more specific for LDL-C.<\/jats:p><jats:p>Conclusion: The new method has improved performance for the accurate measurement of LDL-C in clinical practice.<\/jats:p>","DOI":"10.1373\/clinchem.2005.053983","type":"journal-article","created":{"date-parts":[[2006,3,24]],"date-time":"2006-03-24T01:44:21Z","timestamp":1143164661000},"page":"886-888","source":"Crossref","is-referenced-by-count":11,"title":["Improved Specificity of a New Homogeneous Assay for LDL-Cholesterol in Serum with Abnormal Lipoproteins"],"prefix":"10.1093","volume":"52","author":[{"given":"Yasumasa","family":"Iwasaki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Matsuyama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuo","family":"Nakashima","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2006,5,1]]},"reference":[{"key":"2020022801231097700_R1","doi-asserted-by":"crossref","unstructured":"Kannel WB, Castelli WP, Gordon T. Cholesterol in the prediction of atherosclerotic disease: new perspectives based on the Framingham study. Ann Intern Med1979;90:85-91.","DOI":"10.7326\/0003-4819-90-1-85"},{"key":"2020022801231097700_R2","doi-asserted-by":"crossref","unstructured":". National Cholesterol Education Program. Second report of the Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel II). Circulation1994;89:1333-1445.","DOI":"10.1161\/01.CIR.89.3.1333"},{"key":"2020022801231097700_R3","doi-asserted-by":"crossref","unstructured":". Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults. Executive Summary of the Third Report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, And Treatment of High Blood Cholesterol In Adults (Adult Treatment Panel III). JAMA2001;285:2486-2497.","DOI":"10.1001\/jama.285.19.2486"},{"key":"2020022801231097700_R4","doi-asserted-by":"crossref","unstructured":". . Randomized trial of cholesterol lowering in 4444 patients with coronary heart disease: the Scandinavian Simvastatin Survival Study (4S). Lancet1994;344:1383-1389.","DOI":"10.1016\/S0140-6736(94)90566-5"},{"key":"2020022801231097700_R5","doi-asserted-by":"crossref","unstructured":"Shepherd J, Cobbe SM, Ford I, Isles CG, Lorimer AR, MacFarlane PW, et al. Prevention of coronary heart disease with pravastatin in men with hypercholesterolemia. West of Scotland Coronary Prevention Study Group. N Engl J Med1995;333:1301-1307.","DOI":"10.1056\/NEJM199511163332001"},{"key":"2020022801231097700_R6","doi-asserted-by":"crossref","unstructured":"Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem1972;18:499-502.","DOI":"10.1093\/clinchem\/18.6.499"},{"key":"2020022801231097700_R7","doi-asserted-by":"crossref","unstructured":"Sakaue T, Hirano T, Yoshino G, Sakai K, Takeuchi H, Adachi M. Reactions of direct LDL-cholesterol assays with pure LDL fraction and IDL: comparison of three homogeneous methods. Clin Chim Acta2000;295:97-106.","DOI":"10.1016\/S0009-8981(00)00200-X"},{"key":"2020022801231097700_R8","doi-asserted-by":"crossref","unstructured":"Nauck M, Rifai N. Analytical performance and clinical efficacy of three routine procedures for LDL cholesterol measurement compared with the ultracentrifugation-dextran sulfate-Mg(2+) method. Clin Chim Acta2000;294:77-92.","DOI":"10.1016\/S0009-8981(99)00250-8"},{"key":"2020022801231097700_R9","doi-asserted-by":"crossref","unstructured":"Usui S, Kakuuchi H, Okamoto M, Mizukami Y, Okazaki M. Differential reactivity of two homogeneous LDL-cholesterol methods to LDL and VLDL subfractions, as demonstrated by ultracentrifugation and HPLC. Clin Chem2002;48:1946-1954.","DOI":"10.1093\/clinchem\/48.11.1946"},{"key":"2020022801231097700_R10","doi-asserted-by":"crossref","unstructured":"Leary ET, Wang T, Baker DJ, Cilla DD, Zhong J, Warnick R, et al. Evaluation of an immunoprecipitation method for quantitative measurement of remnant-like particle-cholesterol in serum and plasma. Clin Chem1998;44:2490-2498.","DOI":"10.1093\/clinchem\/44.12.2490"},{"key":"2020022801231097700_R11","doi-asserted-by":"crossref","unstructured":"Miller JP. Dyslipoproteinaemia of liver disease. Baillieres Clin Endocrinol Metab1990;4:807-832.","DOI":"10.1016\/S0950-351X(05)80080-1"},{"key":"2020022801231097700_R12","doi-asserted-by":"crossref","unstructured":"Fei H, Maeda S, Kirii H, Fujigaki S, Maekawa N, Fujii H, et al. Evaluation of two different homogeneous assays for LDL-cholesterol in lipoprotein-X-positive serum. Clin Chem2000;46:1351-1356.","DOI":"10.1093\/clinchem\/46.9.1351"}],"container-title":["Clinical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/clinchem\/article-pdf\/52\/5\/886\/32686319\/clinchem0886.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/clinchem\/article-pdf\/52\/5\/886\/32686319\/clinchem0886.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,25]],"date-time":"2021-07-25T04:32:03Z","timestamp":1627187523000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/clinchem\/article\/52\/5\/886\/5626892"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5,1]]},"references-count":12,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2006,5,1]]},"published-print":{"date-parts":[[2006,5,1]]}},"URL":"https:\/\/doi.org\/10.1373\/clinchem.2005.053983","relation":{},"ISSN":["0009-9147","1530-8561"],"issn-type":[{"value":"0009-9147","type":"print"},{"value":"1530-8561","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2006,5,1]]},"published":{"date-parts":[[2006,5,1]]}}}