{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T11:21:07Z","timestamp":1768821667082,"version":"3.49.0"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2006,2,1]],"date-time":"2006-02-01T00:00:00Z","timestamp":1138752000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Background: Atrophic body gastritis is considered the first important step in the histogenesis of gastric carcinoma, a multistep process starting from chronic gastritis and progressing through chronic atrophic gastritis, intestinal metaplasia, and dysplasia. Helicobacter pylori is involved in the induction of atrophic body gastritis, but documentation of H. pylori infection is difficult because of the progressive disappearance of the bacterium. Our study aimed to detect past H. pylori infection in patients with atrophic body gastritis.<\/jats:p>\n               <jats:p>Methods: We used Western blot analyses of whole bacterial protein lysate of 2 different strains to probe sera from 143 patients. All sera were analyzed by ELISA (Bio-Rad), and results of gastric histology were available for all patients.<\/jats:p>\n               <jats:p>Results: Among 111 patient sera previously classified as negative for H. pylori infection by ELISA, 106 (95.5%) were positive when assayed by immunoblotting.<\/jats:p>\n               <jats:p>Conclusions: Commercial diagnostic reagent sets may fail to detect H. pylori infection. Western blotting of whole bacterial protein extracts could provide the basis of a noninvasive serology tool able to assess previous infection with H. pylori in patients with atrophic body gastritis.<\/jats:p>","DOI":"10.1373\/clinchem.2005.054627","type":"journal-article","created":{"date-parts":[[2005,11,24]],"date-time":"2005-11-24T01:24:13Z","timestamp":1132795453000},"page":"220-226","source":"Crossref","is-referenced-by-count":19,"title":["Western Blotting of Total Lysate of Helicobacter pylori in Cases of Atrophic Body Gastritis"],"prefix":"10.1093","volume":"52","author":[{"given":"Roberta","family":"Mini","sequence":"first","affiliation":[{"name":"Dipartimento di Biologia Molecolare and Dipartimento di Medicina Interna, Scienze Endocrino-Metaboliche e Biochimica, Policlinico \u201cLe Scotte\u201d, Universita\u0300 degli Studi di Siena, Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Annibale","sequence":"first","affiliation":[{"name":"Digestive and Liver Disease Unit, University \u201cLa Sapienza\u201d, Dipartimento di Scienze Cliniche, Ospedale Sant\u2019Andrea, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edith","family":"Lahner","sequence":"first","affiliation":[{"name":"Digestive and Liver Disease Unit, University \u201cLa Sapienza\u201d, Dipartimento di Scienze Cliniche, Ospedale Sant\u2019Andrea, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Bernardini","sequence":"first","affiliation":[{"name":"Dipartimento di Biologia Molecolare and Dipartimento di Medicina Interna, Scienze Endocrino-Metaboliche e Biochimica, Policlinico \u201cLe Scotte\u201d, Universita\u0300 degli Studi di Siena, Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natale","family":"Figura","sequence":"first","affiliation":[{"name":"Dipartimento di Biologia Molecolare and Dipartimento di Medicina Interna, Scienze Endocrino-Metaboliche e Biochimica, Policlinico \u201cLe Scotte\u201d, Universita\u0300 degli Studi di Siena, Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annalisa","family":"Santucci","sequence":"first","affiliation":[{"name":"Dipartimento di Biologia Molecolare and Dipartimento di Medicina Interna, Scienze Endocrino-Metaboliche e Biochimica, Policlinico \u201cLe Scotte\u201d, Universita\u0300 degli Studi di Siena, Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2006,2,1]]},"reference":[{"key":"2020022801241652300_R1","unstructured":"Lee EL, Feldman M. Gastritis and other gastropathies. Feldman M Friedman LS Sleisenger MH eds. Sleisenger and Fordtran\u2019s gastrointestinal and liver disease: pathophysiology, diagnosis, management, 7th ed2002:810-827 WB Saunders Philadelphia. ."},{"key":"2020022801241652300_R2","unstructured":"Peterson WL, Graham DY. Helicobacter pylori. Feldman M Friedman LS Sleisenger MH eds. Sleisenger and Fordtran\u2019s gastrointestinal and liver disease: pathophysiology, diagnosis, management, 7th ed2002:732-746 WB Saunders Philadelphia. ."},{"key":"2020022801241652300_R3","unstructured":"Correa P. Helicobacter pylori and gastric carcinogenesis. Am J Surg Pathol1995;19(Suppl):S37-S43."},{"key":"2020022801241652300_R4","doi-asserted-by":"crossref","unstructured":"Ming SC. Cellular and molecular pathology of gastric carcinoma and precursor lesions: a critical review. Gastric Cancer1998;1:31-50.","DOI":"10.1007\/s101200050053"},{"key":"2020022801241652300_R5","doi-asserted-by":"crossref","unstructured":"Fong TL, Dooley CP, Dehesa M, Cohen H, Carmel R, Fitzgibbons PL, et al. Helicobacter pylori infection in pernicious anemia: a prospective controlled study. Gastroenterology1991;100:328-332.","DOI":"10.1016\/0016-5085(91)90199-U"},{"key":"2020022801241652300_R6","doi-asserted-by":"crossref","unstructured":"Karnes WE, Samloff IM, Siurala M, Kekki M, Sipponen P, Kim SW, et al. Positive serum antibody and negative tissue staining for Helicobacter pylori in subjects with atrophic body gastritis. Gastroenterology1991;101:167-174.","DOI":"10.1016\/0016-5085(91)90474-Y"},{"key":"2020022801241652300_R7","doi-asserted-by":"crossref","unstructured":"Kuipers EJ, Uyterlinde AM, Pena AS, Roosendaal R, Pals G, Nelis GF, et al. Long-term sequelae of Helicobacter pylori gastritis. Lancet1995;345:1525-1528.","DOI":"10.1016\/S0140-6736(95)91084-0"},{"key":"2020022801241652300_R8","doi-asserted-by":"crossref","unstructured":"Malfertheiner P, Me\u0301graud F, O\u2019Morain C, Hungin AP, Jones R, Axon ATR, . European Helicobacter Pylori Study Group (EHPSG)et al. Current concepts in the management of Helicobacter pylori infection: the Maastricht 2-2000 Consensus Report [Review]. Aliment Pharmacol Ther2002;16:167-180.","DOI":"10.1046\/j.1365-2036.2002.01169.x"},{"key":"2020022801241652300_R9","unstructured":"Uemura N, Mukai T, Okamoto S, Yamaguchi S, Mashiba H, Taniyama K, et al. Effect of Helicobacter pylori eradication on subsequent development of cancer after endoscopic resection of early gastric cancer. Cancer Epidemiol Biomarkers Prev1997;6:639-642."},{"key":"2020022801241652300_R10","doi-asserted-by":"crossref","unstructured":"Annibale B, Negrini R, Caruana P, Lahner E, Grossi C, Bordi C, et al. Two-thirds of atrophic body gastritis patients have evidence of Helicobacter pylori infection. Helicobacter2001;6:225-233.","DOI":"10.1046\/j.1083-4389.2001.00032.x"},{"key":"2020022801241652300_R11","doi-asserted-by":"crossref","unstructured":"Kokkola A, Rautelin H, Puolakkainen P, Sipponen P, Farkkila M, Haapiainen R, et al. Diagnosis of Helicobacter pylori infection in patients with atrophic gastritis: comparison of histology, 13C-urea breath test, and serology. Scand J Gastroenterol2000;35:138-141.","DOI":"10.1080\/003655200750024290"},{"key":"2020022801241652300_R12","doi-asserted-by":"crossref","unstructured":"Kokkola A, Rautelin H, Puolakkainen P, Sipponen P, Farkkila M, Haapiainen R, et al. Positive result by serology indicates active Helicobacter pylori infection in patients with atrophic gastritis. J Clin Microbiol1998;36:1808-1810.","DOI":"10.1128\/JCM.36.6.1808-1810.1998"},{"key":"2020022801241652300_R13","doi-asserted-by":"crossref","unstructured":"El-Omar EM, Oien K, El-Nujumi A, Gillen D, Wirz A, Dahill S, et al. Helicobacter pylori infection and chronic gastric acid hyposecretion. Gastroenterology1997;113:15-24.","DOI":"10.1016\/S0016-5085(97)70075-1"},{"key":"2020022801241652300_R14","doi-asserted-by":"crossref","unstructured":"Annibale B, Aprile MR, D\u2019Ambra G, Caruana P, Bordi C, Delle Fave G. Cure of Helicobacter pylori infection in atrophic body gastritis patients does not improve mucosal atrophy but reduces hypergastrinemia and its related effects on body ECL-cell hyperplasia. Aliment Pharmacol Ther2000;14:625-634.","DOI":"10.1046\/j.1365-2036.2000.00752.x"},{"key":"2020022801241652300_R15","doi-asserted-by":"crossref","unstructured":"Kokkola A, Sipponen P, Rautelin H, Harkonen M, Kosunen TU, Haapiainen R, et al. The effect of Helicobacter pylori eradication on the natural course of atrophic gastritis with dysplasia. Aliment Pharmacol Ther2002;16:515-520.","DOI":"10.1046\/j.1365-2036.2002.01214.x"},{"key":"2020022801241652300_R16","doi-asserted-by":"crossref","unstructured":"Faisal MA, Russell RM, Samloff IM, Holt PR. Helicobacter pylori infection and atrophic gastritis in the elderly. Gastroenterology1990;99:1543-1544.","DOI":"10.1016\/0016-5085(90)91213-P"},{"key":"2020022801241652300_R17","doi-asserted-by":"crossref","unstructured":"Liston R, Pitt MA, Banerjee AK. IgG ELISA antibodies and detection of Helicobacter pylori in elderly patients. Lancet1996;347:269-270.","DOI":"10.1016\/S0140-6736(96)90450-X"},{"key":"2020022801241652300_R18","doi-asserted-by":"crossref","unstructured":"Lahner E, Bordi C, Di Giulio E, Caruana P, D\u2019Ambra G, Milione M, et al. Role of Helicobacter pylori serology in atrophic body gastritis after eradication treatment. Aliment Pharmacol Ther2002;16:507-514.","DOI":"10.1046\/j.1365-2036.2002.01213.x"},{"key":"2020022801241652300_R19","doi-asserted-by":"crossref","unstructured":"Annibale B, Di Giulio E, Caruana P, Lahner E, Capurso G, Bordi C, et al. The long-term effects of cure of Helicobacter pylori infection on patients with atrophic body gastritis. Aliment Pharmacol Ther2002;16:1723-1731.","DOI":"10.1046\/j.1365-2036.2002.01336.x"},{"key":"2020022801241652300_R20","doi-asserted-by":"crossref","unstructured":"Satoh K, Kimura K, Taniguchi Y, Kihira K, Takimoto T, Saifuku K, et al. Biopsy sites suitable for the diagnosis of Helicobacter pylori infection and the assessment of the extent of atrophic gastritis. Am J Gastroenterol1998;93:569-573.","DOI":"10.1111\/j.1572-0241.1998.166_b.x"},{"key":"2020022801241652300_R21","doi-asserted-by":"crossref","unstructured":"Leammli UK. Cleavage of structural proteins during assembly of head of bacteriophage T4. Nature1970;227:680-683.","DOI":"10.1038\/227680a0"},{"key":"2020022801241652300_R22","doi-asserted-by":"crossref","unstructured":"Santucci A, Trabalzini L, Bovalini L, Neri P, Martelli P. Differences between predicted and observed sequences in Saccharomyces cerevisiae. Electrophoresis2000;21:37123-37171.","DOI":"10.1002\/1522-2683(200011)21:17<3717::AID-ELPS3717>3.0.CO;2-4"},{"key":"2020022801241652300_R23","doi-asserted-by":"crossref","unstructured":"Hochstrasser DF, Patchornik A, Merril CR. Development of polyacrylamide gels that improve the separation of proteins and their detection by silver staining. Anal Biochem1988;173:412-423.","DOI":"10.1016\/0003-2697(88)90208-4"},{"key":"2020022801241652300_R24","doi-asserted-by":"crossref","unstructured":"Dixon MF, Genta RM, Yardley JH, Correa P. Classification and grading of gastritis: the updated Sydney system. Am J Surg Pathol1996;20:1161-1181.","DOI":"10.1097\/00000478-199610000-00001"},{"key":"2020022801241652300_R25","doi-asserted-by":"crossref","unstructured":"Ho B, Marshall BJ. Accurate diagnosis of Helicobacter pylori. Serologic testing. Gastroenterol Clin North Am2000;29:853-862.","DOI":"10.1016\/S0889-8553(05)70152-7"},{"key":"2020022801241652300_R26","doi-asserted-by":"crossref","unstructured":"Towbin H, Stahelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A1979;76:4350-4354.","DOI":"10.1073\/pnas.76.9.4350"},{"key":"2020022801241652300_R27","doi-asserted-by":"crossref","unstructured":"Kokkola A, Kosunen TU, Puolakkainen P, Sipponen P, Harkonen M, Laxen F, et al. Spontaneous disappearance of Helicobacter pylori antibodies in patients with advanced atrophic corpus gastritis. APMIS2003;111:619-624.","DOI":"10.1034\/j.1600-0463.2003.1110604.x"},{"key":"2020022801241652300_R28","doi-asserted-by":"crossref","unstructured":"Stoicov C, Saffari R, Cai X, Hasyagar C, Houghton J. Molecular biology of gastric cancer: Helicobacter infection and gastric adenocarcinoma: bacterial and host factors responsible for altered growth signaling. Gene2004;341:1-17.","DOI":"10.1016\/j.gene.2004.07.023"},{"key":"2020022801241652300_R29","doi-asserted-by":"crossref","unstructured":"Sorberg M, Engstrand L, Strom M, Jonsson KA, Jorbeck H, Granstrom M. The diagnostic value of enzyme immunoassay and immunoblot in monitoring eradication of Helicobacter pylori. Scand J Infect Dis1997;29:147-151.","DOI":"10.3109\/00365549709035875"},{"key":"2020022801241652300_R30","doi-asserted-by":"crossref","unstructured":"Jungblut PR. Proteome analysis of bacterial pathogens. Microbes Infect2001;3:8831-8840.","DOI":"10.1016\/S1286-4579(01)01441-1"},{"key":"2020022801241652300_R31","doi-asserted-by":"crossref","unstructured":"Lepper PM, Moricke A, Vogt K, Bode G, Trautmann M. Comparison of different criteria for interpretation of immunoglobulin G immunoblotting results for diagnosis of Helicobacter pylori infection. Clin Diagn Lab Immunol2004;11:569-576.","DOI":"10.1128\/CDLI.11.3.569-576.2004"},{"key":"2020022801241652300_R32","doi-asserted-by":"crossref","unstructured":"Jungblut PR, Bumann D, Haas G, Zimny-Arndt U, Holland P, Lamer S, et al. Comparative proteome analysis of Helicobacter pylori. Mol Microbiol2000;36:710-725.","DOI":"10.1046\/j.1365-2958.2000.01896.x"},{"key":"2020022801241652300_R33","doi-asserted-by":"crossref","unstructured":"Voland P, Weeks DL, Vaira D, Prinz C, Sachs G. Specific identification of three low molecular weight membrane-associated antigens of Helicobacter pylori. Aliment Pharmacol Ther2002;16:533-544.","DOI":"10.1046\/j.1365-2036.2002.01221.x"},{"key":"2020022801241652300_R34","doi-asserted-by":"crossref","unstructured":"Genta RM. Helicobacter pylori, inflammation, mucosal damage, and apoptosis: pathogenesis and definition of gastric atrophy. Gastroenterology1997;113(Suppl 6):S51-S55.","DOI":"10.1016\/S0016-5085(97)80012-1"},{"key":"2020022801241652300_R35","doi-asserted-by":"crossref","unstructured":"Lahner E, Vaira D, Figura N, Pilozzi E, Pasquali A, Severi C, et al. Role of noninvasive tests (C-urea breath test and stool antigen test) as additional tools in diagnosis of Helicobacter pylori infection in patients with atrophic body gastritis. Helicobacter2004;9:436-442.","DOI":"10.1111\/j.1083-4389.2004.00262.x"},{"key":"2020022801241652300_R36","doi-asserted-by":"crossref","unstructured":"Kamada T, Haruma K, Hata J, Kusunoki H, Sasaki A, Ito M, et al. The long-term effect of Helicobacter pylori eradication therapy on symptoms in dyspeptic patients with fundic atrophic gastritis. Aliment Pharmacol Ther2003;18:245-252.","DOI":"10.1046\/j.1365-2036.2003.01669.x"},{"key":"2020022801241652300_R37","doi-asserted-by":"crossref","unstructured":"Cutler AF, Havstad S, Ma CK, Blaser MJ, Perez-Perez GI, Schubert TT. Accuracy of invasive and noninvasive tests to diagnose Helicobacter pylori infection. Gastroenterology1995;109:136-141.","DOI":"10.1016\/0016-5085(95)90278-3"},{"key":"2020022801241652300_R38","doi-asserted-by":"crossref","unstructured":"Crabtree JE, Shallcross TM, Heatley RV, Wyatt JI. Evaluation of a commercial ELISA for serodiagnosis of Helicobacter pylori infection. J Clin Pathol1991;44:326-328.","DOI":"10.1136\/jcp.44.4.326"},{"key":"2020022801241652300_R39","doi-asserted-by":"crossref","unstructured":"Kosunen TU, Seppala K, Sarna S, Sipponen P. Diagnostic value of decreasing IgG, IgA, and IgM antibody titres after eradication of Helicobacter pylori. Lancet1992;339:893-895.","DOI":"10.1016\/0140-6736(92)90929-W"},{"key":"2020022801241652300_R40","doi-asserted-by":"crossref","unstructured":"Ekstrom AM, Held M, Hansson LE, Engstrand L, Nyren O. Helicobacter pylori in gastric cancer established by CagA immunoblot as a marker of past infection. Gastroenterology2001;121:784-791.","DOI":"10.1053\/gast.2001.27999"}],"container-title":["Clinical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/clinchem\/article-pdf\/52\/2\/220\/32685223\/clinchem0220.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/clinchem\/article-pdf\/52\/2\/220\/32685223\/clinchem0220.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,2,28]],"date-time":"2020-02-28T06:25:10Z","timestamp":1582871110000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/clinchem\/article\/52\/2\/220\/5626777"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,2,1]]},"references-count":40,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2006,2,1]]},"published-print":{"date-parts":[[2006,2,1]]}},"URL":"https:\/\/doi.org\/10.1373\/clinchem.2005.054627","relation":{},"ISSN":["0009-9147","1530-8561"],"issn-type":[{"value":"0009-9147","type":"print"},{"value":"1530-8561","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2006,2,1]]},"published":{"date-parts":[[2006,2,1]]}}}