{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:15:10Z","timestamp":1761675310335},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2006,2,1]],"date-time":"2006-02-01T00:00:00Z","timestamp":1138752000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Background: Clinical assessment of vitamin D status often relies on measuring total circulating 25-hydroxyvitamin D3 (25OHD3), but much of each vitamin D metabolite is bound to plasma vitamin D\u2013binding protein (DBP), such that the percentage of free vitamin is very low. We hypothesized that measurement of free rather than total 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] and 25OHD3 may provide better assessment of vitamin D status. We therefore aimed to assess vitamin D status in men with idiopathic osteoporosis, in whom possible secondary causes of osteoporosis had been excluded, and to determine the extent of change in biologically active \u201cfree\u201d vitamin D caused by variation in plasma DBP concentrations.<\/jats:p>\n               <jats:p>Methods: We measured 1,25(OH)2D3 and 25OHD3 in plasma samples from 56 men with idiopathic osteoporosis [mean (SD) age, 59.6 (13.6) years; range, 21\u201386 years] and 114 male controls [62.4 (10.4) years; range, 44\u201382 years].<\/jats:p>\n               <jats:p>Results: Mean total plasma 25OHD3 in the 56 men with osteoporosis and the 114 controls was 44.7 (21) and 43.3 (17) nmol\/L, respectively; total plasma 1,25(OH)2D3 measured in randomly selected men with osteoporosis (n = 50) and controls (n = 50) was 90 (37) and 103 (39) pmol\/L, respectively. Mean plasma DBP was significantly higher (P &amp;lt;0.001) in men with osteoporosis [224 (62) mg\/L; n = 56] than in the controls [143 (34) mg\/L; n = 114], but calculated free plasma 25OHD3 and 1,25(OH)2D3 were significantly lower in the osteoporotic men than in controls [6.1 (3.1) vs 9.1 (4.4) pmol\/L (P &amp;lt;0.00001) and 77 (37) vs 142 (58) fmol\/L (P &amp;lt;0.00001), respectively].<\/jats:p>\n               <jats:p>Conclusions: Measurement of total vitamin D metabolites alone, although providing a crude assessment of vitamin D status, may not give an accurate indication of the free (biologically active) form of the vitamin. The ratio of total 25OHD3 and 1,25(OH)2D3 to plasma DBP, rather than total circulating vitamin D metabolites, may provide a more useful index of biological activity. Further studies are required to substantiate this hypothesis.<\/jats:p>","DOI":"10.1373\/clinchem.2005.059568","type":"journal-article","created":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T01:43:45Z","timestamp":1134092625000},"page":"248-254","source":"Crossref","is-referenced-by-count":47,"title":["Assessment of Vitamin D Status in Male Osteoporosis"],"prefix":"10.1093","volume":"52","author":[{"given":"Ziad H","family":"Al-oanzi","sequence":"first","affiliation":[{"name":"School of Clinical & Laboratory Sciences and School of Clinical Medical Sciences, The Medical School, University of Newcastle, Newcastle upon Tyne, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen P","family":"Tuck","sequence":"first","affiliation":[{"name":"School of Clinical & Laboratory Sciences and School of Clinical Medical Sciences, The Medical School, University of Newcastle, Newcastle upon Tyne, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas","family":"Raj","sequence":"first","affiliation":[{"name":"Departments of Rheumatology and Biochemistry, Derbyshire Royal Infirmary, Derby, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John S","family":"Harrop","sequence":"first","affiliation":[{"name":"Departments of Rheumatology and Biochemistry, Derbyshire Royal Infirmary, Derby, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory D","family":"Summers","sequence":"first","affiliation":[{"name":"Departments of Rheumatology and Biochemistry, Derbyshire Royal Infirmary, Derby, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David B","family":"Cook","sequence":"first","affiliation":[{"name":"School of Clinical & Laboratory Sciences and School of Clinical Medical Sciences, The Medical School, University of Newcastle, Newcastle upon Tyne, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger M","family":"Francis","sequence":"first","affiliation":[{"name":"School of Clinical & Laboratory Sciences and School of Clinical Medical Sciences, The Medical School, University of Newcastle, Newcastle upon Tyne, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harish K","family":"Datta","sequence":"first","affiliation":[{"name":"School of Clinical & Laboratory Sciences and School of Clinical Medical Sciences, The Medical School, University of Newcastle, Newcastle upon Tyne, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2006,2,1]]},"reference":[{"key":"2020022801241863600_R1","doi-asserted-by":"crossref","unstructured":"Cantorna MT. 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