{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:44:24Z","timestamp":1790628264341,"version":"4.1.0"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2006,2,1]],"date-time":"2006-02-01T00:00:00Z","timestamp":1138752000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Background: Detection of parapoxviruses is important in various animals as well as in humans as zoonotic infections. Reliable detection of parapoxviruses is fundamental for the exclusion of other rash-causing illnesses, for both veterinarians and medical practitioners. To date, however, no real-time PCR assay for the detection of parapoxviruses has been reported.<\/jats:p>\n               <jats:p>Methods: A minor groove binder\u2013based quantitative real-time PCR assay targeting the B2L gene of parapoxviruses was developed on the ABI Prism and the LightCycler platforms.<\/jats:p>\n               <jats:p>Results: The real-time PCR assay successfully amplified DNA fragments from a total of 41 parapoxvirus strains and isolates representing the species orf virus, bovine papular stomatitis virus, pseudocowpoxvirus, and sealpoxvirus. Probit analysis gave a limit of detection of 4.7 copies per assay (95% confidence interval, 3.7\u20136.8 copies per reaction). Scabs contain a sufficient amount of parapoxvirus DNA and can therefore be used for PCR without any DNA preparation step. No cross-reactivity to human, bovine, or sheep genomic DNA or other DNA viruses, including orthopoxviruses, molluscum contagiosum viruses, and yaba-like disease viruses, was observed.<\/jats:p>\n               <jats:p>Conclusion: The presented assay is suitable for the detection of parapoxvirus infections in clinical material of human and animal origin.<\/jats:p>","DOI":"10.1373\/clinchem.2005.060335","type":"journal-article","created":{"date-parts":[[2006,1,30]],"date-time":"2006-01-30T22:03:17Z","timestamp":1138658597000},"page":"316-319","source":"Crossref","is-referenced-by-count":70,"title":["Real-Time PCR Detection of Parapoxvirus DNA,"],"prefix":"10.1093","volume":"52","author":[{"given":"Andreas","family":"Nitsche","sequence":"first","affiliation":[{"name":"Robert Koch-Institut, Zentrum fu\u0308r Biologische Sicherheit 1, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"Bu\u0308ttner","sequence":"first","affiliation":[{"name":"Bayerisches Landesamt fu\u0308r Gesundheit und Lebensmittelsicherheit, Oberschleissheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja","family":"Wilhelm","sequence":"first","affiliation":[{"name":"Institut fu\u0308r Tierhygiene und O\u0308ffentliches Veterina\u0308rwesen, Leipzig, Germany; Bundeswehr Institute of Microbiology, Mu\u0308nchen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Pauli","sequence":"first","affiliation":[{"name":"Robert Koch-Institut, Zentrum fu\u0308r Biologische Sicherheit 1, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermann","family":"Meyer","sequence":"first","affiliation":[{"name":"Robert Koch-Institut, Zentrum fu\u0308r Biologische Sicherheit 1, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2006,2,1]]},"reference":[{"key":"2020022801220285100_R1","unstructured":"Fields BN, Knipe DM, Howley PM. 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