{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:08:20Z","timestamp":1791295700029,"version":"4.1.0"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2006,9,1]],"date-time":"2006-09-01T00:00:00Z","timestamp":1157068800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Background: Measurements of aldosterone have become more common since the recognition that primary aldosteronism is a more frequent cause of hypertension than previously believed. Our aim was to compare concentrations reported by 4 assays for samples obtained after saline infusion during dynamic testing.<\/jats:p>\n               <jats:p>Methods: We tested 104 participants (27 with primary aldosteronism, 30 with essential hypertension, and 47 healthy controls) with the intravenous saline infusion test (2.0 L isotonic saline over 4 h), with repetitive sampling. In all blood samples, aldosterone concentration was measured by an in-house RIA after extraction and chromatography, by 2 commercially available RIAs without extraction (Aldosterone Maia, Adaltis; Active Aldosterone, Diagnostics Systems Laboratories) and by an automated CLIA (Advantage, Nichols Institute Diagnostics).<\/jats:p>\n               <jats:p>Results: Correlation coefficients for results of pairs of assays ranged from 0.74 to 0.98. Agreement between commercial assays and in-house RIA was best at the low to intermediate concentrations after saline infusion. Mean (SD) Adaltis and DSL RIA results were 2- to 3-times higher [healthy participants: 78 (25) ng\/L and 56 (18) ng\/L, respectively] than those obtained by Nichols CLIA [17 (8) ng\/L] and in-house RIA [23 (18) ng\/L]. Aldosterone concentrations measured by the Nichols CLIA were below the limit of detection (limit of the blank) in 27 of 47 healthy participants.<\/jats:p>\n               <jats:p>Conclusions: Aldosterone concentrations reported by the Adaltis and DSL nonextraction RIAs were consistently higher than those produced by the Nichols CLIA and the in-house RIA. The convenient Nichols CLIA showed better agreement with the in-house RIA, but the concentrations in healthy participants were frequently undetectable by this method. Uncritical application of cutoff values from the literature must be avoided.<\/jats:p>","DOI":"10.1373\/clinchem.2006.068502","type":"journal-article","created":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T21:04:43Z","timestamp":1153429483000},"page":"1749-1755","source":"Crossref","is-referenced-by-count":110,"title":["Automated Chemiluminescence-Immunoassay for Aldosterone during Dynamic Testing: Comparison to Radioimmunoassays with and without Extraction Steps"],"prefix":"10.1093","volume":"52","author":[{"given":"Caroline","family":"Schirpenbach","sequence":"first","affiliation":[{"name":"Ludwig-Maximilians-University, Medizinische Klinik Innenstadt, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lysann","family":"Seiler","sequence":"first","affiliation":[{"name":"Centre Hospitalier Universitaire Vaudois, De\u0301partement de me\u0301decine interne Lausanne, Lausanne, Switzerland"},{"name":"Albert-Ludwigs-University, Medical Department 2, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiane","family":"Maser-Gluth","sequence":"first","affiliation":[{"name":"Ruprecht-Karls-University, Institute of Pharmacology, Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix","family":"Beuschlein","sequence":"first","affiliation":[{"name":"Albert-Ludwigs-University, Medical Department 2, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Reincke","sequence":"first","affiliation":[{"name":"Ludwig-Maximilians-University, Medizinische Klinik Innenstadt, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Bidlingmaier","sequence":"first","affiliation":[{"name":"Ludwig-Maximilians-University, Medizinische Klinik Innenstadt, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2006,9,1]]},"reference":[{"key":"2020022801253672900_R1","doi-asserted-by":"crossref","unstructured":"McKenna T, Sequeira S, Heffernan A, Chambers J, Cunningham S. 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