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These studies require the development and implementation of reliable and large-scale genotyping methods. In this report, we introduce an efficient technique based on PCR\u2013restriction fragment length sequence variation technique for the analysis of the \u2212360CG and \u2212201CT single-nucleotide sequence variations in the deoxycytidine kinase gene.<\/jats:p><jats:p>Methods: A multicapillary gel electrophoresis instrument was used for the size determination of the generated DNA fragments. A healthy Hungarian population of 100 individuals was investigated to determine allele and genotype frequencies for the 2 sequence variations of interest.<\/jats:p><jats:p>Results: We found that the occurrence of the minor allele is rather low, i.e., the frequency of both the \u2212360G and \u2212201T variants is 1%.<\/jats:p><jats:p>Conclusions: Our technique can readily facilitate the analysis of these important sequence variations in other ethnic groups to clarify the role of these sequence variations in conjunction with arabinosylcytosine treatment in acute myeloid leukemia.<\/jats:p>","DOI":"10.1373\/clinchem.2006.071159","type":"journal-article","created":{"date-parts":[[2006,7,21]],"date-time":"2006-07-21T01:04:43Z","timestamp":1153443883000},"page":"1756-1762","source":"Crossref","is-referenced-by-count":7,"title":["Multicapillary Electrophoresis Analysis of Single-Nucleotide Sequence Variations in the Deoxycytidine Kinase Gene"],"prefix":"10.1093","volume":"52","author":[{"given":"Eszter","family":"Szantai","sequence":"first","affiliation":[{"name":"Horva\u0301th Laboratory of Bioseparation Sciences and Institute of Analytical Chemistry and Radiochemistry, Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zsolt","family":"Ronai","sequence":"first","affiliation":[{"name":"Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Sasvari-Szekely","sequence":"first","affiliation":[{"name":"Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gu\u0308nther","family":"Bonn","sequence":"first","affiliation":[{"name":"Horva\u0301th Laboratory of Bioseparation Sciences and Institute of Analytical Chemistry and Radiochemistry, Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andra\u0301s","family":"Guttman","sequence":"first","affiliation":[{"name":"Horva\u0301th Laboratory of Bioseparation Sciences and Institute of Analytical Chemistry and Radiochemistry, Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2006,9,1]]},"reference":[{"key":"2020022801251413200_R1","doi-asserted-by":"crossref","unstructured":"Irizarry KJ, Galbraith SJ. 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