{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T08:48:19Z","timestamp":1787042899144,"version":"build-2736575974"},"reference-count":11,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2007,8,1]],"date-time":"2007-08-01T00:00:00Z","timestamp":1185926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["K24 DK074457"],"award-info":[{"award-number":["K24 DK074457"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["32333"],"award-info":[{"award-number":["32333"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP-7643"],"award-info":[{"award-number":["MOP-7643"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Background: A new parathyroid hormone (PTH) species, the N-terminal PTH form (N-PTH), is distinct from intact human PTH of 84 amino acid residues [hPTH(1\u201384)] and is recognized in a 3rd-generation assay of \u201cwhole\u201d PTH (wPTH; the 1\u20132 epitope) but not in a 2nd-generation assay of \u201ctotal\u201d PTH (tPTH; the 12\u201318 epitope). N-PTH usually represents &amp;lt;15% of wPTH but can be overproduced in severe primary hyperparathyroidism (PHPT) and secondary hyperparathyroidism. We investigated whether N-PTH is also overproduced in parathyroid cancer and whether N-PTH concentration is influenced by calcimimetic therapy.<\/jats:p>\n                  <jats:p>Methods: We studied 8 patients with parathyroid carcinoma before and at week 16 of cinacalcet therapy, 6 patients with PHPT, and 6 control individuals. We fractionated sera with HPLC and analyzed fractions with the 2 assays to quantify hPTH(1\u201384), N-PTH, and non-(1\u201384) PTH fragments.<\/jats:p>\n                  <jats:p>Results: Half of parathyroid carcinoma patients had an increased wPTH:tPTH ratio [mean (SD), 1.35 (0.29)]; the others had a typical ratio [0.72 (0.12)]. HPLC fractionation of sera from 2 high-ratio patients confirmed N-PTH overproduction [65% (12%) of wPTH]. The N-PTH fraction was &amp;lt;15% of wPTH in PHPT and healthy individuals. Calcimimetic therapy appreciably reduced calcium concentrations in parathyroid carcinoma patients but had little influence on PTH concentration, the wPTH:tPTH ratio, or the PTH HPLC profile.<\/jats:p>\n                  <jats:p>Conclusion: N-PTH is overproduced in some parathyroid cancer patients, but calcimimetic therapy does not influence its production. The clinical implications of this finding in parathyroid carcinoma await additional studies with an emphasis on N-PTH\u2019s biological activity and with larger numbers of patients.<\/jats:p>","DOI":"10.1373\/clinchem.2007.085506","type":"journal-article","created":{"date-parts":[[2007,6,28]],"date-time":"2007-06-28T20:40:07Z","timestamp":1183063207000},"page":"1470-1476","source":"Crossref","is-referenced-by-count":79,"title":["An N-Terminal Molecular Form of Parathyroid Hormone (PTH) Distinct from hPTH(1\u201384) Is Overproduced in Parathyroid Carcinoma"],"prefix":"10.1093","volume":"53","author":[{"given":"Mishaela R","family":"Rubin","sequence":"first","affiliation":[{"name":"College of Physicians and Surgeons, Columbia University, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shonni J","family":"Silverberg","sequence":"first","affiliation":[{"name":"College of Physicians and Surgeons, Columbia University, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"D\u2019Amour","sequence":"first","affiliation":[{"name":"Centre de Recherche du Centre Hospitalier de l\u2019Universite\u0301 de Montre\u0301al, Ho\u0302pital Saint-Luc, Montreal, Que\u0301bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Hugues","family":"Brossard","sequence":"first","affiliation":[{"name":"Centre de Recherche du Centre Hospitalier de l\u2019Universite\u0301 de Montre\u0301al, Ho\u0302pital Saint-Luc, Montreal, Que\u0301bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Rousseau","sequence":"first","affiliation":[{"name":"Centre de Recherche du Centre Hospitalier de l\u2019Universite\u0301 de Montre\u0301al, Ho\u0302pital Saint-Luc, Montreal, Que\u0301bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"James","family":"Sliney","sequence":"first","affiliation":[{"name":"College of Physicians and Surgeons, Columbia University, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Cantor","sequence":"first","affiliation":[{"name":"Scantibodies Laboratory Inc., Santee, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P","family":"Bilezikian","sequence":"first","affiliation":[{"name":"College of Physicians and Surgeons, Columbia University, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,8,1]]},"reference":[{"key":"2020022802453741500_R1","doi-asserted-by":"crossref","unstructured":"Gao P, Scheibel S, D\u2019Amour P, John MR, Rao SD, Schmidt-Gayk H, et al. Development of a novel immunoradiometric assay exclusively for biologically active whole parathyroid hormone 1\u201384: implications for improvement of accurate assessment of parathyroid function. J Bone Miner Res2001;16:605-614.","DOI":"10.1359\/jbmr.2001.16.4.605"},{"key":"2020022802453741500_R2","doi-asserted-by":"crossref","unstructured":"D\u2019Amour P, Brossard JH, Rousseau L, Roy L, Gao P, Cantor T. Amino-terminal form of parathyroid hormone (PTH) with immunologic similarities to hPTH(1\u201384) is overproduced in primary and secondary hyperparathyroidism. Clin Chem2003;49:2037-2044.","DOI":"10.1373\/clinchem.2003.021592"},{"key":"2020022802453741500_R3","doi-asserted-by":"crossref","unstructured":"D\u2019Amour P, Brossard JH, Rakel A, Rousseau L, Albert C, Cantor T. Evidence that the amino-terminal composition of non-(1\u201384) parathyroid hormone fragments starts before position 19. Clin Chem2005;51:169-176.","DOI":"10.1373\/clinchem.2004.040485"},{"key":"2020022802453741500_R4","doi-asserted-by":"crossref","unstructured":"Rakel A, Brossard JH, Patenaude JV, Albert C, Nassif E, Cantor T, et al. Overproduction of an amino-terminal form of PTH distinct from human PTH(1\u201384) in a case of severe primary hyperparathyroidism: influence of medical treatment and surgery. Clin Endocrinol2005;62:721-727.","DOI":"10.1111\/j.1365-2265.2005.02286.x"},{"key":"2020022802453741500_R5","doi-asserted-by":"crossref","unstructured":"Arakawa T, D\u2019Amour P, Rousseau L, Brossard J, Sakai M, Kasumoto H, et al. Overproduction and secretion of a novel amino-terminal form of parathyroid hormone from a severe type of parathyroid hyperplasia in uremia. Clin J Am Soc Nephrol2006;1:525-531.","DOI":"10.2215\/CJN.01391005"},{"key":"2020022802453741500_R6","doi-asserted-by":"crossref","unstructured":"John MR, Goodman WG, Gao P, Cantor TL, Salusky IB, Juppner H. 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Structure of non-(1\u201384) PTH fragments secreted by parathyroid glands in primary and secondary hyperparathyroidism. Kidney Int2005;68:998-1007.","DOI":"10.1111\/j.1523-1755.2005.00493.x"},{"key":"2020022802453741500_R10","doi-asserted-by":"crossref","unstructured":"Rabbani SA, Kremer R, Bennett HP, Goltzman D. Phosphorylation of parathyroid hormone by human and bovine parathyroid glands. J Biol Chem1984;259:2949-2955.","DOI":"10.1016\/S0021-9258(17)43241-8"},{"key":"2020022802453741500_R11","doi-asserted-by":"crossref","unstructured":"Tanaka M, Itoh K, Matsushita K, Matsushita K, Fujii H, Fukagawa M. Normalization of reversed bio-intact-PTH(1\u201384)\/intact-PTH ratio after parathyroidectomy in a patient with severe secondary hyperparathyroidism. 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