{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T07:01:07Z","timestamp":1790924467446,"version":"4.1.0"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T00:00:00Z","timestamp":1204329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Background: Patients with inflammatory bowel disease (IBD) carry autoantibodies such as perinuclear antineutrophil cytoplasmic antibodies (pANCA). \u03b1-Enolase has been proposed as a target antigen in IBD. We evaluated the prevalence and diagnostic value of anti\u2013\u03b1-enolase antibodies in IBD and related disorders.<\/jats:p>\n                  <jats:p>Methods: We used a classic proteomic approach with extracts from granulocytes and pANCA-positive ulcerative colitis (UC) sera to confirm \u03b1-enolase as a target antigen. By means of Western blot analysis, we screened a cohort of 525 subjects for the presence of anti\u2013\u03b1-enolase antibodies. We performed GeneArray experiments on RNA extracted from colonic mucosal biopsies from 35 IBD and 6 control patients.<\/jats:p>\n                  <jats:p>Results: We detected anti\u2013\u03b1-enolase antibodies 49.0% of patients with UC, 50.0% of patients with Crohn\u2019s disease, 30.5% of patients with primary sclerosing cholangitis, 37.8% of patients with autoimmune hepatitis, 34.0% of patients with ANCA-positive vasculitis, 31.0% of non-IBD gastrointestinal controls, and 8.5% of healthy controls. Gene array experiments showed a significant upregulation of \u03b1-enolase mRNA in colonic mucosal biopsies from patients with IBD, but not from controls. There was no association between the presence of pANCA and anti\u2013\u03b1-enolase antibodies. Preabsorption with \u03b1-enolase did not eliminate the pANCA pattern on indirect immunofluorescence.<\/jats:p>\n                  <jats:p>Conclusions: Anti\u2013\u03b1-enolase antibodies are present in a substantial proportion of patients with IBD, patients with various inflammatory\/autoimmune disorders, and non-IBD gastrointestinal controls. Therefore, anti\u2013\u03b1-enolase antibodies are of limited diagnostic value for the diagnosis of IBD.<\/jats:p>","DOI":"10.1373\/clinchem.2007.098368","type":"journal-article","created":{"date-parts":[[2008,1,24]],"date-time":"2008-01-24T20:54:16Z","timestamp":1201208056000},"page":"534-541","source":"Crossref","is-referenced-by-count":42,"title":["Anti\u2013\u03b1-enolase Antibodies in Patients with Inflammatory Bowel Disease"],"prefix":"10.1093","volume":"54","author":[{"given":"Nathalie","family":"Vermeulen","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, University Hospital Leuven"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Arijs","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, University Hospital Leuven"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofie","family":"Joossens","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, University 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