{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T04:04:20Z","timestamp":1790395460564,"version":"4.1.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T00:00:00Z","timestamp":1228089600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Background: Measurement of methylmalonic acid (MMA) in serum or plasma is useful for diagnosing cobalamin deficiency. We developed a method for quantifying MMA in plasma based on hydrophilic interaction liquid chromatography (HILIC) and single-stage negative electrospray ionization (ESI) mass spectrometry.<\/jats:p>\n               <jats:p>Methods: We deproteinized plasma samples (200 \u03bcL) with 800 \u03bcL acidified acetonitrile containing 0.17 \u03bcmol\/L deuterated MMA (D3-MMA) internal standard, centrifuged the samples, and injected 4 \u03bcL of the supernatant into the LC-MS instrument. Separation was achieved within 3 min on a Merck SeQuant ZIC\u00ae-HILIC column with a mobile phase consisting of 4 volumes acetonitrile plus 1 volume 100 mmol\/L ammonium acetate buffer, pH 4.5, at a flow rate of 400 \u03bcL\/min. Subsequent column washing and reconditioning contributed to a total run time of 10 min. MMA and D3-MMA were quantified by single-ion monitoring (m\/z 117.2 and 120.2, respectively) in negative ESI mode at a drying-gas flow rate of 10 L\/min, 300 \u00b0C, and a capillary voltage of 3.0 kV.<\/jats:p>\n               <jats:p>Results: The estimated limits of MMA quantification and detection were 0.09 \u03bcmol\/L and 0.03 \u03bcmol\/L, respectively, in plasma. The assay was linear to 200 \u03bcmol\/L. Interassay and intraassay CVs were \u22645% at all tested concentrations. Recoveries were 90%\u201393%.<\/jats:p>\n               <jats:p>Conclusions: This robust assay allows analysis of MMA in human plasma without derivatization. Sample preparation is simple and suitable for automation.<\/jats:p>","DOI":"10.1373\/clinchem.2007.101253","type":"journal-article","created":{"date-parts":[[2008,10,10]],"date-time":"2008-10-10T01:53:55Z","timestamp":1223603635000},"page":"2028-2035","source":"Crossref","is-referenced-by-count":49,"title":["Quantification of Methylmalonic Acid in Human Plasma with Hydrophilic Interaction Liquid Chromatography Separation and Mass Spectrometric Detection"],"prefix":"10.1093","volume":"54","author":[{"given":"Hans-A\u030ake","family":"Lakso","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacology, University of Umea\u030a, Umea\u030a, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrik","family":"Appelblad","sequence":"first","affiliation":[{"name":"SeQuant AB, Umea\u030a, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u0308rn","family":"Schneede","sequence":"first","affiliation":[{"name":"Department of 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