{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T21:27:45Z","timestamp":1788038865174,"version":"build-2784847793"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T00:00:00Z","timestamp":1220227200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Background: The pyridinium cross-links pyridinoline (PYD) and deoxypyridinoline (DPD) are established markers of bone resorption. We evaluated the analytical and clinical performance of a commercially available PYD HPLC assay and established reference intervals in children and adults.<\/jats:p><jats:p>Methods: We used a commercially available reagent set (Chromsystems Instruments &amp; Chemicals) to measure PYD and DPD in 319 healthy controls (156 premenopausal women, 80 healthy men, and 83 healthy children age 1 month to 14 years) and 397 patients with metabolic bone diseases (postmenopausal osteoporosis, n = 175; male osteoporosis, n = 176; hyperparathyroidism, n = 17; hyperthyroidism, n = 19; Paget disease, n = 10).<\/jats:p><jats:p>Results: The mean intraassay and interassay CVs were &amp;lt;6% and &amp;lt;8% for both PYD and DPD, respectively. The reference interval was constant for premenopausal women in the age group 20\u201349 years. In men, cross-link values peaked at 20\u201329 years and decreased thereafter. Women with postmenopausal osteoporosis had significantly higher PYD (51%) and DPD (58%) values compared to premenopausal women. Similar results were found in osteoporotic men. In children the highest values were found in the first weeks and months after birth, followed by a decrease of 50%\u201360% at age 11\u201314 years. In metabolic bone diseases cross-link concentrations were significantly increased. The DPD:PYD ratio (mean value approximately 0.2) was remarkably constant in all populations evaluated.<\/jats:p><jats:p>Conclusions: The automated HPLC assay is a precise and convenient method for PYD and DPD measurement. We established reference intervals for adult women and men and for children up to 14 years old. The cross-link concentrations we determined by use of this HPLC method confirm its clinical value in enabling identification of increased bone resorption in patients with metabolic bone diseases.<\/jats:p>","DOI":"10.1373\/clinchem.2008.105262","type":"journal-article","created":{"date-parts":[[2008,7,25]],"date-time":"2008-07-25T01:54:45Z","timestamp":1216950885000},"page":"1546-1553","source":"Crossref","is-referenced-by-count":25,"title":["Automated HPLC Assay for Urinary Collagen Cross-links: Effect of Age, Menopause, and Metabolic Bone Diseases"],"prefix":"10.1093","volume":"54","author":[{"given":"Marius E","family":"Kraenzlin","sequence":"first","affiliation":[{"name":"Division of Endocrinology, Diabetes, and Clinical Nutrition, University Hospital, CH-4031 Basel, Switzerland"},{"name":"Endocrine Practice and Laboratory, Missionsstrasse 24, CH-4055 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude 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