{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:03:53Z","timestamp":1791295433894,"version":"4.1.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2009,6,1]],"date-time":"2009-06-01T00:00:00Z","timestamp":1243814400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Background: Reliable measurement of aldosterone with less interlaboratory variation than RIA would help standardize testing for primary aldosteronism. We set out to validate a high-performance liquid chromatography\u2013tandem mass spectrometry (HPLC-MS\/MS) method for aldosterone in human plasma.<\/jats:p>\n               <jats:p>Methods: We prepared samples (EDTA plasma, lithium heparin plasma, and serum from separator and plain clot tubes) and measured aldosterone using online HPLC-MS\/MS with d7-aldosterone as internal standard. We also analyzed EDTA plasma samples by immunoassay. We established a reference range for HPLC-MS\/MS aldosterone by analyzing blood collected midmorning from 97 normotensive seated subjects.<\/jats:p>\n               <jats:p>Results: The linear range was 69.4\u20135548.0 pmol\/L (2.5\u2013200 ng\/dL) (r2 &amp;gt; 0.994, n = 14). Inter- and intraday analytical recovery and imprecision for quality control samples of 166.4, 1109.6, and 4161.0 pmol\/L (6.0, 40.0, and 150.0 ng\/dL) were 92.2%\u2013102.0% and &amp;lt;6.3%, respectively (n = 5). The lower limit of quantification was 69.4 pmol\/L (2.5 ng\/dL), with inter- and intraday analytical recovery and imprecision of 91.4%\u201394.5% and &amp;lt;9.5% (n = 5). No interferences were observed in plasma from Addison\u2019s disease patients (n = 5). Comparison of collection tubes, using EDTA as the reference, revealed similar aldosterone results. Comparison of HPLC-MS\/MS with immunoassay gave an acceptable mean bias (0.83%) but wide range (\u221244.8% to 39.7%) of differences. HPLC-MS\/MS aldosterone concentrations in normotensive subjects ranged from &amp;lt;69.4 to 635.2 pmol\/L (&amp;lt;2.5 to 22.9 ng\/dL).<\/jats:p>\n               <jats:p>Conclusions: This first reported aldosterone method using online HPLC-MS\/MS is precise across the clinically relevant range, not influenced by collection tube type, and offers semiautomated sample preparation and high throughput.<\/jats:p>","DOI":"10.1373\/clinchem.2008.116004","type":"journal-article","created":{"date-parts":[[2009,4,10]],"date-time":"2009-04-10T02:02:06Z","timestamp":1239328926000},"page":"1155-1162","source":"Crossref","is-referenced-by-count":102,"title":["Measurement of Aldosterone in Human Plasma by Semiautomated HPLC\u2013Tandem Mass Spectrometry"],"prefix":"10.1093","volume":"55","author":[{"given":"Paul J","family":"Taylor","sequence":"first","affiliation":[{"name":"Endocrine Hypertension Research Centre, University of Queensland, Greenslopes and Princess Alexandra Hospitals, Brisbane, 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