{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T03:28:50Z","timestamp":1793071730785,"version":"4.5.0"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Background: Increased concentrations of plasma total homocysteine (tHcy) have been associated with age-related diseases, including dementia, stroke, and Parkinson disease (PD). Methylation status might link Hcy metabolism to neurodegenerative proteins in patients with PD.<\/jats:p><jats:p>Methods: We tested blood samples from 87 patients with PD (median age 68 years; 35 men) for tHcy, methylmalonic acid (MMA), vitamin B12, vitamin B6, folate, S-adenosyl methionine (SAM), S-adenosyl homocysteine (SAH), and amyloid-\u03b2(1\u201342). We collected citrate blood from a subset of 45 patients to prepare platelet-rich plasma, and we used washed platelets to prepare cell extracts for amyloid precursor protein (APP) and \u03b1-synuclein assays. We used brain parenchyma sonography to estimate the substantia nigra echogenic area in a subset of 59 patients.<\/jats:p><jats:p>Results: Serum concentrations of tHcy were increased in PD patients (median 14.8 \u03bcmol\/L). tHcy (\u03b2 coefficient = \u22120.276) and serum creatinine (\u03b2 = \u22120.422) were significant predictors of the ratio of SAM\/SAH in plasma (P &amp;lt; 0.01). The plasma SAM\/SAH ratio was a significant determinant for DemTect scores (\u03b2 = 0.612, P = 0.004). Significant negative correlations were found between concentrations of SAH in plasma and platelet APP and between SAM and platelet \u03b1-synuclein. A larger echogenic area of the substantia nigra was related to higher serum concentrations of MMA (P = 0.016).<\/jats:p><jats:p>Conclusions: Markers of neurodegeneration (APP, \u03b1-synuclein) are related to markers of methylation (SAM, SAH) in patients with PD. Better cognitive function was related to higher methylation potential (SAM\/SAH ratio).<\/jats:p>","DOI":"10.1373\/clinchem.2009.125021","type":"journal-article","created":{"date-parts":[[2009,8,14]],"date-time":"2009-08-14T00:40:53Z","timestamp":1250210453000},"page":"1852-1860","source":"Crossref","is-referenced-by-count":83,"title":["Methylation Status and Neurodegenerative Markers in Parkinson Disease"],"prefix":"10.1093","volume":"55","author":[{"given":"Rima","family":"Obeid","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry and Laboratory Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achim","family":"Schadt","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry and Laboratory Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Dillmann","sequence":"first","affiliation":[{"name":"Department of Neurology, University Hospital of Saarland, Homburg, 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