{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:35:01Z","timestamp":1770824101099,"version":"3.50.1"},"reference-count":12,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100004876","name":"Sheffield Hospitals Charity","doi-asserted-by":"publisher","award":["7877"],"award-info":[{"award-number":["7877"]}],"id":[{"id":"10.13039\/501100004876","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Background: Some patients with celiac disease (CD) may be seronegative with the commonly used test for IgA anti\u2013tissue transglutaminase (anti-tTG) antibodies. Our aim was to explore whether newer assays incorporating synthetic deamidated gliadin-related peptides (DGPs) or other TG isoenzymes as antigen are useful for detecting gluten sensitivity in IgA anti-tTG\u2013seronegative patients.<\/jats:p>\n               <jats:p>Methods: We assayed serum samples obtained at diagnosis from (a) anti-tTG\u2013seronegative patients with a CD-like enteropathy (n = 12), (b) skin biopsy\u2013proven dermatitis herpetiformis (DH) patients (n = 26), and (c) IgA anti-tTG\u2013positive CD patients (n = 26). All patients had typical total IgA concentrations. All patients underwent intestinal biopsy and serum testing for (a) detection of IgA and IgG isotypes of both anti-DGP and anti-tTG in a single assay (tTG\/DGP Screen; INOVA Diagnostics), (b) simultaneous detection of both IgA and IgG anti-DGP antibody isotypes (DGP Dual; INOVA Diagnostics), and (c) detection of antibodies to transglutaminase 3 (TG3) or transglutaminase 6 (TG6).<\/jats:p>\n               <jats:p>Results: All anti-tTG\u2013seropositive patients also tested positive in anti-DGP assays. Overall, tTG\/DGP Screen detected 6 (31.6%) of the 19 anti-tTG seronegatives, and anti-DGP Dual produced positive results in 5 (26.3%) of these cases. Whereas both assays detected 2 anti-tTG\u2013negative DH patients with partial villous atrophy, they were positive in only 2 of the 5 cases with no histologically discernible mucosal damage. Testing for antibodies to TG3 and TG6 identified 7 (36.8%) of the 19 anti-tTG\u2013negative patients, 5 of which were also positive for anti-DGP.<\/jats:p>\n               <jats:p>Conclusions: Detection of anti-DGP with tTG\/DGP Screen or anti-DGP Dual, or detection of antibodies to other TG isoenzymes, enhances the sensitivity for detecting gluten sensitivity among non\u2013IgA- deficient, anti-tTG\u2013seronegative patients with CD-like enteropathy.<\/jats:p>","DOI":"10.1373\/clinchem.2009.129668","type":"journal-article","created":{"date-parts":[[2009,12,18]],"date-time":"2009-12-18T21:10:43Z","timestamp":1261170643000},"page":"661-665","source":"Crossref","is-referenced-by-count":36,"title":["New Serology Assays Can Detect Gluten Sensitivity among Enteropathy Patients Seronegative for Anti\u2013Tissue Transglutaminase"],"prefix":"10.1093","volume":"56","author":[{"given":"Emilia","family":"Sugai","sequence":"first","affiliation":[{"name":"Small Bowel Section, Department of Medicine, C. Bonorino Udaondo Gastroenterology Hospital, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui Jer","family":"Hwang","sequence":"first","affiliation":[{"name":"Small Bowel Section, Department of Medicine, C. Bonorino Udaondo Gastroenterology Hospital, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Horacio","family":"Va\u0301zquez","sequence":"first","affiliation":[{"name":"Small Bowel Section, Department of Medicine, C. Bonorino Udaondo Gastroenterology Hospital, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgardo","family":"Smecuol","sequence":"first","affiliation":[{"name":"Small Bowel Section, Department of Medicine, C. Bonorino Udaondo Gastroenterology Hospital, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"Niveloni","sequence":"first","affiliation":[{"name":"Small Bowel Section, Department of Medicine, C. Bonorino Udaondo Gastroenterology Hospital, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Mazure","sequence":"first","affiliation":[{"name":"Small Bowel Section, Department of Medicine, C. Bonorino Udaondo Gastroenterology Hospital, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo","family":"Maurin\u0303o","sequence":"first","affiliation":[{"name":"Small Bowel Section, Department of Medicine, C. 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