{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T23:22:31Z","timestamp":1773271351679,"version":"3.50.1"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["CA80117"],"award-info":[{"award-number":["CA80117"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100002608","name":"American Thyroid Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100002608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Background: Molecular testing of thyroid malignancies, in combination with cytologic and histologic examination, is becoming increasingly attractive as a tool for refining traditional morphologic diagnosis. The molecular changes associated with follicular thyroid carcinoma (FTC) are point mutations in RAS oncogenes or the presence of PAX8\/PPARG (paired box 8\/peroxisome proliferator-activated receptor gamma) rearrangement.<\/jats:p>\n               <jats:p>Methods: We developed and validated a clinical assay for the detection of PAX8\/PPARG rearrangements that uses a 4-color reverse-transcription PCR (RT-PCR) assay and high-resolution fragment analysis.<\/jats:p>\n               <jats:p>Results: The RT-PCR assay is applicable for detecting the various described fusion transcripts of PAX8\/PPARG in formalin-fixed, paraffin-embedded thyroid tissue and in fine-needle aspirate biopsy washes from thyroid nodules. The analytical sensitivity of the assay is 1 abnormal cell in a background of 100\u201310 000 translocation-negative cells. A comparison of the RT-PCR assay with dual-fusion fluorescence in situ hybridization showed an overall concordance of 95%. With this assay, we obtained a prevalence for the PAX8\/PPARG rearrangement in FTC of 62% (13 of 21 cases), compared with a 5% prevalence (3 of 55) for other follicular cell\u2013derived neoplasms.<\/jats:p>\n               <jats:p>Conclusions: The introduction of this assay into clinical practice could provide useful information for the diagnosis and possibly for the prognosis and treatment of thyroid cancer in the future.<\/jats:p>","DOI":"10.1373\/clinchem.2009.134015","type":"journal-article","created":{"date-parts":[[2010,1,7]],"date-time":"2010-01-07T22:42:55Z","timestamp":1262904175000},"page":"391-398","source":"Crossref","is-referenced-by-count":25,"title":["Evaluation of the PAX8\/PPARG Translocation in Follicular Thyroid Cancer with a 4-Color Reverse-Transcription PCR Assay and Automated High-Resolution Fragment Analysis"],"prefix":"10.1093","volume":"56","author":[{"given":"Alicia","family":"Algeciras-Schimnich","sequence":"first","affiliation":[{"name":"Departments of 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