{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:29:25Z","timestamp":1790666965319,"version":"4.1.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T00:00:00Z","timestamp":1285891200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100002429","name":"Amgen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100002429","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004329","name":"Genzyme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004337","name":"Roche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004337","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,10,1]]},"abstract":"<jats:sec>\n                  <jats:title>BACKGROUND<\/jats:title>\n                  <jats:p>Hepcidin is an iron-regulatory peptide hormone that consists of 3 isoforms: bioactive hepcidin-25, and inactive hepcidin-22 and hepcidin-20. Hepcidin is instrumental in the diagnosis and monitoring of iron metabolism disorders, but reliable methods for its quantification in serum are sparse, as is knowledge of their relative analytical strengths and clinical utility.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>METHODS<\/jats:title>\n                  <jats:p>We developed a competitive (c)-ELISA and an immunocapture TOF mass-spectrometry (IC-TOF-MS) assay. Exploiting these 2 methods and our previously described weak cation exchange (WCX)-TOF-MS assay, we measured serum hepcidin concentrations in 186 patients with various disorders of iron metabolism and in 23 healthy controls.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>RESULTS<\/jats:title>\n                  <jats:p>We found that (a) the relative differences in median hepcidin concentrations in various diseases to be similar, although the absolute concentrations measured with c-ELISA and WCX-TOF-MS differed; (b) hepcidin isoforms contributed to differences in hepcidin concentrations between methods, which were most prominent in patients with chronic kidney disease; and (c) hepcidin concentrations measured by both the c-ELISA and IC-TOF-MS correlated with ferritin concentrations &amp;lt;60 \u03bcg\/L, and were suitable for distinguishing between iron deficiency anemia (IDA) and the combination of IDA and anemia of chronic disease.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>CONCLUSIONS<\/jats:title>\n                  <jats:p>c-ELISA is the method of choice for the large-scale quantification of serum hepcidin concentrations, because of its low limit of detection, low cost, and high-throughput. Because of its specificity for bioactive hepcidin-25, WCX-TOF-MS can be regarded as a valuable special-purpose assay for disorders with variable concentrations of hepcidin isoforms, such as chronic kidney disease.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1373\/clinchem.2010.149187","type":"journal-article","created":{"date-parts":[[2010,8,25]],"date-time":"2010-08-25T22:25:06Z","timestamp":1282775106000},"page":"1570-1579","source":"Crossref","is-referenced-by-count":162,"title":["Immunochemical and Mass-Spectrometry\u2013Based Serum Hepcidin Assays for Iron Metabolism Disorders"],"prefix":"10.1093","volume":"56","author":[{"given":"Joyce JC","family":"Kroot","sequence":"first","affiliation":[{"name":"Laboratory of Genetic, Endocrine and Metabolic Diseases, Department of Laboratory Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coby MM","family":"Laarakkers","sequence":"first","affiliation":[{"name":"Laboratory of Genetic, Endocrine and Metabolic Diseases, Department of Laboratory Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anneke J","family":"Geurts-Moespot","sequence":"first","affiliation":[{"name":"Laboratory of Genetic, Endocrine and Metabolic Diseases, Department of Laboratory Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola\u00ef","family":"Grebenchtchikov","sequence":"first","affiliation":[{"name":"Laboratory of Genetic, Endocrine and Metabolic Diseases, Department of Laboratory Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Pickkers","sequence":"first","affiliation":[{"name":"Department of Intensive Care Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annelies E","family":"van Ede","sequence":"first","affiliation":[{"name":"Department of Rheumatology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilde PE","family":"Peters","sequence":"first","affiliation":[{"name":"Department of Nephrology, Radboud University Nijmegen Medical Centre, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edm\u00e9e","family":"van Dongen-Lases","sequence":"first","affiliation":[{"name":"Laboratory of Genetic, Endocrine and Metabolic Diseases, Department of Laboratory Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack FM","family":"Wetzels","sequence":"first","affiliation":[{"name":"Department of Nephrology, Radboud University Nijmegen Medical Centre, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred CGJ","family":"Sweep","sequence":"first","affiliation":[{"name":"Laboratory of Genetic, Endocrine and Metabolic Diseases, Department of Laboratory Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harold","family":"Tjalsma","sequence":"first","affiliation":[{"name":"Laboratory of Genetic, Endocrine and Metabolic Diseases, Department of Laboratory Medicine"},{"name":"Hepcidinanalysis.com; Geert Grooteplein, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorine W","family":"Swinkels","sequence":"first","affiliation":[{"name":"Laboratory of Genetic, Endocrine and Metabolic Diseases, Department of Laboratory Medicine"},{"name":"Hepcidinanalysis.com; Geert Grooteplein, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2010,10,1]]},"reference":[{"key":"2020022704414125300_B1","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1182\/blood-2007-12-077388","article-title":"Forging a field: the golden age of iron 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spectrometry","volume":"110","author":"Murphy","year":"2007","journal-title":"Blood"},{"key":"2020022704414125300_B9","doi-asserted-by":"crossref","first-page":"4033","DOI":"10.1002\/rcm.3319","article-title":"Simple and sensitive quantification of bioactive peptides in biological matrices using liquid chromatography\/selected reaction monitoring mass spectrometry coupled with trichloroacetic acid clean-up","volume":"21","author":"Murao","year":"2007","journal-title":"Rapid Commun Mass Spectrom"},{"key":"2020022704414125300_B10","doi-asserted-by":"crossref","first-page":"e2706","DOI":"10.1371\/journal.pone.0002706","article-title":"Advances in quantitative hepcidin measurements by time-of-flight mass spectrometry","volume":"3","author":"Swinkels","year":"2008","journal-title":"PLoS ONE"},{"key":"2020022704414125300_B11","doi-asserted-by":"crossref","first-page":"4292","DOI":"10.1182\/blood-2008-02-139915","article-title":"Immunoassay for human serum 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disease","volume":"75","author":"Ashby","year":"2009","journal-title":"Kidney Int"},{"key":"2020022704414125300_B15","doi-asserted-by":"crossref","first-page":"e4581","DOI":"10.1371\/journal.pone.0004581","article-title":"A novel immunological assay for hepcidin quantification in human serum","volume":"4","author":"Koliaraki","year":"2009","journal-title":"PLoS ONE"},{"key":"2020022704414125300_B16","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.vascn.2009.02.004","article-title":"Development of a method for the sensitive and quantitative determination of hepcidin in human serum using LC-MS\/MS","volume":"59","author":"Li","year":"2009","journal-title":"J Pharmacol Toxicol Methods"},{"key":"2020022704414125300_B17","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1111\/j.1365-2141.2009.07758.x","article-title":"High-sensitive radioimmunoassay for human serum hepcidin","volume":"146","author":"Grebenchtchikov","year":"2009","journal-title":"Br J 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blood","volume":"113","author":"Peslova","year":"2009","journal-title":"Blood"},{"key":"2020022704414125300_B27","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1161\/CIRCHEARTFAILURE.108.790774","article-title":"Detection of endogenous B-type natriuretic peptide at very low concentrations in patients with heart failure","volume":"1","author":"Niederkofler","year":"2008","journal-title":"Circ Heart Fail"},{"key":"2020022704414125300_B28","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1111\/j.1365-2141.2009.07961.x","article-title":"Growth differentiation factor 15 in anaemia of chronic disease, iron deficiency anaemia and mixed type anaemia","volume":"148","author":"Theurl","year":"2010","journal-title":"Br J Haematol"},{"key":"2020022704414125300_B29","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1086\/595790","article-title":"Assessment of urinary concentrations of hepcidin provides novel insight into disturbances in iron homeostasis during malarial 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