{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:00:07Z","timestamp":1791414007723,"version":"4.3.3"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2013,6,1]],"date-time":"2013-06-01T00:00:00Z","timestamp":1370044800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,6,1]]},"abstract":"<jats:sec>\n                  <jats:title>BACKGROUND<\/jats:title>\n                  <jats:p>Measurement of serum thyroglobulin (Tg) is used to monitor patients after treatment for differentiated thyroid carcinoma (TC). Difficulty in using Tg as a biomarker of the recurrence of TC in many patients stems from the presence of endogenous anti-Tg autoantibodies (Tg-AAbs), which can interfere with immunoassays (IAs) and cause false-negative results.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>METHODS<\/jats:title>\n                  <jats:p>We enriched Tg from serum samples using rabbit polyclonal anti-Tg antiserum and protein precipitation. Unrelated proteins were partially depleted in the process. Enriched proteins were then denatured, reduced, and digested with trypsin after the addition of a winged internal standard peptide. A Tg-specific tryptic peptide was purified by immunoaffinity extraction and analyzed by 2-dimensional LC-MS\/MS. Instrument cycle time was 6.5 min per sample.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>RESULTS<\/jats:title>\n                  <jats:p>The lower limit of quantification was 0.5 ng\/mL (0.76 fmol\/mL dimer). Total imprecision of triplicate measurements in serum samples over 5 days was &amp;lt;10%. Comparison with a commercial IA using serum samples free of Tg-AAb (n = 73) showed Deming regression, IA = 1.00 * LC-MS\/MS \u2212 2.35, r = 0.982, standard error of the estimate (Sy|x) = 9.52. In a set of Tg-AAb\u2013positive samples that tested negative for Tg using IA (n = 71), concentrations determined by LC-MS\/MS were \u22650.5 ng\/mL in 23% of samples (median 1.2, range 0.7\u201311 ng\/mL).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>CONCLUSIONS<\/jats:title>\n                  <jats:p>The introduced method has acceptable performance characteristics for use in clinical diagnostic applications. The most substantial disagreement between methods was observed in Tg-AAb\u2013positive samples with concentrations &amp;lt;2 ng\/mL (determined with LC-MS\/MS). The affinity-assisted enrichment strategy used for Tg in this method should be applicable to other biomarkers that have endogenous autoantibodies.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1373\/clinchem.2012.195594","type":"journal-article","created":{"date-parts":[[2013,2,9]],"date-time":"2013-02-09T03:57:19Z","timestamp":1360382239000},"page":"982-990","source":"Crossref","is-referenced-by-count":181,"title":["Measurement of Thyroglobulin by Liquid Chromatography\u2013Tandem Mass Spectrometry in Serum and Plasma in the Presence of Antithyroglobulin Autoantibodies"],"prefix":"10.1093","volume":"59","author":[{"given":"Mark M","family":"Kushnir","sequence":"first","affiliation":[{"name":"ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT"},{"name":"Departments of Pathology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan L","family":"Rockwood","sequence":"first","affiliation":[{"name":"ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT"},{"name":"Departments of Pathology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William L","family":"Roberts","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dev","family":"Abraham","sequence":"first","affiliation":[{"name":"Medicine, University of Utah, Salt Lake City, UT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew N","family":"Hoofnagle","sequence":"first","affiliation":[{"name":"Departments of Laboratory Medicine and Medicine, University of Washington, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A Wayne","family":"Meikle","sequence":"first","affiliation":[{"name":"ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT"},{"name":"Departments of Pathology and"},{"name":"Medicine, University of Utah, Salt Lake City, UT"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,6,1]]},"reference":[{"key":"2020022705325729900_B1","doi-asserted-by":"crossref","first-page":"S492","DOI":"10.1007\/s00259-002-0847-9","article-title":"Follow-up of differentiated thyroid cancer","volume":"29","author":"Pacini","year":"2002","journal-title":"Eur J Nucl Med Mol Imaging"},{"key":"2020022705325729900_B2","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.cll.2004.01.001","article-title":"Thyroglobulin: a specific serum marker for the management of thyroid carcinoma","volume":"24","author":"Whitley","year":"2004","journal-title":"Clin Lab Med"},{"key":"2020022705325729900_B3","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1530\/EJE-10-0553","article-title":"Post-ablative serum thyroglobulin is an independent predictor of recurrence in low-risk differentiated thyroid carcinoma: a 16-year follow-up study","volume":"163","author":"Pelttari","year":"2010","journal-title":"Eur J Endocrinol"},{"key":"2020022705325729900_B4","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1084\/jem.66.2.251","article-title":"A quantitative theory of the precipitin reaction: VI. 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