{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:42:20Z","timestamp":1791409340503,"version":"4.3.3"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["LSHC-CT-2005-018911"],"award-info":[{"award-number":["LSHC-CT-2005-018911"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Research Council Investigator","award":["269081"],"award-info":[{"award-number":["269081"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,9,1]]},"abstract":"<jats:sec>\n                  <jats:title>BACKGROUND<\/jats:title>\n                  <jats:p>The incidence and number of circulating tumor cells (CTCs) in the peripheral blood of colorectal cancer patients are lower than in other cancer types, which may point to a particular biology of colorectal cancer affecting CTC detection.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>METHODS<\/jats:title>\n                  <jats:p>We detected CTCs in the peripheral and mesenteric blood of colorectal cancer patients by use of 2 independent technologies on the basis of different biological properties of colon cancer cells. Seventy-five patients diagnosed with localized (M0, n = 60) and metastatic (M1, n = 15) colorectal cancer were included. Peripheral and mesenteric blood samples were collected before tumor resection. We performed CTC enumeration with an EpCAM-independent enrichment method followed by the Epispot assay that detected only viable CK19-releasing CTCs. In parallel, we used the FDA-cleared EpCAM-dependent CellSearch\u00ae as the reference method.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>RESULTS<\/jats:title>\n                  <jats:p>The enumeration of CK19-releasing cells by the CK19-Epispot assay revealed viable CTCs in 27 of 41 (65.9%) and 41 of 74 (55.4%) (P = 0.04) patients in mesenteric and peripheral blood, respectively, whereas CellSearch detected CTCs in 19 of 34 (55.9%) and 20 of 69 (29.0%) (P = 0.0046) patients. In mesenteric blood, medians of 4 (range 0\u2013247) and 2.7 CTCs (range 0\u2013286) were found with Epispot and CellSearch (P = 0.2), respectively, whereas in peripheral blood, Epispot and CellSearch detected a median of 1.2 (range 0\u201392) and 0 CTCs (range 0\u2013147) (P = 0.002).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>CONCLUSIONS<\/jats:title>\n                  <jats:p>A considerable portion of viable CTCs detectable by the Epispot assay are trapped in the liver as the first filter organ in CRC patients.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1373\/clinchem.2013.202846","type":"journal-article","created":{"date-parts":[[2013,5,21]],"date-time":"2013-05-21T21:50:59Z","timestamp":1369173059000},"page":"1384-1392","source":"Crossref","is-referenced-by-count":204,"title":["Capture of Viable Circulating Tumor Cells in the Liver of Colorectal Cancer 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Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Alix-Panabi\u00e8res","sequence":"first","affiliation":[{"name":"University Institute of Clinical Research UM1, EA2415, Epidemiology, Biostatistics & Public Health, Montpellier, France"},{"name":"Cell and Tissue Biopathology of Tumors Department, University Medical Centre, Saint Eloi Hospital, Montpellier, France"},{"name":"Institute of Research in Biotherapy, Laboratory of Rare Human Circulating Cells, University Medical Centre, Saint-Eloi Hospital, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,9,1]]},"reference":[{"key":"2020022705334960500_B1","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1146\/annurev-med-062310-094219","article-title":"Circulating tumor cells and circulating tumor DNA","volume":"63","author":"Alix-Panabi\u00e8res","year":"2012","journal-title":"Annu Rev 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