{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T19:26:28Z","timestamp":1789241188843,"version":"build-2803163510"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>BACKGROUND<\/jats:title>\n                  <jats:p>Group B streptococcal infections are the leading cause of sepsis and meningitis in newborns. A rapid and reliable method for the detection of this pathogen at the time of delivery is needed for the early treatment of neonates. Isothermal amplification techniques such as recombinase polymerase amplification have advantages relative to PCR in terms of the speed of reaction and simplicity.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>METHODS<\/jats:title>\n                  <jats:p>We studied the clinical performance of recombinase polymerase amplification for the screening of group B streptococci in vaginal\/anal samples from 50 pregnant women. We also compared the limit of detection and the analytical specificity of this isothermal assay to real-time PCR (RT-PCR).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>RESULTS<\/jats:title>\n                  <jats:p>Compared to RT-PCR, the recombinase polymerase amplification assay showed a clinical sensitivity of 96% and a clinical specificity of 100%. The limit of detection was 98 genome copies and the analytical specificity was 100% for a panel of 15 bacterial and\/or fungal strains naturally found in the vaginal\/anal flora. Time-to-result for the recombinase polymerase amplification assay was &amp;lt;20 min compared to 45 min for the RT-PCR assay; a positive sample could be detected as early as 8 min.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>CONCLUSIONS<\/jats:title>\n                  <jats:p>We demonstrate the potential of isothermal recombinase polymerase amplification assay as a clinically useful molecular diagnostic tool that is simple and faster than PCR\/RT-PCR. Recombinase polymerase amplification offers great potential for nucleic acid\u2013based diagnostics at the point of care.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1373\/clinchem.2013.213504","type":"journal-article","created":{"date-parts":[[2014,1,24]],"date-time":"2014-01-24T22:38:37Z","timestamp":1390603117000},"page":"660-666","source":"Crossref","is-referenced-by-count":43,"title":["Isothermal Recombinase Polymerase Amplification Assay Applied to the Detection of Group B Streptococci in Vaginal\/Anal Samples"],"prefix":"10.1093","volume":"60","author":[{"given":"Rana K","family":"Daher","sequence":"first","affiliation":[{"name":"Centre de recherche du CHU de Qu\u00e9bec, Centre de recherche en infectiologie de l'Universit\u00e9 Laval (CRI), Quebec, Canada"},{"name":"D\u00e9partement de microbiologie-immunologie, facult\u00e9 de m\u00e9decine, Universit\u00e9 Laval, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gale","family":"Stewart","sequence":"first","affiliation":[{"name":"Centre de recherche du CHU de Qu\u00e9bec, Centre de recherche en infectiologie de l'Universit\u00e9 Laval (CRI), Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurice","family":"Boissinot","sequence":"first","affiliation":[{"name":"Centre de recherche du CHU de Qu\u00e9bec, Centre de recherche en infectiologie de l'Universit\u00e9 Laval (CRI), Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel G","family":"Bergeron","sequence":"first","affiliation":[{"name":"Centre de recherche du CHU de Qu\u00e9bec, Centre de recherche en infectiologie de l'Universit\u00e9 Laval (CRI), Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,4,1]]},"reference":[{"key":"2020022705442255600_B1","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1055\/s-0032-1332804","article-title":"Early onset neonatal group B streptococcal sepsis","volume":"30","author":"Oh","year":"2013","journal-title":"Am J Perinatol"},{"key":"2020022705442255600_B2","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1056\/NEJM198606263142603","article-title":"Prevention of early-onset neonatal group B streptococcal disease with selective intrapartum chemoprophylaxis","volume":"314","author":"Boyer","year":"1986","journal-title":"N Engl J Med"},{"key":"2020022705442255600_B3","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/S1413-8670(11)70199-4","article-title":"Group B streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women","volume":"15","author":"de-Paris","year":"2011","journal-title":"Braz J Infect Dis"},{"key":"2020022705442255600_B4","first-page":"1","article-title":"Prevention of perinatal group B streptococcal disease. 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