{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:57:04Z","timestamp":1791118624146,"version":"4.1.0"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2014,5,1]],"date-time":"2014-05-01T00:00:00Z","timestamp":1398902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The goal of this study was to establish a plasma microRNA profile by use of next-generation sequencing that could aid in assessment of patient prognosis in nasopharyngeal carcinoma (NPC).<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Two panels of NPC patients and healthy controls (HCs) were recruited for this study. We used deep sequencing to screen plasma microRNAs. Differentially expressed microRNAs were verified by quantitative real-time PCR (qPCR). Kaplan\u2013Meier survival analysis with the log-rank test was used to compare overall survival (OS) and progression-free survival (PFS) between groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Twenty-three plasma miRNAs with differential expression levels were selected for qPCR analysis on an independent set including 100 NPC patients and 55 HCs. NPC patients with low concentrations of miR-483\u20135p and miR-103 had better prognosis for 5-year OS than those with high concentrations (87.5% vs 55.8%, P &amp;lt; 0.001; 80.9% vs 62.3%, P = 0.031). Those with low concentrations of miR-29a and let-7c had poorer prognosis (54.8% vs 82.8%, P = 0.002; 56.3% vs 84.6%, P = 0.001). A 3-signature miRNA integrated with clinical stage was further identified in an independent set. We calculated a prognostic index score and classified patients into low-, medium-, and high-risk groups. Five-year OS among the 3 groups was significantly different (90.9%, 66.7%, and 23.8%; P &amp;lt; 0.001). By multivariate analysis, a high-risk score was the most significantly unfavorable prognostic factor independent of other clinical variables (P &amp;lt; 0.001, hazard ratio = 15.1, 95% CI = 5.2\u201343.9).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Differentially expressed plasma miRNAs as identified by next-generation sequencing can be helpful for predicting survival in NPC patients.<\/jats:p><\/jats:sec>","DOI":"10.1373\/clinchem.2013.214213","type":"journal-article","created":{"date-parts":[[2014,2,22]],"date-time":"2014-02-22T05:57:42Z","timestamp":1393048662000},"page":"773-782","source":"Crossref","is-referenced-by-count":48,"title":["Profiling Plasma MicroRNA in Nasopharyngeal Carcinoma with Deep Sequencing"],"prefix":"10.1093","volume":"60","author":[{"given":"Hai-Yun","family":"Wang","sequence":"first","affiliation":[{"name":"Departments of Molecular Diagnostics and"},{"name":"Department of Medical Epidemiology and Biostatistics, Karolinska 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