{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T14:24:58Z","timestamp":1758637498090},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T00:00:00Z","timestamp":1454284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>BACKGROUND<\/jats:title>\n                  <jats:p>Multiantibiotic-resistant bacteria pose a threat to patients and place an economic burden on health care systems. Carbapenem-resistant bacilli and extended-spectrum \u03b2-lactamase (ESBL) producers drive the need to screen infected and colonized patients for patient management and infection control.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>METHODS<\/jats:title>\n                  <jats:p>We describe a multiplex microfluidic PCR test for perianal swab samples (Acuitas\u00ae MDRO Gene Test, OpGen) that detects the vancomycin-resistance gene vanA plus hundreds of gene subtypes from the carbapenemase and ESBL families Klebsiella pneumoniae carbapenemase (KPC), New Delhi metallo-\u03b2-lactamase (NDM), Verona integron-mediated metallo-\u03b2-lactamase (VIM), imipenemase metallo-\u03b2-lactamase (IMP), OXA-23, OXA-48, OXA-51, CTX-M-1, and CTX-M-2, regardless of the bacterial species harboring the antibiotic resistance.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>RESULTS<\/jats:title>\n                  <jats:p>Analytical test sensitivity per perianal swab is 11\u2013250 CFU of bacteria harboring the antibiotic resistance genes. Test throughput is 182 samples per test run (1820 antibiotic resistance gene family results). We demonstrate reproducible test performance and 100% gene specificity for 265 clinical bacterial organisms harboring a variety of antibiotic resistance genes.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>CONCLUSIONS<\/jats:title>\n                  <jats:p>The Acuitas MDRO Gene Test is a sensitive, specific, and high-throughput test to screen colonized patients and diagnose infections for several antibiotic resistance genes directly from perianal swab samples, regardless of the bacterial species harboring the resistance genes.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1373\/clinchem.2015.246371","type":"journal-article","created":{"date-parts":[[2015,12,5]],"date-time":"2015-12-05T04:50:54Z","timestamp":1449291054000},"page":"353-359","source":"Crossref","is-referenced-by-count":9,"title":["Analytical Performance of Multiplexed Screening Test for 10 Antibiotic Resistance Genes from Perianal Swab Samples"],"prefix":"10.1093","volume":"62","author":[{"given":"G Terrance","family":"Walker","sequence":"first","affiliation":[{"name":"OpGen, Gaithersburg, MD"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tony J","family":"Rockweiler","sequence":"first","affiliation":[{"name":"OpGen, Gaithersburg, MD"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rossio K","family":"Kersey","sequence":"first","affiliation":[{"name":"OpGen, Gaithersburg, MD"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly L","family":"Frye","sequence":"first","affiliation":[{"name":"OpGen, Gaithersburg, MD"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan R","family":"Mitchner","sequence":"first","affiliation":[{"name":"OpGen, Gaithersburg, MD"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas R","family":"Toal","sequence":"first","affiliation":[{"name":"OpGen, Gaithersburg, MD"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Quan","sequence":"first","affiliation":[{"name":"OpGen, Gaithersburg, MD"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2016,2,1]]},"reference":[{"key":"2020022605231817500_B1","unstructured":"Centers for Disease Control and Prevention. Antibiotic resistance threats in the United States, 2013. http:\/\/www.cdc.gov\/drugresistance\/threat-report-2013\/index.html. (Accessed November 2014)."},{"key":"2020022605231817500_B2","doi-asserted-by":"crossref","first-page":"2596","DOI":"10.1128\/JCM.00654-12","article-title":"Rectal screening for Klebsiella pneumoniae carbapenemases: comparison of real-time PCR and culture using two selective screening agar plates","volume":"50","author":"Singh","year":"2012","journal-title":"J Clin Microbiol"},{"key":"2020022605231817500_B3","unstructured":"Centers for Disease Control and Prevention. 2012 CRE Toolkit\u2014Guidance for control of carbapenem-resistant Enterobacteriaceae (CRE). http:\/\/www.cdc.gov\/hai\/organisms\/cre\/cre-toolkit\/index.html. 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Carbapenem-resistant Enterobacteriaceae (CRE) control and prevention toolkit. Rockville (MD): Agency for Healthcare Research and Quality (AHRQ); 2014 Apr. Contract No. 290-2006-0012-l. AHRQ Publication No. 14-0028. Available from: http:\/\/www.ahrq.gov\/professionals\/quality-patient-safety\/patient-safety-resources\/resources\/cretoolkit\/index.html"},{"key":"2020022605231817500_B15","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1093\/cid\/ciu847","article-title":"Surveillance cultures growing carbapenem-resistant Acinetobacter baumannii predict the development of clinical infections: a cohort study","volume":"60","author":"Latibeaudiere","year":"2015","journal-title":"Clin Infect Dis"},{"key":"2020022605231817500_B16","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1086\/662620","article-title":"A multifaceted intervention strategy for eradication of a hospital-wide outbreak caused by carbapenem-resistant Klebsiella pneumoniae in Southern Israel","volume":"32","author":"Borer","year":"2011","journal-title":"Infect Control Hosp Epidemiol"},{"key":"2020022605231817500_B17","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.ijid.2014.05.012","article-title":"Blood stream infections due to OXA-48-like carbapenemase-producing Enterobacteriaceae: treatment and survival","volume":"26","author":"Balkan","year":"2014","journal-title":"Int J Infect Dis"}],"container-title":["Clinical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/clinchem\/article-pdf\/62\/2\/353\/32643710\/clinchem0353.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/clinchem\/article-pdf\/62\/2\/353\/32643710\/clinchem0353.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,2,26]],"date-time":"2020-02-26T10:24:08Z","timestamp":1582712648000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/clinchem\/article\/62\/2\/353\/5611850"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,2,1]]},"references-count":17,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2016,2,1]]},"published-print":{"date-parts":[[2016,2,1]]}},"URL":"https:\/\/doi.org\/10.1373\/clinchem.2015.246371","relation":{},"ISSN":["0009-9147","1530-8561"],"issn-type":[{"value":"0009-9147","type":"print"},{"value":"1530-8561","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2016,2,1]]},"published":{"date-parts":[[2016,2,1]]}}}