{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:31:40Z","timestamp":1790721100191,"version":"4.1.0"},"reference-count":83,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"vor","delay-in-days":2,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>BACKGROUND<\/jats:title>\n                  <jats:p>The PCR and its variant, quantitative PCR (qPCR), have revolutionized the practice of clinical microbiology. Continued advancements in PCR have led to a new derivative, digital PCR (dPCR), which promises to address certain limitations inherent to qPCR.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>CONTENT<\/jats:title>\n                  <jats:p>Here we highlight the important technical differences between qPCR and dPCR, and the potential advantages and disadvantages of each. We then review specific situations in which dPCR has been implemented in clinical microbiology and the results of such applications. Finally, we attempt to place dPCR in the context of other emerging technologies relevant to the clinical laboratory, including next-generation sequencing.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>SUMMARY<\/jats:title>\n                  <jats:p>dPCR offers certain clear advantages over traditional qPCR, but these are to some degree offset by limitations of the technology, at least as currently practiced. Laboratories considering implementation of dPCR should carefully weigh the potential advantages and disadvantages of this powerful technique for each specific application planned.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1373\/clinchem.2019.304048","type":"journal-article","created":{"date-parts":[[2019,11,8]],"date-time":"2019-11-08T23:15:13Z","timestamp":1573254913000},"page":"117-123","source":"Crossref","is-referenced-by-count":151,"title":["Digital PCR\u2014An Emerging Technology with Broad Applications in Microbiology"],"prefix":"10.1093","volume":"66","author":[{"given":"Stephen J","family":"Salipante","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, University of Washington, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith R","family":"Jerome","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, University of Washington, Seattle, WA"},{"name":"Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,12,30]]},"reference":[{"key":"2020010518580217100_B1","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/S1473-3099(04)01044-8","article-title":"PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings","volume":"4","author":"Yang","year":"2004","journal-title":"Lancet Infect Dis"},{"key":"2020010518580217100_B2","doi-asserted-by":"crossref","first-page":"3133","DOI":"10.1128\/JCM.41.7.3133-3141.2003","article-title":"Evaluation of real-time PCR versus PCR with liquid-phase hybridization for detection of enterovirus RNA in cerebrospinal fluid","volume":"41","author":"Lai","year":"2003","journal-title":"J Clin 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