{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T05:27:01Z","timestamp":1787981221211,"version":"build-2784847793"},"reference-count":8,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2016,11,1]],"date-time":"2016-11-01T00:00:00Z","timestamp":1477958400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,11,1]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Measurement of amino acids in dried blood spots has been extensively used for the detection of newborns with various inborn errors of amino acid metabolism including phenylketonuria (PKU) and maple syrup urine disease (MSUD). Whereas blood spot amino acid measurement has been invaluable for initial diagnosis, the relative insensitivity of blood spot measurement has found limited use in lifelong monitoring of patients with these disorders. The work described here outlines the evaluation of blood spot amino acid analysis using ultra-performance liquid chromatography (UPLC\u00a9) for use in follow-up testing.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Dried blood spot amino acids were derivatized with a proprietary AccQTag\u00ae reagent and separated using UPLC. Plasma amino acids from dried bloods spots were obtained from 318 patient samples and compared to corresponding plasma samples measured using the same UPLC method.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Dried blood spot amino acid concentrations were highly correlated but negatively biased vs plasma concentrations. Interassay imprecision studies using UPLC demonstrated a %CV for phenylalanine of 4.81%\u201316.07%, tyrosine 5.62%\u201320.16%, valine 4.23%\u201315.46%, leucine 8.3%\u201315.3%, and isoleucine 4.25%\u201316.80%. Intraassay imprecision studies using UPLC demonstrated a %CV for phenylalanine of 0.42%\u20133.4%, tyrosine 1.6%\u20137.85%, valine 0.14%\u20131.84%, leucine 0.28%\u20132.01%, and isoleucine 0.6%\u20132.65%. Blood spot amino acid concentrations were stable for at least 3 days at temperatures up to 65 \u00b0C.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n This UPLC-based method can reliably measure clinically significant amino acids in dried blood spots.<\/jats:p>\n <\/jats:sec>","DOI":"10.1373\/jalm.2016.020289","type":"journal-article","created":{"date-parts":[[2018,7,23]],"date-time":"2018-07-23T22:25:56Z","timestamp":1532384756000},"page":"271-279","source":"Crossref","is-referenced-by-count":13,"title":["A Quantitative Method for the Measurement of Dried Blood Spot Amino Acids Using Ultra-Performance Liquid Chromatography"],"prefix":"10.1093","volume":"1","author":[{"given":"Kaitlyn","family":"Bloom","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gail","family":"Ditewig Meyers","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J","family":"Bennett","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA"},{"name":"Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,11,1]]},"reference":[{"key":"2022020105013329200_B1","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.ymgme.2014.05.006","article-title":"Nutrition management guideline for maple syrup urine disease: an evidence- and consensus-based approach","volume":"112","author":"Frazier","year":"2014","journal-title":"Mol Genet Metab"},{"key":"2022020105013329200_B2","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1258\/acb.2011.010283","article-title":"An improved ultra performance liquid chromatography-tandem mass spectrometry method for the determination of alloisoleucine and branched chain amino acids in dried blood samples","volume":"48","author":"Alodaib","year":"2011","journal-title":"Ann Clin Biochem"},{"key":"2022020105013329200_B3","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1373\/clinchem.2007.098434","article-title":"Second-tier test for quantification of alloisoleucine and branched-chain amino acids in dried blood spots to improve newborn screening for maple syrup urine disease (MSUD)","volume":"54","author":"Oglesbee","year":"2008","journal-title":"Clin Chem"},{"key":"2022020105013329200_B4","unstructured":"CAP. 2015 Proficiency Testing Manual. Available from: http:\/\/www.cap.org\/apps\/docs\/proficiency_testing\/surveys-excel-manual.pdf (Accessed January 2016)."},{"key":"2022020105013329200_B5","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1258\/000456307781646012","article-title":"A rapid ultra performance liquid chromatography tandem mass spectrometric method for measuring amino acids associated with maple syrup urine disease, tyrosinaemia and phenylketonuria","volume":"44","author":"Freeto","year":"2007","journal-title":"Ann Clin Biochem"},{"key":"2022020105013329200_B6","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1007\/s00216-012-6194-1","article-title":"A rapid UPLC-MS\/MS method for simultaneous separation of 48 acylcarnitines in dried blood spots and plasma useful as a second-tier test for expanded newborn screening","volume":"404","author":"Gucciardi","year":"2012","journal-title":"Anal Bioanal Chem"},{"key":"2022020105013329200_B7","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.jpba.2015.07.008","article-title":"Direct tandem mass spectrometric analysis of amino acids in plasma using fluorous derivatization and monolithic solid-phase purification","volume":"115","author":"Tamashima","year":"2015","journal-title":"J Pharm Biomed Anal"},{"key":"2022020105013329200_B8","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1515\/CCLM.2011.200","article-title":"Measurement of plasma amino acids by Ultraperformance\u00ae Liquid Chromatography","volume":"49","author":"Narayan","year":"2011","journal-title":"Clin Chem Lab Med"}],"container-title":["The Journal of Applied Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jalm\/article-pdf\/1\/3\/271\/42352478\/1_1_3_271.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jalm\/article-pdf\/1\/3\/271\/42352478\/1_1_3_271.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T05:02:04Z","timestamp":1643691724000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jalm\/article\/1\/3\/271\/5587323"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,1]]},"references-count":8,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2016,11,1]]},"published-print":{"date-parts":[[2016,11,1]]}},"URL":"https:\/\/doi.org\/10.1373\/jalm.2016.020289","relation":{},"ISSN":["2576-9456","2475-7241"],"issn-type":[{"value":"2576-9456","type":"print"},{"value":"2475-7241","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2016,11,1]]},"published":{"date-parts":[[2016,11,1]]}}}