{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T14:37:05Z","timestamp":1773758225795,"version":"3.50.1"},"reference-count":52,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[1998,6,1]],"date-time":"1998-06-01T00:00:00Z","timestamp":896659200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[1998,6,1]],"date-time":"1998-06-01T00:00:00Z","timestamp":896659200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","journal.chestnet.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Chest"],"published-print":{"date-parts":[[1998,6]]},"DOI":"10.1378\/chest.113.6.1526","type":"journal-article","created":{"date-parts":[[2008,1,22]],"date-time":"2008-01-22T17:31:17Z","timestamp":1201023077000},"page":"1526-1532","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":59,"title":["Detection of Occult Micrometastases in Non-Small Cell Lung Carcinoma by Reverse Transcriptase-Polymerase Chain Reaction"],"prefix":"10.1016","volume":"113","author":[{"given":"Christopher T.","family":"Salerno","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Frizelle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gloria A.","family":"Niehans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sam B.","family":"Ho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Molathi","family":"Jakkula","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"Kratzke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael A.","family":"Maddaus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1378\/chest.113.6.1526_bib1","doi-asserted-by":"crossref","first-page":"1710","DOI":"10.1378\/chest.111.6.1710","article-title":"Revisions in the International System for Staging Lung Cancer.","volume":"111","author":"Mountain","year":"1997","journal-title":"Chest"},{"key":"10.1378\/chest.113.6.1526_bib2","first-page":"3415","article-title":"Rb and pl6INK4a expression in resected non-small cell lung tumors.","volume":"56","author":"Kratzke","year":"1996","journal-title":"Cancer Res"},{"key":"10.1378\/chest.113.6.1526_bib3","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1016\/S0140-6736(96)91203-9","article-title":"Frequency and prognostic significance of isolated tumor cells in bone marrow of patients with non-small-cell lung cancer without overt metastases.","volume":"347","author":"Pantel","year":"1996","journal-title":"Lancet"},{"key":"10.1378\/chest.113.6.1526_bib4","first-page":"1027","article-title":"Immunocytological detection of bone marrow micrometastases in operable non-small cell lung cancer.","volume":"53","author":"Pantel","year":"1993","journal-title":"Cancer Res"},{"key":"10.1378\/chest.113.6.1526_bib5","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1200\/JCO.1994.12.9.1827","article-title":"Immunohistochemical assessment of individual tumor cells in lymph nodes of patients with non-small cell lung cancer.","volume":"12","author":"Passlick","year":"1994","journal-title":"J Clin Oncol"},{"key":"10.1378\/chest.113.6.1526_bib6","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/0003-4975(95)01012-2","article-title":"Detection of disseminated lung cancer cells in lymph nodes: impact on staging and prognosis.","volume":"61","author":"Passlick","year":"1996","journal-title":"Ann Thorac Surg"},{"key":"10.1378\/chest.113.6.1526_bib7","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/jnci\/85.6.493","article-title":"Frequency and distribution of occult micrometastases in lymph nodes of patients with non-small cell lung carcinoma.","volume":"85","author":"Chen","year":"1993","journal-title":"J Natl Cancer Inst"},{"key":"10.1378\/chest.113.6.1526_bib8","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/0952-7915(92)90041-C","article-title":"Monoclonal antibodies in the detection and therapy of micrometastatic epithelial cancers.","volume":"4","author":"Riethmuller","year":"1992","journal-title":"Curr Opin Immunol"},{"key":"10.1378\/chest.113.6.1526_bib9","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/S0090-4295(94)80267-X","article-title":"Immunohistochemical detection of prostate cancer pelvic lymph node micrometastases: correlation to preoperative prostate-specific antigen.","volume":"43","author":"Moul","year":"1994","journal-title":"Urology"},{"key":"10.1378\/chest.113.6.1526_bib10","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/BF00179388","article-title":"Micrometastases from squamous cell carcinoma in neck dissection specimens.","volume":"249","author":"van","year":"1992","journal-title":"Eur Arch Otorhinolaryngol"},{"key":"10.1378\/chest.113.6.1526_bib11","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1200\/JCO.1990.8.5.831","article-title":"Epithelial tumor cells in bone marrow of patients with colorectal cancer: immunohistochemical detection, phenotypic characterization, and prognostic significance.","volume":"8","author":"Schlimok","year":"1990","journal-title":"J Clin Oncol"},{"key":"10.1378\/chest.113.6.1526_bib12","doi-asserted-by":"crossref","first-page":"1461","DOI":"10.1016\/0277-5379(91)90032-9","article-title":"Micrometastatic tumour cells in bone marrow of patients with gastric cancer: methodological aspects of detection and prognostic significance.","volume":"27","author":"Schlimok","year":"1991","journal-title":"Eur J Cancer"},{"key":"10.1378\/chest.113.6.1526_bib13","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1002\/path.1711720205","article-title":"The prognostic significance of immunohistochemically detected lymph node micrometastases in colorectal carcinoma.","volume":"172","author":"Jeffers","year":"1994","journal-title":"J Pathol"},{"key":"10.1378\/chest.113.6.1526_bib14","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1002\/1097-0142(19940201)73:3<563::AID-CNCR2820730311>3.0.CO;2-D","article-title":"Identification of occult micrometastases in pericolic lymph nodes of Dukes' B colorectal cancer patients using monoclonal antibodies against cytokeratin and CC49.","volume":"73","author":"Greenson","year":"1994","journal-title":"Cancer"},{"key":"10.1378\/chest.113.6.1526_bib15","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1002\/j.1098-2388.1993.tb00022.x","article-title":"Identification of submicroscopic lymph node metastases in patients with malignant melanoma.","volume":"9","author":"Heller","year":"1993","journal-title":"Semin Surg Oncol"},{"key":"10.1378\/chest.113.6.1526_bib16","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1001\/archsurg.1991.01410360025005","article-title":"Detection of submicroscopic lymph node metastases in patients with melanoma.","volume":"126","author":"Heller","year":"1991","journal-title":"Arch Surg"},{"key":"10.1378\/chest.113.6.1526_bib17","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1038\/bjc.1986.219","article-title":"The immunohistochemical detection of lymph node metastases from infiltrating lobular carcinoma of the breast.","volume":"54","author":"Bussolati","year":"1986","journal-title":"Br J Cancer"},{"key":"10.1378\/chest.113.6.1526_bib18","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1177\/172460089100600305","article-title":"Demonstration of mucinous-like carcinoma-associated antigen in bone marrow and lymph node biopsies from patients with breast carcinoma.","volume":"6","author":"Saccani","year":"1991","journal-title":"Int J Biol Markers"},{"key":"10.1378\/chest.113.6.1526_bib19","first-page":"65","article-title":"Detection of occult metastatic lobular carcinoma in axillary lymph nodes using anticytokeratin monoclonal antibodies.","volume":"56","author":"Lachman","year":"1992","journal-title":"Conn Med"},{"key":"10.1378\/chest.113.6.1526_bib20","first-page":"15","article-title":"Detection of micrometastases from primary breast cancer.","volume":"34","author":"Courtemanche","year":"1991","journal-title":"Can J Surg"},{"key":"10.1378\/chest.113.6.1526_bib21","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1002\/jso.2930470108","article-title":"Detection of micrometastatic tumor cells in bone marrow of breast carcinoma patients.","volume":"47","author":"Singletary","year":"1991","journal-title":"J Surg Oncol"},{"key":"10.1378\/chest.113.6.1526_bib22","first-page":"171","article-title":"Immunohistochemical detection and significance of axillary lymph nodes in breast carcinoma.","volume":"15","author":"Elson","year":"1993","journal-title":"Anal Quant Cytol Histol"},{"key":"10.1378\/chest.113.6.1526_bib23","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1016\/S0959-8049(05)80120-0","article-title":"A preliminary report on the usefulness of monoclonal antibodies to CA-153 and MCA in the detection of micrometastases in axillary lymph nodes draining primary breast carcinoma.","volume":"28","author":"Byrne","year":"1992","journal-title":"Eur J Cancer"},{"key":"10.1378\/chest.113.6.1526_bib24","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1200\/JCO.1991.9.10.1749","article-title":"Prediction of early relapse with operable breast cancer by detection of occult bone marrow micrometastases.","volume":"9","author":"Cote","year":"1991","journal-title":"J Clin Oncol"},{"key":"10.1378\/chest.113.6.1526_bib25","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1093\/jnci\/85.17.1419","article-title":"Differential expression of proliferation associated molecules in individual micrometastatic carcinoma cells.","volume":"85","author":"Pantel","year":"1993","journal-title":"J Natl Cancer Inst"},{"key":"10.1378\/chest.113.6.1526_bib26","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/0140-6736(92)92230-D","article-title":"Prognostic significance of micrometastatic tumour cells in bone marrow of colorectal cancer patients.","volume":"340","author":"Lindermann","year":"1992","journal-title":"Lancet"},{"key":"10.1378\/chest.113.6.1526_bib27","first-page":"3417","article-title":"Detection of cancer micrometastases in lymph nodes by reverse transcriptasepolymerase chain reaction.","volume":"55","author":"Mori","year":"1995","journal-title":"Cancer Res"},{"key":"10.1378\/chest.113.6.1526_bib28","first-page":"649","article-title":"Detection of breast cancer micrometastases in axillary lymph nodes by means of reverse transcriptase-polymerase chain reaction.","volume":"148","author":"Noguchi","year":"1996","journal-title":"Am J Pathol"},{"key":"10.1378\/chest.113.6.1526_bib29","doi-asserted-by":"crossref","first-page":"1595","DOI":"10.1002\/1097-0142(19940901)74:5<1595::AID-CNCR2820740516>3.0.CO;2-L","article-title":"The detection of breast carcinoma micrometastases in axillary lymph nodes by means of reverse transcriptase-polymerase chain reaction.","volume":"74","author":"Noguchi","year":"1994","journal-title":"Cancer"},{"key":"10.1378\/chest.113.6.1526_bib30","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1200\/JCO.1994.12.3.475","article-title":"Sensitive detection of occult breast cancer by the reverse transcriptase polymerase chain reaction.","volume":"12","author":"Datta","year":"1994","journal-title":"J Clin Oncol"},{"key":"10.1378\/chest.113.6.1526_bib31","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1097\/00000658-199412000-00010","article-title":"Detection of submicroscopic lymph node metastases with polymerase chain reaction in patients with malignant melanoma.","volume":"6","author":"Wang","year":"1994","journal-title":"Ann Surg"},{"key":"10.1378\/chest.113.6.1526_bib32","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1002\/ijc.2910640312","article-title":"Can a test for E6\/E7 transcripts of human papillomavirus type 16 serve as a diagnostic tool for the detection of micrometastasis in cervical cancer?","volume":"64","author":"Czegledy","year":"1995","journal-title":"Int J Cancer"},{"key":"10.1378\/chest.113.6.1526_bib33","first-page":"2986","article-title":"Detection of breast cancer micrometastases in axillary lymph nodes by using polymerase chain reaction.","volume":"54","author":"Schoenfeld","year":"1994","journal-title":"Cancer Res"},{"key":"10.1378\/chest.113.6.1526_bib34","first-page":"2640","article-title":"Prostate-specific antigen messenger RNA is expressed in non-prostate cells: implications for detection of micrometastases.","volume":"55","author":"Smith","year":"1995","journal-title":"Cancer Res"},{"key":"10.1378\/chest.113.6.1526_bib35","doi-asserted-by":"crossref","first-page":"2533","DOI":"10.1002\/1097-0142(19941101)74:9<2533::AID-CNCR2820740922>3.0.CO;2-D","article-title":"Identification of bone marrow micrometastases in patients with prostate cancer.","volume":"74","author":"Wood","year":"1994","journal-title":"Cancer"},{"key":"10.1378\/chest.113.6.1526_bib36","first-page":"5350","article-title":"Detection of micrometastatic prostate cancer cells in lymph nodes by reverse transcriptase-polymerase reaction.","volume":"53","author":"Deguchi","year":"1993","journal-title":"Cancer Res"},{"key":"10.1378\/chest.113.6.1526_bib37","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/0378-1119(94)00522-T","article-title":"Diagnosis of micrometastases by the amplification of tissue specific genes.","volume":"159","author":"Neumaier","year":"1995","journal-title":"Gene"},{"key":"10.1378\/chest.113.6.1526_bib38","doi-asserted-by":"crossref","first-page":"2109","DOI":"10.1200\/JCO.1995.13.8.2109","article-title":"Detection of occult melanoma cells in with a multiple-marker polymerase chain reaction assay.","volume":"13","author":"Hoon","year":"1995","journal-title":"J Clin Oncol"},{"key":"10.1378\/chest.113.6.1526_bib39","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1177\/42.4.7510319","article-title":"Sensitivity of immunohistochemistry and polymerase chain reaction in detecting prostate cancer cells in bone marrow.","volume":"42","author":"Wood","year":"1994","journal-title":"J Histochem Cytochem"},{"key":"10.1378\/chest.113.6.1526_bib40","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1165\/ajrcmb\/7.2.161","article-title":"Characterization of the mucin differentiation in human lung adenocarcinoma cell lines.","volume":"7","author":"Yang","year":"1992","journal-title":"Am J Respir Cell Biol"},{"key":"10.1378\/chest.113.6.1526_bib41","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1177\/172460089400900414","article-title":"Mucin gene expression in lung cancer tissues.","volume":"9","author":"Bombardieri","year":"1994","journal-title":"Int J Biol Markers"},{"key":"10.1378\/chest.113.6.1526_bib42","first-page":"641","article-title":"Heterogeneity of mucin gene expression in normal and neoplastic tissues.","volume":"53","author":"Ho","year":"1993","journal-title":"Cancer Res"},{"key":"10.1378\/chest.113.6.1526_bib43","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1177\/41.10.8245407","article-title":"Expression of human mucin genes in respiratory, digestive and reproductive tracts ascertained by in situ hybridization.","volume":"41","author":"Audie","year":"1993","journal-title":"J Histochem Cytochem"},{"key":"10.1378\/chest.113.6.1526_bib44","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1242\/jcs.107.2.413","article-title":"Developmental expression of mucin genes MUC1 and MUC2.","volume":"107","author":"Chambers","year":"1994","journal-title":"J Cell Sci"},{"key":"10.1378\/chest.113.6.1526_bib45","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1164\/arrd.1983.127.5.659","article-title":"American Thoracic Society: Clinical staging of primary lung cancer.","volume":"127","year":"1983","journal-title":"Am Rev Respir Dis"},{"key":"10.1378\/chest.113.6.1526_bib46","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1016\/S0006-291X(05)80888-5","article-title":"Structure and expression of the human polymorphic epithelial mucin gene: an expressed VNTR unit.","volume":"173","author":"Lancaster","year":"1990","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1378\/chest.113.6.1526_bib47","first-page":"1026","article-title":"Relationship of pancreatic cancer apomucin to mammary and intestinal apomucins.","volume":"51","author":"Byrd","year":"1991","journal-title":"Cancer Res"},{"key":"10.1378\/chest.113.6.1526_bib48","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1097\/00000658-199522240-00001","article-title":"Detection of occult bone marrow micrometastases in patients with operable lung carcinoma.","volume":"222","author":"Cote","year":"1995","journal-title":"Ann Surg"},{"key":"10.1378\/chest.113.6.1526_bib49","first-page":"6545","article-title":"Immunocytological detection of bone marrow disease at clinical remission predicts metastatic relapse in non-small cell lung cancer.","volume":"50","author":"Leonard","year":"1990","journal-title":"Cancer Res"},{"key":"10.1378\/chest.113.6.1526_bib50","doi-asserted-by":"crossref","first-page":"2769","DOI":"10.1200\/JCO.1995.13.11.2769","article-title":"Low specificity of cytokeratin 19 reverse transcriptase-polymerase chain reaction analyses for detection of hematogenous lung cancer dissemination.","volume":"13","author":"Krismann","year":"1995","journal-title":"J Clin Oncol"},{"key":"10.1378\/chest.113.6.1526_bib51","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1093\/jnci\/86.9.673","article-title":"A randomized trial comparing perioperative chemotherapy and surgery with surgery alone in resectable stage IIIa non-small-cell lung cancer.","volume":"86","author":"Roth","year":"1994","journal-title":"J Natl Cancer Inst"},{"key":"10.1378\/chest.113.6.1526_bib52","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1056\/NEJM199401203300301","article-title":"A randomized trial comparing preoperative chemotherapy plus surgery with surgery alone in patients with non-small-cell lung cancer.","volume":"330","author":"Rosell","year":"1994","journal-title":"N Engl J Med"}],"container-title":["Chest"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0012369216314945?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0012369216314945?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T07:36:24Z","timestamp":1757921784000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0012369216314945"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,6]]},"references-count":52,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1998,6]]}},"alternative-id":["S0012369216314945"],"URL":"https:\/\/doi.org\/10.1378\/chest.113.6.1526","relation":{},"ISSN":["0012-3692"],"issn-type":[{"value":"0012-3692","type":"print"}],"subject":[],"published":{"date-parts":[[1998,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Detection of Occult Micrometastases in Non-Small Cell Lung Carcinoma by Reverse Transcriptase-Polymerase Chain Reaction","name":"articletitle","label":"Article Title"},{"value":"Chest","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1378\/chest.113.6.1526","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 1998 The American College of Chest Physicians. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}